Taxonomy and phylogeny of Smaragdiniseta musae sp. nov. and Albifimbria verrucaria (Hypocreales, Stachybotryaceae) on Musa from Thailand Author Samarakoon, Binu C. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand Author Wanasinghe, Dhanushka N. https://orcid.org/0000-0003-1759-3933 CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, China & Centre for Mountain Futures (CMF), Kunming Institute of Botany, Chinese Academy of Sciences, Honghe 654400, Yunnan, China Author Bhat, Jayarama Department of Botany, Goa University, Goa, India Author Chomnunti, Putarak School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand putarak.cho@mfu.ac.th text Biodiversity Data Journal 2022 2022-07-27 10 89360 89360 http://dx.doi.org/10.3897/BDJ.10.e89360 journal article http://dx.doi.org/10.3897/BDJ.10.e89360 1314-2828-10-e89360 0F89E4CCAE9F5493BFC88E4844D35E1C Albifimbria verrucaria (Alb. & Schwein.) L. Lombard & Crous Materials Type status: Other material . Occurrence : occurrenceRemarks: Found on a dead leaf of Musa sp.; recordNumber: BNS292; recordedBy: Binu C. Samarakoon ; disposition: Living culture: MFLUCC 22-0017; associatedSequences: GenBank : MFLUCC 22-0017: ON563487 (ITS), ON586153 (tub2); Taxon : scientificName: Albifimbria verrucaria ( Alb. & Schwein. ) L. Lombard & Crous ; kingdom: Fungi ; phylum: Ascomycota ; class: Sordariomycetes ; order: Hypocreales ; family: Stachybotryaceae ; genus: Albifimbria ; specificEpithet: verrucaria; taxonRank: species; scientificNameAuthorship: ( Alb. & Schwein. ) L. Lombard & Crous ; Location : continent: Asia ; country: Thailand ; stateProvince: Chiang Rai ; municipality: Mae Sai ; Identification : identifiedBy: Binu C. Samarakoon ; Event : year: 2019; month: June ; day: 25; habitat: Terrestrial ; Record Level : institutionID: MFLU; collectionID: MFLU 22-0048 Description Saprobic on dead leaves of Musa sp. Sexual morph : Undetermined. Asexual morph : Sporodochia cupulate or discoid, scattered or gregarious, having irregular or rounded outline composed of white marginal hypha, with conidial mass flattened or convex, pale olivaceous-green at an immature stage, black and shiny at maturity, 10-18 x 0.8-3 μm (x̄ = 12.3 x 2.4 μm , n = 20). Stroma rarely well-developed, usually with a thin layer of isodiametric or elongated hyaline cells 15-25 (x̄ = 16.8 μm , n = 10). Setae: not observed. Marginal hyphae hyaline, usually verrucose, septate, curling and coiling, some branched, rounded or blunt at apex, 1.5-4 (x̄ = 3.3, n = 20) in diam. Conidiophores arising from a thin stromatic layer, hyaline, smooth, 30-48 x 1-2 μm (x̄ = 42.2 x 1.7 μm , n = 30) septate, branching repeatedly, forming 2-4 branches at each level, with ultimate branches becoming phialides, which give rise to numerous conidia, conidiophores sometimes also arising from the hyphae. Phialides hyaline, rough-walled 30-48 x 1-2 μm (x̄ = 42.2 x 1.7 μm , n = 30), 3-7 in a whorl, closely packed in a dense parallel layer, cylindrical, hyaline, collate at the base, rounded or acute at apex, sometimes slightly tapering towards apex, 8-16 x 1-3.5 μm (x̄ = 12.2 x 1.8 μm , n = 30). Conidia broadly fusiform, always pointed at one end, mostly truncate or rounded at the other end, hyaline, sometimes sub hyaline, smooth, 5-9.5 x 2-3.5 μm (x̄ = 7.6 x 3.0 μm , n = 30). Culture characteristics. Conidia germinated on PDA after 12 hours. Colonies growing on PDA reaching 40 mm diam. after 2 weeks in the light conditions at 25°C, mycelium is mostly immersed, not slimy, cottony, white, dense in the middle and comparatively sparse at the periphery, fast-growing. Sporodochia formed after 12 days at the centre as a black uneven ring. Notes Based on BLASTn search results of ITS, tub2 and rpb2 sequence data, our stain (MFLUCC 22-0017) showed a high similarity (ITS = 100% tub2 = 100% and rpb2 = 99%), excluding gaps to Albifimbria verrucaria (CBS 188.46). In the multigene phylogeny, MFLUCC 22-0017 grouped with A. verrucaria strains with strong statistical supports (97% ML, 1.00 BYPP) (Fig. 3 ). Morphologically, our collection (Fig. 2 ) is similar to the descriptions of Tulloch (1972) and Lombard et al. (2016) . Albifimbria verrucaria has previously been reported from Musa sp. as a saprobe in Venezuela ( Dennis 1970 ). This is the first report of Albifimbria from Thailand. MFLUCC 22-0017 is the first saprobic A. verrucaria strain found in Thailand. In addition, this is the second report of Albifimbria on Musaceae .