New species, redescriptions and a new combination of Acanthocyrtus Handschin, 1925 and Amazhomidia Cipola & Bellini, 2016 (Collembola, Entomobryidae, Entomobryinae) Author Cipola, Nikolas Gioia Author Morais, José Wellington De Author Bellini, Bruno Cavalcante text Zootaxa 2018 2018-02-28 4387 3 401 435 journal article 30631 10.11646/zootaxa.4387.3.1 719ac0a1-94e1-4f6b-9301-2f88113caa87 1175-5326 1187567 41E75EE7-74FD-471F-B7E8-937C00110B6A Amazhomidia thaisae sp. nov. Cipola & Bellini Figs 9‒16 Type material. Holotype female on slide (067/ INPA ): Brazil , Amazonas , Manaus municipality, Reserva Ducke , grid L8-3500, 02°59’05”S , 59°55’42”W , Amazon forest , 67 m , 23.ix-04.xi.2014 , pitfall-trap of 48 hours, T Vicente coll . 6 paratypes on slides (067A‒F/INPA): 2 males , 3 females and 1 juvenile and 6 specimens in alcohol, same data as holotype . 1 paratype in alcohol (INPA): same data as holotype, except grid L5-4500, 02°57’24”S , 59°55’24”W , 113 m . 1 paratype in alcohol ( INPA ) : same data as holotype, except grid L5-2500, 02°57’35”S , 59°56’30”W , 102 m , 15.ix-20.v.2006 , JLP Souza coll. Diagnosis. Body dark yellow with dark purple bands on Ant II and Abd IV and femora distally ( Fig. 9 ); appendages unscaled; Ant IV without apical bulb; Ant III with one subapical scale-like chaeta; Ant I dorsally with 4 median mac; prelabral inner chaetae smooth and bifurcated equally; Gr. II‒III of head with 5‒6 and 3 mac respectively, M1 and S1 mac absent; basomedian labial field without M2 ; cephalic groove with 1+1 scale-like chaetae ( Figs 10 , 13B‒D ); Th II without m2 , m 2i and m4p mac; Th III‒Abd III with 12‒13, 4, 4‒5 and 3 mac respectively; Abd IV with 16‒26 median mac and 10 lateral mac ( Figs 14 , 15A‒D ); unguiculi I‒II truncate, III weakly truncate; manubrium ventrally with 28 apical chaetae and 5‒6 long distal mac on lateral side; manubrial plate with 7 chaetae and dens with 22‒33 dorsal spines ( Figs 12A‒C , 16G‒I ). FIGURE 9. Amazhomidia thaisae sp. nov. : hAbITUS Of hOLOTYPE fIXED IN ALcOhOL (LATERAL VIEW). ScALE bAR: 0.5 MM. Description. Total length (head + trunk) of specimens 2.04‒2.36 mm (n=4), holotype 2.04 mm . Body with dark yellow color and dark purple pigment on distal two thirds of the Ant IV, distal and proximal Ant III, distal Ant I‒II weakly, Abd IV and femora distal bands and dorso-proximal manubrium; eyepatches black ( Fig. 9 ). Scales heavily ciliated, short or elongated and apically pointed on dorsal and lateral head, thorax and abdomen dorsally. Antennae, legs, collophore and furcula unscaled. Head. Antennae slightly longer than trunk length, ratio antennae: trunk = 1: 0.84; antennal segments ratio as I: II: III: IV = 1: 1.69: 1.32: 2.47 ( Fig. 9 ). Ant IV annulated on distal three quarters, without apical bulb, with s-blunt sens of different sizes and ciliate chaetae ( Figs 3A‒B ). Ant III sense organ with 2 rods, 4 spiny guard sensilla, with s-blunt sens of different sizes and ciliate chaetae; subapical scale-like chaeta present (as in Figs 3C and 13A ). Ant I dorsally with 4 median mac, 5 distal mes and 3 smooth mic at base ( Fig. 13B ). Eyes 8+8, G and