Bat-infesting chiggers (Acariformes: Trombiculidae) of the Balearic Islands and new data on the genus Trisetica Traub et Evans, 1950 Author Stekolnikov, Alexandr A. Zoological Institute of the Russian Academy of Sciences, Saint Petersburg, Russia; Author Quetglas, Juan Murciélagos y más, Espartinas, Spain text Folia Parasitologica 2019 017 2019-10-18 66 1 10 journal article 57474 10.14411/fp.2019.017 56731c8c-620c-431e-819a-c9432ea3a7c3 1803-6465 8178885 Trisetica knighti ( Radford, 1954 ) comb. n. Figs 2–8 Trombicula knighti – Radford (1954) : 298 , fig. 49; Kolebinova (1966) : 74 , figs 1–5. Trombicula ( Anomalaspis ) knighti – Vercammen-Grandjean and Nadchatram (1963) : 385. Sasatrombicula ( Rudnicula ) knighti – Vercammen-Grandjean (1965) : 68. Rudnicula knighti – Brown et al. (1988) : 17 . Sasatrombicula ( Rudnicula ) balcanica – Kolebinova (1966) : 74, figs 6–11, syn. n.; Kolebinova (1992) : 69, fig. 36. Rudnicula balcanica – Brown et al. (1988) : 17 . H o l o t y p e d e p o s i t i o n: National Museum of Natural History, Washington , DC, USA ( Radford 1954 , Goff 1989 ). D i a g n o s i s: SIF = 4B-N(b)-3(4)-3111.0000; fsp = 7.7.7; fCx = 1.1.1; fSt = 2.2; fPp = B/B/NNB; fSc: AM ≥ PL> AL; Ip = 817–952; fD = 2H-10-14(15)-9(11)-8(7)+(5–11); DS = 47–54; VS = 48–62; NDV = 102–109. Standard measurements are given in Table 1 . The re-description is based on one paratype from ZIN, one paratype from NHM (1952.7.23.9), and five additional specimens ( ZIN 11168–11172 ) . Re - de s c ri p ti on (la rv a) Fig. 3. Trisetica knighti ( Radford, 1954 ) , larval specimen ZIN 11170: scutum and dorsal idiosomal setae (sensilla missing). Abbreviations : AL – anterolateral scutal seta; AM – anteromedian scutal seta; H – humeral seta; PL – posterolateral scutal seta; SB – sensillary base. Fig. 2. Trisetica knighti ( Radford, 1954 ) , larva. A – arrangement of dorsal idiosomal setae in a paratype (ZIN); B – arrangement of ventral idiosomal setae in a paratype (ZIN; excretory pore is invisible); C – dorsal idiosomal seta in specimen ZIN 11168; D – ventral preanal idiosomal seta in specimen ZIN 11168. Scale bar 100 μm (A, B), 20 μm (C, D). Idiosoma Figs 2–4 Eyes 2 + 2. One pair of humeral setae and 45–52 barbed dorsal idiosomal setae; always 10 setae in 1 st row, 14 ( 15 in one additional specimen) setae in 2 nd row, 11 (in two additional specimens) or 9 (in one paratype and two additional specimens) setae in 3 rd row, 8 (in two additional specimens) or 7 (in one paratype and two additional specimens) setae in 4 th row, next 5–11 setae with ambiguous distribution by rows; four sternal setae; 48–62 ventral setae; total number of idiosomal setae ( NDV ), excluding coxal and sternal, 102–109 . Fig. 4. Trisetica knighti , larval specimen ZIN 11170: sternal area of idiosoma. Abbreviations : cxI – coxa I; cxII – coxa 2; cxIII – coxa III; ic – infracapitulum (gnathobase); sta – anterior sternal seta; stp – posterior sternal seta; V – ventral preanal setae. Gnathosoma Fig. 5 Cheliceral blade with tricuspid cap; gnathobase sparsely covered with rather large puncta and bears one pair of branched setae; galeal setae nude or having small cilium (in specimen ZIN 11169); palpal claw with 3 visible prongs (4 prongs in specimen ZIN 11168); palpal femoral and genual setae with long branches, dorsal and lateral palpal tibial setae nude, ventral palpal tibial seta branched; palpal tarsus with 4 branched setae and basal tarsala. A Fig 5. Trisetica knighti ( Radford, 1954 ) , larval specimen ZIN 11168. A – dorsal aspect of gnathosoma (cheliceral blade missing, only two prongs of palpal claw are visible); B – ventral aspect of gnathosoma (cheliceral blade missing, only two prongs of palpal claw are visible); C – palpal claw. Abbreviations : Ga – galeal seta; gns – gnathocoxal seta; ic – infracapitulum (gnathobase); PC – palpal claw; PF – palpal femur; PG – palpal genu; PTa – palpal tarsus; PTi – palpal tibia; S – palpal tarsala. 0 Table 1. Trisetica knighti ( Radford, 1954 ) : measurements (in μm) and counts.

