Morphological redescription and DNA barcoding of Kaluginia lebetiformis Makarchenko, 1987 (Diptera: Chironomidae, Diamesinae) from South Korea Author Makarchenko, E. A. Author Semenchenko, A. A. Author Kang, H. Author Bae, Y. J. text Far Eastern Entomologist 2018 2018-09-10 367 26 32 http://dx.doi.org/10.25221/fee.367.4 journal article 10.25221/fee.367.4 2713-2196 7164552 4180A9D7-27F0-4A25-B6D2-D85705EA743E Kaluginia lebetiformis Makarchenko, 1987 Figs 1–10 Kaluginia lebetiformis Makarchenko, 1987: 786, 2006: 266 ; Oliver, 1989: 134; Ashe & Connor, 2009: 291. MATERIAL EXAMINED. South Korea : 2♂, Gyeongi-do, Gapyeong-gun, Gapyeong- cheon, Bukhan River, Han River basin, 15. IV 2016 (Light trap), 37°58’ N, 36.4” 127°26’ 35.5” E, leg. Y. Bae; 1♂, the same data except 22.V 2014 (Light trap), leg. H. Kang. Russia : South Sakhalin, 1♂ (holotype), Belaya River, Sokol Village, Dolinsk District, 29.VI 1985, leg. S. Bestalannaya, E. Makarchenko. REDESCRIPTION. Male imago (n=3). Total length 2.3–2.6 mm. Total length/wing length 1.03–1.10. Total coloration brown to dark-brown; antepronotum light yellow; methonotum yellowish, with brown stripes; legs spotted: basal 2/3 of femur yellowish, distal 1/3 brown; tibia in basal and apical parts brown and yellowish in middle part; basal 2/3 of ta 1 yellowish and distal 1/3 brown; ta 2 –ta 5 brown (Fig. 1). Head . Temporal setae (from one side) including 3–4 frontals, 7 orbitals and 7–10 verticals. Clypeus with 17–26 setae. Palpomere length (μm): 28–48, 60–64, 104–120, 132–140, 212– 220. Head width/palpal length 0.92–1.0. Antenna with 7 flagellomeres and reduced plume of setae 48–68 μm long (Fig. 2). Flagellomeres length (μm): 52–56, 24, 28, 24–28, 28–32, 28– 32, 68–84; terminal flagellomere with 2 subapical setae 60–76 μm long; scape with 3 setae 28–36 μm long. AR 0.34–0.46. Thorax . Antepronotum with 18–20 lateral setae. Acrostichals 25 (48–56 μm), dorsocentrals 21–27 (72–100 μm), prealars 16, supraalars 1, scutellars ac 50. Wing . Length 2.08–2.52 mm, width 0.68–0.76 mm. Anal lobe well developed; squama with 19–31 setae (64–80 μm) in 1–2 rows. R and R 1 with 55–58 setae (32–36 μm), R 4+5 with 24–29 setae (28–40 μm) in subapical part. Wing of one male with 3 short setae on M 3+4 . Legs . BR 1 2.0; BR 2 1.8; BR 3 1.9. Spur of front tibia 44 μm long. Spurs of middle tibia 28–52 and 44–53 μm long. Spurs of hind tibia 68–72 μm and 44–52 μm long. Hind tibial comb with 12–14 spine-like setae. Middle legs with 4 pseudospurs 36–40 μm long on ta 1 ; hind legs with 7 pseudospurs on ta 1 . Claw with 4 denticles apically. Length (µm) and pro- portions of legs segments are as follow:

P	fe	ti	ta 1	ta 2	ta 3	ta 4	ta 5	LR	BV	SV
P 1	918– 1115	1132– 1328	722– 886	394– 459	197– 213	66– 74	98– 115	0.64– 0.67	3.67– 3.87	2.76– 2.84
P 2	984– 1197	1082– 1246	574– 738	312– 394	189– 213	115– 74	115	0.53– 0.59	3.61– 4.0	3.31– 3.60
P 3	1000– 1214	1230– 1443	705– 886	394– 508	213– 246	66– 74	98– 131	0.57– 0.61	3.69– 3.81	3.0– 3.16

Hypopygium ( Figs 3–10 ). Tergite IX with “anal point” which like small rounded protuberance, and with 29–42 setae, 12–20 μm long; laterosternite IX with 15–20 setae (from one side), 44–48 μm long. Transverse sternapodeme high, almost trapezoidal, 128–132 μm long of specimens from South Korea , 92 μm long of specimen from South Sakhalin, and 148–152 μm wide in basal part, 88–100 μm wide in subapical part, with rounded apex ( Figs 4–5 ). Gonocoxite 308–310μm long. Basal lobe of gonocoxite is various shapes and depends on its position; in the inner half with microtrichia, along the outer margin with short setae ( Figs 3 , Figs 1–3. Adult male of Kaluginia lebetiformis Makarchenko from South Korea. 1 – total view of male, from one side; 2 – antenna; 3 – total view of hypopygium, from above. Figs 4–10. Hypopygium of Kaluginia lebetiformis Makarchenko from South Sakhalin (4) and South Korea (5–10). 4–5 – transverse sternapodeme and aedeagal lobe; 6–7 – basal lobe of gonocoxite; 8–9 – gonostylus; 10 – total view of hypopygium, from above. Scale bars = 50 µm. 6–7, 10); inferior volsella as in Figs 3 , 10 , covered by setae 40–60 μm long. Gonostylus 156 μm long, scoop-shaped; inner lobe along the margin with 5–9 megasetae 12–16 μm long and one subterminal tooth; outer lobe widely triangular, inner margin of which with strong setae 20–24 μm long, outer half with thinner and longer setae 56–72 μm long ( Figs 3 , 8–10 ). HR 1.97. REMARKS. Additional material from South Korea allowed a more detailed study of the internal structure of the male hypopygium, namely transverse sternapodeme, which in complex with aedeagal lobe, basal lobe of gonocoxite and inferior volsella is typical for Boreoheptagyiini . Males from both populations are close related by all features but specimens from South Korea have more a long transverse sternapodeme (128–132 μm) than specimen from South Sakhalin (92 μm). DISTRIBUTION. East Palaearctic continental-insularis species known from Sakhalin Island and Primorye Territory in Russia and from South Korea .