H smaller, A and B larger, with 5 interocular chaetae ( s absent). Head dorsal chaetotaxy ( Fig. 13C ) with 9 ‘An’, 4 ‘A’ ( A3 mac or mic), 3 ‘M’ ( M1 absent), 7‒8 ‘S’ ( S1 absent, S2e sometimes absent), 2 ‘Ps’, 3 ‘Pi’ ( Pi1 displaced forwards or backwards), 4‒5 ‘Pa’ ( Pa1 rarely present), 2 ‘Pm’, 4 ‘Pp’ ( p4 absent) and 2‒3 ‘Pe’ ( Pe3p sometimes absent) chaetae, plus 3 lateral mac of uncertain homology. Clypeal formula with 4 (l1‒2), 8 (frontal), 3 (pf0‒1) chaetae finely ciliate, l1‒2 and pf0‒1 larger, 3 frontal smaller and ciliate, others subequal. Four prelabral smooth chaetae, internal pair bifurcated equally ( Fig. 13D ); labral formula with 4 ( a1–2 ), 5 ( m0–2 ), 5 ( p0–2 ) smooth chaetae, a1 not thickest, p0–2 largest. Labral papillae absent. Labial palp with five main papillae (A‒E) plus one hypostomal papilla (H) with 0, 5, 0, 4, 4, 2 guard appendages, respectively, lateral process (l.p.) of papilla E finger-shaped and not reaching the papilla base; labium with five smooth proximal chaetae (p.c.) (as in Figs 25B‒C ). Maxillary palp with smooth apical appendage (a.a.) and basal chaeta (b.c.) subtly ciliated, thicker and 1.16 longer than the apical; sublobal plate with tree smooth appendages ( Fig. 18C ). Basomedian and basolateral labial fields with chaetae a1-5, m1 , r , e , l1‒2 smooth, r smaller present or not ( Fig. 10A ). Ventral head with numerous chaetae finely ciliate of different sizes, 8 anterior postlabial chaetae; cephalic groove with 1+1 scale-like chaetae and 10‒11 surrounding chaetae finely ciliate, 1 median chaeta larger and 1‒2 smaller, others subequal ( Figs 10A‒B ). FIGURE 10A–B. Amazhomidia thaisae sp. nov. : VENTRAL hEAD (LEfT SIDE); A , LAbIAL PALP, PROXIMAL chAETAE, bASOMEDIAN AND bASOLATERAL fIELDS AND PART Of ANTERIOR POSTLAbIAL chAETOTAXY; B , cOMPLETE chAETOTAXY, ARROWS INDIcATES ScALE-LIKE chAETAE ON cEPhALIc GROOVE ScALE bARS: 0.05 MM. FIGURE 11A–B. Amazhomidia thaisae sp. nov. : A , chAETOTAXY Of LEfT LEG I (POSTERIOR SIDE); B , cOLLOPhORE ANTERIOR AND LATERAL fLAP chAETOTAXY. ScALE bARS: A (0.1 MM), B (0.05 MM). Thorax chaetotaxy ( Figs 14A‒B ). Th II (anterior chaetal collar omitted) series ‘a’, ‘m’ and ‘p’ with 10‒11 (5 of uncertain homology), 4‒6 and 9‒14 mac respectively. Th III, series ‘a’, ‘m’ and ‘p’ with 4 ( a3 absent), 1 ( m6 absent) and 7‒8 mac respectively, p1 absent on one side in holotype. Ratio Th II: III = 1.73‒1.43: 1 (n=4), holotype 1.43: 1. Abdomen chaetotaxy ( Figs 14C‒E , 15A‒E ). Abd I with 3 central ( m2‒4 ) and 1 lateral ( m5 ) mac. Abd II with 2‒3 central mac ( m3‒3e , 3ep ) and 2 lateral mac ( m5‒6 ?), bothriotricha a5 and m2 with 11 and 8 accessory chaetae respectively. Abd III with 1 central mac ( m3 ) and 2 lateral mac ( pm6 and p6 ), bothriotricha a5 , m2 and m5 with 10, 9 and 12 accessory chaetae respectively. Abd IV with 7‒10 anteromedian mac, 9‒16 posteromedian mac ( holotype with 9-10, A4 absent), 10 lateral mac, about 12 mes (3 anterolateral and 9 posterior), about 10 posterior sens ( as? and ps type I, others type II) and approximately 16 posterior mes. Abd V series ‘m’ and ‘p’ with 4 and 3 mac respectively. Ratio