The non-Siphonophoran Hydrozoa (Cnidaria) of Salento, Italy with notes on their life-cycles: an illustrated guide Author Gravili, Cinzia Author Vito, Doris De Author Camillo, Cristina Gioia Di Author Martell, Luis Author Piraino, Stefano Author Boero, Ferdinando text Zootaxa 2015 3908 1 1 187 journal article 42365 10.11646/zootaxa.3908.1.1 6f1d1977-6b97-4789-828c-76ed250cf1ae 1175-5326 242729 D6AD2B49-170B-4D9C-84AA-DBE0FEEAD8BE Amphinema bouilloni Schuchert, 2007 Fig. 28 A–C See Schuchert (2007) for a complete synonymy. Material examined. HCUS-S 0 33 (Hydrozoa Collection, University of Salento—fauna of the Salento Peninsula)—polyp stage; IG 27838 (see Bouillon et al. 1997)—medusa stage. Description (based on our own observations; Boero et al. 1997b as Codonorchis octaedrus ; Schuchert 2007): Hydroid. Hydrorhiza as creeping stolons; colonies stolonal; hydranths fusiform, sessile, small, 0.25 mm, naked; hypostome short, conical; with 4–6 filiform tentacles in a single whorl; medusa buds each borne on a pedicel of variable length but generally longer than hydranth, arising from hydrorhiza. Colours: hydranth yellow, hypostome white; medusa buds orange. Habitat type and substrate. Marine cave, on organogenic concretions; depth 25 m ( Boero et al. 1997b ). Seasonality. September ( Boero et al. 1997b ). Reproductive period. September ( Boero et al. 1997b ). FIGURE 28. Amphinema bouilloni : A , hydranth and medusa bud; B , newly released medusa; C , mature seventy-five-day old medusa (modified after Boero et al. 1997b). Scale bars: A, 0.2 mm; B, 1.0 mm; C, 2.5 mm. Medusa. Adult. Umbrella 2.5 mm wide, 4 mm high, with a high conical or globular apical projection; manubrium urn-shaped, with wide base, with an apical chamber, without gastric peduncle; mouth cruciform, with 4 simple recurved lips; with mesenteries; 4 broad ribbon-like radial canals with jagged edges; gonads on interradial walls of manubrium only, separated perradially, large, horse-shoe shaped, with some interradial folds; up to 16 marginal bulbs, 2 of which are conical in shape and bear one long opposite hollow marginal tentacle, the others as rudimentary bulbs with tentacle rudiments; all bulbs with ocelli. Developmental stages. Newly released medusa higher than wide, with small apical projection, exumbrella with numerous nematocysts; manubrium tubular, reaching 1/4 of subumbrellar height; mouth quadratic with 4 small lips; 4 ribbon-like radial canals and ring canal; 4 perradial marginal bulbs, each with an ocellus, one opposite pair of bulbs larger, conical, with a short tentacle on each. Colours: manubrium orange, red ocelli. Cnidome. Microbasic euryteles and desmonemes (hydroid and medusa). Distribution. Mediterranean Sea near Otranto, between Adriatic and Ionian Sea ( Boero et al. 1997b ; Gravili et al. 2008a ). Records in Salento. Rare at Otranto ( Denitto 1996 ; Boero et al. 1997b ; Miglietta et al. 2000 ). Remarks. The whole life cycle was examined in the present study. This species was originally identified as Codonorchis octaedrus by Boero et al. (1997b) . Schuchert (2007) disagreed with this identification, and described it as a new species, as it lacks the crucial feature of the genus Codonorchis , the gonads that extend to the subumbrella (this feature is unique and renders the genus very distinct). References. Boero et al. (1997b) , Gravili (2006) both as Codonorchis octaedrus ; Gravili et al. (2008a) , Schuchert (2007).