Paratype (ZIN)	Paratype (NHM)	Specimens from Balearic Islands (n = 4) Range Mean		Sasatrombicula (Rudnicula) balcanica *
AW	51	47	51–58	55	47–65
SB	23	21	24–25	24	21–25
ASB	32	33	32–35	34	30–31
PSB	20	-	18–23	20	17
SD	52	-	52–57	54	47–48
AM	48	38	50–51	51	29(39**)
AL	35	34	31–40	35	35–44
PL	50	52	46–51	49	46–47
H	41	45	40–45	43	40–55
D min	-	30	26–31	30	34–35
D max	38	39	38–40	39	36–46
V min	-	-	23–27	24	26–28
V max	-	-	32–35	34	27–32
pa	290	299	319–335	328	286–295
pm	243	256	275–284	280	242–254
pp	284	296	324–338	332	269–274
Ip	817	851	918–952	940	809–811
DS	47	-	52–54	53	50
VS	62	-	48–55	53	50
NDV	109	-	102–109	106	82–100
TaIIIL	81	83	90–95	93	78**
TaIIIW	18	20	15–16	16	-

Notes: * – according to Kolebinova (1992) ; ** – our measurements based on the figures in original descriptions ( Kolebinova 1966 ). Abbreviations :AW – distance between anterolateral scutal setae; SB – distance between sensillary bases; ASB – distance from the level of sensillary bases to extreme anterior margin of scutum; PSB – distance from the level of sensillary bases to extreme posterior margin of scutum; SD – length of scutum (ASB + PSB); AM – length of anteromedian scutal seta;AL – length of anterolateral scutal setae; PL – length of posterolateral scutal setae; H – length of humeral setae; D min – length of the shortest dorsal idiosomal seta; D max – length of the longest dorsal idiosomal seta; V min – length of the shortest ventral idiosomal seta; V max – length of the longest ventral idiosomal seta; pa – length of leg I (including coxa); pm – length of leg II (including coxa); pp – length of leg III (including coxa); Ip – sum of leg lengths (pa + pm + pp); DS – number of dorsal idiosomal setae (including humeral); VS – number of ventral idiosomal setae (excluding coxal and sternal); NDV – number of idiosomal setae (DS + VS); TaIIIL – length of leg tarsus III; TaIIIW – width of leg tarsus III. Fig. 6. Trisetica knighti ( Radford, 1954 ) , larval specimen ZIN 11170: scutum (sensilla missing). Abbreviations : AL – anterolat- eral scutal seta; AM – anteromedian scutal seta; PL – posterolat- eral scutal seta; SB – sensillary base. Scutum Figs 3 , 6 , 7 Nearly hexagonal, with broadly rounded posterior margin and sparse large puncta; AM at level of ALs; PLs off scutum (peniscutum); anterior scutal angles with ALs tend to reduce; AM ≥ PL> AL; all scutal setae with pointed apices and covered with long barbs; sensilla densely covered with scale-like barbs in proximal half, slightly expanded and covered with large spikes in distal half. Legs Fig. 8 Fig. 7. Trisetica knighti ( Radford, 1954 ) , larval specimen ZIN 11171: scutum (one sensillum intact). Scale bar 25 μm. Abbreviations : AL – anterolateral scutal seta; AM – anteromedian scutal seta; S – sensillum; SB – sensillary base. All legs 7-segmented, with 1 pair of claws and claw-like empodium. Leg I: coxa with 1 non-specialised branched seta (1B); trochanter 1B; basifemur 1B; telofemur 5B; genu 4B, 3 genualae, microgenuala; tibia 8B, 2 tibialae, microtibiala; tarsus 22B, tarsala 20 μm long, microtarsala distal to tarsala, subterminala, parasubterminala, pretarsala. Leg II: coxa 1B; trochanter 1B; basifemur 2B; telofemur 4B; genu 3B, genuala; tibia 6B, 2 tibialae; tarsus 16B, tarsala 24 μm long, microtarsala proximal to tarsala, pretarsala. Leg III: coxa 1B; trochanter 1B; basifemur 2B; telofemur 3B; genu 3B, very long genuala; tibia 6B, very long tibiala; tarsus 16B.