Abd III: IV = 1: 6.40‒7.59 (n= 4), holotype 1: 7.59. FIGURE 12A–D. Amazhomidia thaisae sp. nov. : fURcULA; A , DISTAL MANUbRIUM AND PROXIMAL DENS (DORSAL SIDE); B , DISTAL MANUbRIUM (LATERAL SIDE), ARROWS INDIcATES INSERTIONS Of LONG DISTAL MAc fINELY cILIATE; C , PROXIMAL PART Of RIGhT DENS (DORSAL VIEW), ARROW INDIcATES INNER ROW Of SPINES; D , DISTAL DENS AND MUcRO. ScALE bARS: 0.05 MM. FIGURE 13A–D. Amazhomidia thaisae sp. nov. : DORSAL hEAD; A , ANT III APIcAL ORGAN (LATERAL SIDE); B , chAETOTAXY Of LEfT ANT I (DORSAL SIDE); C , cEPhALIc chAETOTAXY, ARROW INDIcATES VARIATION IN MAc PATTERN; D , chAETOTAXY Of PRELAbRUM AND cLYPEUS. FIGURE 14A–E. Amazhomidia thaisae sp. nov. : DORSAL chAETOTAXY; A , Th II, ARROW INDIcATES VARIATION IN MAc PATTERN (RIGhT SIDE); B , Th III; C , AbD I; D , AbD II; E , AbD III. FIGURE 15A–E. Amazhomidia thaisae sp. nov. : DORSAL chAETOTAXY; A , AbD IV; B‒D , POSTERIOR MAc PATTERN Of AbD IV IN PARATYPES: B , RIGhT AND LEfT SIDES, C‒D , LEfT SIDE; E , AbD V. FIGURE 16A–I. Amazhomidia thaisae sp. nov. : A , SUbcOXA I; B , SUbcOXA II; C , SUbcOXA III (OUTER SIDE); D , TROchANTERAL ORGAN (POSTERIOR SIDE); E , TROchANTER ANTERIOR chAETOTAXY, ARROW INDIcATES SPINE-LIKE chAETAE PRESENT OR AbSENT; F , cOLLOPhORE ANTERIOR AND LATERAL fLAP chAETOTAXY; G , MANUbRIUM VENTRO-DISTAL chAETOTAXY; H , MANUbRIUM DORSO-DISTAL chAETOTAXY, LATERAL ARROW INDIcATES chAETAE PRESENT OR AbSENT; I , DORSO-PROXIMAL DENS (LEfT SIDE). TABLE 1. Comparison of Amazhomidia species.

GENERA	A. ducke 3	A. guianiensis comb. nov. 1-2	Amazhomidia SP. 1	A . thaisae sp. nov.
ANT IV bULb	‒	+	‒	‒
ANT III ScALE-LIKE chAETA	+	?	+	+
ANT I MEDIO-DORSAL MAc	7	4‒5	4	4
ANT I ScALES	‒	+	+	‒
ANT II ScALES	‒	‒	+	‒
FEMUR ScALES	‒	‒	+	‒
MANUbRIUM VENTRAL ScALES	‒	‒	+	‒
A3	*	‒	‒	+/‒
M1	‒	‒	+	‒
HEAD DORSAL MAc	S1	‒	‒	+	‒
S2E	+	‒	+	+/‒
GR. II	4	4	5	5‒6
GR. III	3	1	3	3
PRELAbRAL chAETAE	INNER	B EqUALLY	B EqUALLY	B UNEqUALLY	B EqUALLY
OUTER	B OR NORMAL	NORMAL	NORMAL	NORMAL
LAbIAL	M1	+	+	+	+
M2	‒	‒	+	‒
chAETAE	R	S OR C	S	S	S OR ‒
ScALE-LIKE chAETAE ON VENTRAL hEAD	+	‒	‒	+
‘A’	28‒29	17	29	10‒11
Th II MAc	‘M’	8	7‒8	10	4‒6
‘P’	9‒16	11	16	9‒13
‘A’	3‒4	3	4	3
Th III MAc	‘M’	1‒2	2	3	2
‘P’	7‒10	8‒9	9	7‒8
AbD I MAc (MEDIAN/LATERAL)	3‒4/ 1	4/ 1	3‒4/ 2	3/ 1
AbD II MAc (MEDIAN/LATERAL)	2‒4/ 3	3/ 3	4/ 3	2‒3/ 2
AbD III MAc (MEDIAN/LATERAL)	1/ 3	1/ 5	1/ 4	1/ 2
AbD IV MAc (ANTERO/ POSTEROMEDIAN)	10/ 13‒17	10/ 7‒9	18‒19/ 11	7‒10/ 6‒16
AbD IV LATERAL MAc	10‒11	9	14	10
AbD IV POSTERIOR SENS	15	6	7	10
AbD IV POSTERIOR MES	20	28	33	16
TROchANTERAL ANTERIOR SPINE-LIKE chAETAE	10	4	6	6‒7
COLLOPhORE ANTERIOR chAETAE	28	16	32	23
COLLOPhORE LATERAL fLAP	12	10	21	14
MANUbRIUM VENTRAL chAETAE	38	33	68	28
MANUbRIUM LATERAL DIffERENTIATED MAc	5	5	7‒8	5‒6
MANUbRIAL PLATE chAETAE/PSP	11/ 2 PSP	9/ 2 PSP	16‒17/ 3 PSP	7/ 2 PSP
DENTAL SPINES	38	36‒44	45‒49	22‒33

