Strong differentiation between amphibian communities on two adjacent mountains in the Upper Rio Pastaza watershed of Ecuador, with descriptions of two new species of terrestrial frogs Author Reyes-Puig 1,2, Juan Pablo Unidad de Investigacion, Instituto Nacional de Bio-diversidad (INABIO), Quito, Ecuador Author Reyes-Puig 1,3,4, Carolina Unidad de Investigacion, Instituto Nacional de Bio-diversidad (INABIO), Quito, Ecuador Author Franco-Mena 5,6, Daniela Unidad de Investigacion, Instituto Nacional de Bio-diversidad (INABIO), Quito, Ecuador Author Jost 2, Lou Unidad de Investigacion, Instituto Nacional de Bio-diversidad (INABIO), Quito, Ecuador Author Yanez-Munoz 1,2, Mario H. Unidad de Investigacion, Instituto Nacional de Bio-diversidad (INABIO), Quito, Ecuador text ZooKeys 2022 2022-01-10 1081 35 87 http://dx.doi.org/10.3897/zookeys.1081.71488 journal article http://dx.doi.org/10.3897/zookeys.1081.71488 1313-2970-1081-35 40218EB45A244B0691B8402CBAFF9062 EB35434016A458FC997D6E708136C163 Pristimantis tinguichaca Brito M, Ojala-Barbour, Batallas R & Almendariz C, 2016 Figure 18 Remarks. We recorded a female specimen (DHMECN 14426) with body size of 28.83 mm collected on a palm leaf in the montane forests of the Machay Reserve at 2290 m elevation. This specimen corresponds to the first record of P. tinguichaca on the north side of the Rio Pastaza and extends its altitudinal distribution in the Province of Tungurahua from the previous record of 2470 m ( Brito et al. 2016 ; Franco-Mena et al. 2019 ). This species can be distinguished by the presence of small conical tubercles on the upper eyelids and heels and by its red-coloured iris. Figure 18. Pristimantis tinguichaca , Female (DHMECN 14426) A lateral view B dorsal view C frontal view D dorsal view. Photographs by Mario H. Yanez-Munoz .