Annotated checklist of freshwater molluscs from the largest freshwater lake in Southeast Asia Author Ng, Ting Hui Lee Kong Chian Natural History Museum, Faculty of Science, National University of Singapore, 2 Conservatory Drive, 117377, Singapore https://orcid.org/0000-0002-5123-0039 Author Jeratthitikul, Ekgachai Animal Systematics and Molecular Ecology Laboratory, Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand https://orcid.org/0000-0002-3477-9548 Author Sutcharit, Chirasak Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand Author Chhuoy, Samol Inland Fisheries Research and Development Institute (IFReDI), Fisheries Administration, No. 86, Norodom Blvd., PO Box 582, Phnom Penh, Cambodia & Wonders of the Mekong Project, c / o IFReDI, No. 86, Norodom Blvd., PO Box 582, Phnom Penh, Cambodia Author Pin, Kakada Wonders of the Mekong Project, c / o IFReDI, No. 86, Norodom Blvd., PO Box 582, Phnom Penh, Cambodia Author Pholyotha, Arthit Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand https://orcid.org/0000-0001-6677-1164 Author Siriwut, Warut Animal Systematics and Molecular Ecology Laboratory, Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand Author Srisonchai, Ruttapon Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand Author Hogan, Zeb S. Wonders of the Mekong Project, c / o IFReDI, No. 86, Norodom Blvd., PO Box 582, Phnom Penh, Cambodia & Department of Biology, University of Nevada, 1664 N. Virginia Street, Reno, NV 89557, USA Author Ngor, Peng Bun Inland Fisheries Research and Development Institute (IFReDI), Fisheries Administration, No. 86, Norodom Blvd., PO Box 582, Phnom Penh, Cambodia & Wonders of the Mekong Project, c / o IFReDI, No. 86, Norodom Blvd., PO Box 582, Phnom Penh, Cambodia https://orcid.org/0000-0002-3659-6577 pengbun.ngor@gmail.com text ZooKeys 2020 958 107 141 http://dx.doi.org/10.3897/zookeys.958.53865 journal article http://dx.doi.org/10.3897/zookeys.958.53865 1313-2970-958-107 AB196008154249D4B23E1892D2191C18 377C3EF18E8951FD9599616E45E03C94 Pomacea maculata Perry, 1810 Fig. 5D Pomacea maculata Perry, 1810: unnumbered plate and text. Type locality: Parana , Argentina (see discussion in Hayes et al. 2012 ). Material examined. CIFI.MOL.037, ZRC.MOL.015691, ZRC.MOL.015695, ZRC.MOL.015692, ZRC.MOL.015693, ZRC.MOL.015694. Distribution and habitat. Tonle Sap River and Lake, and paddy fields in Banteay Meanchey and Kampong Thom Provinces (locality no. 1, 2, 5, 7, 15, 22, 38, 40, and 44). Remarks. Unlike Pila species, Pomacea maculata is not native to Southeast Asia. Pomacea maculata (as Pomacea insularum d'Orbigny , 1835 in Hayes 2008), and another species, Pomacea canaliculata (Lamarck, 1822), have both been introduced to Asia from South America ( Hayes et al. 2012 ; Joshi et al. 2017 ). Molecular methods are the most accurate way to distinguish between them (Rama Rao et al. 2018), and DNA barcodes of two individuals from the Tonle Sap basin were a match to Pomacea maculata (GenBank Accession No. MT372328, MT372329). Because of the morphological similarity between the two species, some records of Pomacea canaliculata in Southeast Asia, including in Cambodia, may instead have been of Pomacea maculata (see Cowie and Hayes 2012 ). Pomacea species were first recorded in Cambodia only in the mid-1990s (compared to early 1980s in neighbouring Thailand), and even then, had only been collected from three localities ( Cowie 1995 ). From then onwards, Pomacea spp. may have spread because these snails were mistaken for native ampullariids and were translocated to paddy fields in attempts to breed them for food - unfortunately, Pomacea spp. became pests that destroyed the crops instead ( Khay et al. 2018 ). At present, Pomacea maculata appears to be widespread in the Tonle Sap basin.