Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China Author Li, Wen-Li School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China Author Liang, Rui-Ru https://orcid.org/0000-0001-7727-0998 School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China Author Dissanayake, Asha J. https://orcid.org/0000-0002-8061-8884 School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China Author Liu, Jian-Kui https://orcid.org/0000-0002-9232-228X School of Life Science and Technology, Center for Informational Biology, Electronic Science and Technology University, Chengdu 611731, China ljiankui@gmail.com text MycoKeys 2023 2023-05-23 97 71 116 http://dx.doi.org/10.3897/mycokeys.97.103118 journal article http://dx.doi.org/10.3897/mycokeys.97.103118 1314-4049-97-71 6292387F797752B1AE9167726B3A8D11 Diplodia mutila (Fr.) Mont., Ann. Sci. nat., ser . 2, 1: 302. 1834. Fig. 9 Sphaeria mutila Fr., Syst. Mycol. (Lundae) 2: 424. 1823. Basionym. Physalospora mutila ≡ Physalospora mutila (Fr.) N.E. Stevens, Mycologia 28: 333. 1936. Botryosphaeria stevensii = Botryosphaeria stevensii Shoemaker, Canad. J. Bot. 42: 1299. 1964. Description. Saprobic on decaying branches of Camellia oleifera . Sexual morph : Not observed. Asexual morph : Coelomycetous, Conidiomata 330-394 x 215-230 μm ( x̄ = 362 x 223 μm , n = 10), immersed, erumpent, gregarious, dark brown to black, subglobose, unilocular. Ostiole 48.5-67 μm diam., central. Peridium 29-38 μm wide, thick-walled, outer and inner layers composed of 1-2 layers dark brown textura angularis , intermediate layers composed of 3-5 layers of hyaline cells of textura angularis . Conidiophores reduced to conidiogenous cells. Conidiogenous cells 8.5-12 x 3-5 μm ( x̄ = 10 x 4 μm , n = 20), cylindrical, thin-walled, hyaline, holoblastic, indeterminate, proliferating at the same level to produce periclinal thickenings, or proliferating percurrently giving rise to 2-3 indistinct annellations. Conidia 19-21 x 9.5-11 μm ( x̄ = 20 x 10.5 μm , n = 30), L/W ratio = 2, oblong, with broadly rounded apex and truncate base, thick-walled, wall externally smooth, roughened on the inner surface, hyaline, aseptate, becoming dark brown when aged. Figure 9. Diplodia mutila (HUEST 22.0069, new host record) a, b appearance of conidiomata on natural substrate c vertical section of conidioma d ostiole e section of peridium f-i conidiogenous cells and developing conidia j hyaline immature conidium k-n mature brown conidia. Scale bars: 40 μm ( c ); 20 μm ( d, e ); 10 μm ( f-n ). Culture characteristics. Colonies on PDA initially olivaceous buff in the center of the colony and white at the edge, becoming olivaceous within 7 d on the surface, with smooth edge. Materials examined. China , Sichuan Province , Jiangyou City , Shuanghe County , 31°54'10"N , 104°55'57"E , elevation 657 m , on dead branches of Camellia oleifera , 11th July 2021 , W.L Li , 286 (HUEST 22.0069), living culture UESTCC 22.0068 ; ibid ., 289 (HUEST 22.0068), living culture UESTCC 22.0067 ; ibid ., Guangyuan city, Qingchuan County , 32°40'38"N , 105°28'57"E , elevation 634 m , on dead branches of Olea europaea , 20th April 2021 , W.L Li , 188 (HUEST 22.0065), living culture UESTCC 22.0064 ; ibid ., 257 (HUEST 22.0070), living culture UESTCC 22.0069 ; ibid ., on dead branches of Vernicia fordii , 20th April 2021 , W.L Li , 238 (HUEST 22.0066), living culture UESTCC 22.0065 ; ibid ., Chengdu City , Pidu District , 30°49'27"N , 103°47'42"E , elevation 442 m , on dead branches of Pistacia chinensis , 5th March 2021 , W.L Li , A61 (HUEST 22.0064), living culture UESTCC 22.0063. Additional sequences: LSU: OQ164832 (UESTCC 22.0063), OQ164830 (UESTCC 22.0064), OQ164831 (UESTCC 22.0065) . Notes. The phylogenetic tree show that six strains isolated from Camellia oleifera , Olea europaea and Vernicia fordii nested with Diplodia mutila (CBS 112553) with a moderate bootstrap support (ML/BI 86%/1). Diplodia mutila , the type of the genus, is a well-known and most commonly reported species. It has been recorded mainly from woody substrates, and it is known from more than 50 hosts ( Batista et al. 2021 ). Morphologically, one of the isolates obtained in this study UESTCC 22.0068 shares similar conidia shape and size with Di. mutila , but hardly observed the mature conidia with septa. We identify these taxa as Di. mutila based on morphology and phylogeny evidences. This is the first report of Di. mutila , isolated from Camellia oleifera , Olea europaea and Vernicia fordii .