Notes: (C) ciliated chaeta(e); (S) smooth chaeta(e); (B) bifurcate chaeta(e); (psp) pseudopore; (+) present; (–) absent. Reference: (1) Womersley 1930, (2) Zhang et al. 2009, (3) Cipola et al. 2016. * A3 chaeta presented in Cipola et al. 2016 is actually A2 . Legs. Subcoxa I (outer side) with 2 chaetae and 2 psp; subcoxa II with an anterior row of 9 chaetae plus about 12 anterior chaetae, posterior row of 3 chaetae and 4 psp; subcoxa III with one row of 7‒8 chaetae and 2 posterior psp ( Figs 16A‒C ). Trochanteral organ with about 25 spine-like chaetae, 2 arms posteriorly; anterior face with 6‒7 spine-like chaetae ( Figs 16D‒E ). Femora I‒III internally with 2 mac (proximal and median) and 1 distal mes finely ciliate and apically acuminate; tibiotarsi I‒III internally with 1 proximal mac and 1 median mes finely ciliate and apically slightly acuminate, others subequal ( Fig. 11A ). Pretarsus with one minute smooth chaeta on anterior and posterior sides. Ungues outer side with a pair of large laterobasal teeth; inner side with 3 teeth, 2 paired basal and 1 unpaired median slightly larger to basal teeth, apical tooth absent. Unguiculi I‒II truncate, unguiculus III weakly truncate, all with one small proximal external tooth, other smooth edges; ratio unguis: unguiculus III = 1: 0.58. Tibiotarsus III distally with one inner smooth chaeta 1.26 larger than unguiculus; outer side with tenent hair capitate, smooth and 0.85 smaller than unguis. Collophore. Anterior side with about 23 chaetae finely ciliate, distally 3 mac; posterior side with numerous chaetae finely ciliate, 1 unpaired chaeta medially larger, one pair distally smooth; lateral flap with about 14 smooth chaetae ( Figs 11B , 16F ). Furcula. Manubrium ventrally with 2 subapical and about 28 apical chaetae ciliate per side; manubrial plate with 7 chaetae finely ciliate of different sizes and 2 psp; lateral side with 5‒6 long distal mac finely ciliate ( Figs 12A‒B , 16G‒H ). Basal two thirds of dens with 22‒33 ( holotype with 32‒33) dorsal spines striated on irregular inner row; dorsal side with three irregular rows of chaetae finely ciliate, proximal chaetae larger ( Figs 12A and C , 16I ). Mucro bidentate with apical tooth subequal to proximal tooth, basal spine surpassing the apex of the proximal tooth ( Fig. 12D ). Etymology. The new species is named after Msc. Thais Vicente (INPA), friend of the first author and collectors of the specimens. Remarks. Amazhomidia thaisae sp. nov. resembles Amazhomidia ducke Cipola & Bellini, 2016 by appendages unscaled, prelabral inner chaetae bifurcated equally, head cephalic groove with 1+1 scale-like chaetae, empodial complex shape and mucro bidentate with basal spine. However, A. thaisae sp. nov. differs from this species by dark yellow body color with dark distal bands on Abd IV and femora, while A. ducke has a completely blue body with some depigmented regions. Amazhomidia thaisae sp. nov. differs also by Ant I dorsally with 4 median mac ( 7 in A. ducke ), manubrium ventrally with 28 apical chaetae ( 38 in A. ducke ), manubrial plate with 7 chaetae ( 11 in A. ducke ), and dens with 22‒33 dorsal spines ( 38 in A. ducke ). The dorsal chaetotaxy of A. thaisae sp. nov. differs by 5‒6 mac on Gr. II of the head ( 4 in A. ducke ), Th II with 10‒11 anterior mac ( 28‒29 in A. ducke ), and Abd III with 2 lateral mac ( 3 in A. ducke ). Other comparisons among species are presented in Table 1 .