A new species of pencil smelt Nansenia boreacrassicauda (Microstomatidae, Argentiniformes) from the North Atlantic Ocean Author Poulsen, Jan Yde text Zootaxa 2015 4020 3 517 532 journal article 10.11646/zootaxa.4020.3.6 9f7683c5-94a3-463a-aa3c-7bf0e9c26b23 1175-5326 238261 13C4AF61-8BC8-44D5-B448-194AEEBCCFCC Nansenia boreacrassicauda Poulsen , new species Figs. 1 A–D; Table 1 Type materials. Holotype . ZMUC P192172, 232.0 mm SL ( 65°32'N , 30°57'W ). Paratypes . ZMH 122532 ( ISH 174-1986), 197.2 mm SL ( 57°46'N , 18°5'W ); ZMH 120802 ( ISH 968-1982), 265.2 mm SL ( 49°48'N , 25°54'W ); ZMH 122530 ( ISH 264-1986), 226.0 mm SL ( 57°34'N , 18°12'W ); ZMH 122553 ( ISH 201-1986), 230.8 mm SL ( 57°43'N , 17°40'W ); ZMH 120833 ( ISH 967-1982), 268.0 mm SL ( 43°42'N , 28°27'W ). Comparative materials. Nansenia ardesiaca . AMS I.22817-025, 129.3 mm SL (Indian Ocean, off the Australian Northwest Shelf); BSKU 28403, 134.5 mm SL (Okinawa Through, Okinawa, Japan ). Nansenia cf. ardesiaca (adipose fin not pigmented). AMS I.22817-025, 124.2 mm SL (Indian Ocean, off the Australian Northwest Shelf); BSKU 13127, 155.0 mm SL (Northwestern Pacific Ocean, off Kochi , Japan ); MCZ 159274, 82.6 mm SL ( 31°26'N , 16°28'W ). Nansenia atlantica . ZMH 103928 ( ISH 335-1966), 90.8 mm SL ( 10°46'N , 23°54'W ); ZMH 105441 ( ISH 457- 1968), 110.8 mm SL ( 16°14'N , 22°24'E ); ZMH 105453 ( ISH 686-1968), 152.0 mm SL ( 08°21'N , 24°10'W ); ZMH 105485 ( ISH 1311-1968 ), 169.0 mm SL ( 16°08'N , 22°22'W ); AMS I.20066-072, 81.1 mm SL (off Brush Island, New South Wales , Australia ). Nansenia iberica ( paratype ). UAB 1985.6.14, 237.0 mm SL (Northwestern Mediterranean Ocean, off Blanes, Spain ). Nansenia oblita . MCZ 66132(2), 43.6 and 77.3 mm SL ( 37°01'N , 00°05'W ); MCZ 66134, 87.8 mm SL ( 39°57'N , 06°43'E ). Nansenia cf. oblita (adipose base pigmented). MCZ 66133, 59.3 mm SL ( 14°30'N , 21°47'W ). Nansenia cf. pelagica (vertebrae 46). ZMH 120958 ( ISH 465-1982), 106.5 mm SL ( 45°24'N , 27°41'W ). Nansenia tenera . MCZ 66016, 96.4 mm SL ( 49°53'N , 39°18'W ). The complete mitochondrial DNA sequences (mitogenomes), except a part of the control region, were determined for the holotype of N. boreacrassicauda sp. n. as well as a southeast Greenland specimen ( 64°57'N , 34°27'W ) of Argentina silus ( ZMUC P192172 and P192171, respectively). They were determined using conventional lab-methods employing DNA extraction (Qiagen Puregene kit), long-amplification (LA) PCR of the entire mitochondrial genome ( Cheng et al. 1994 ), nested short PCR using the LA PCR product as template, direct cycle-sequencing with dye-labeled terminators and Sanger Sequencing on automated DNA sequencers (Applied Biosystems). Lab-work and sequencing were carried out at the Ocean Research Institute ( ORI ), University of Tokyo, and at the Biodiversity Laboratory and Sequencing Facility at the University of Bergen (UIB). Universal fish primers covering a range of teleost fishes were employed (e.g. Miya & Nishida 1999 ; Byrkjedal et al. 2011 ).