A reassessment of the Neotropical genus Pseudonannolene Silvestri, 1895: cladistic analysis, biogeography, and taxonomic review (Spirostreptida: Pseudonannolenidae) Author Iniesta, Luiz Felipe Moretti DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Bouzan, Rodrigo Salvador DEEF048E-97FB-4CCD-875F-5FA6184CA8AB&14A15A7F-730F-4D41-BDAC-D53514FAB85D Pós-graduação em Zoologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil. & Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com Author Brescovit, Antonio Domingos 14A15A7F-730F-4D41-BDAC-D53514FAB85D&D5B81D79-AFAE-47B1-8A6E-DAB448A24BCC Laboratório de Coleções Zoológicas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503 - 090, São Paulo, Brazil. rodrigobouzan@outlook.com&antonio.brescovit@butantan.gov.br text European Journal of Taxonomy 2023 2023-04-27 867 1 1 312 http://dx.doi.org/10.5852/ejt.2023.867.2109 journal article 57602 10.5852/ejt.2023.867.2109 1d2570da-e150-4d75-94d8-bfc5813062bc 2118-9773 7891021 8DEF295C-A8B1-4A6B-B873-B30949F64E07 Pseudonannolene pusilla Silvestri, 1895 Figs 111–112 , 176A , 178H , 186 Pseudonannolene pusilla Silvestri, 1895b: 7 , fig. 13. Pseudonannolene pusilla – Silvestri 1902: 23 . — Schubart 1958: 240 . — Viggiani 1973: 367 . — Jeekel 2004: 90 . — Golovatch et al. 2005: 279 . — Iniesta & Ferreira 2013a: 92 ; 2013c: 79 . Diagnosis Males of P. pusilla resemble those of P. morettii sp. nov. by having short coxae on the first leg-pair with a constriction at about midlength ( Fig. 112A ), but differing by the absence of long scattered setae on the mentum and stipes ( Fig. 176A ); solenomere short and subtriangular ( Fig. 112D ). Etymology Named after the Latin adjective ‘ pusillus ’ (feminine ‘ pusilla ’) = ‘very little’, ‘tiny’. Unspecified in the original description. Material examined (total: 5 ♂♂ , 2 ♀♀ , 3 immatures ) BRAZIL – Mato Grosso • 2 ♂♂ ; Pindaíba , Barra das Garças ; [ -15.881182 , -52.238738 ]; 337 m a.s.l. ; 19–31 Jan. 1998 ; M.E.V. Callefo leg.; IBSP 13390 • 3 ♂♂ , 2 ♀♀ , 3 immatures ; same collection data as for preceding; IBSP 13391 . Descriptive notes MEASUREMENTS . 55–57 body rings (1 apodous + telson). Males: body length 39.7 mm ; maximum midbody diameter 2.4 mm . Females: body length 43.1–44 mm ; maximum midbody diameter 3.7–4 mm . COLOR . Body color brownish grey; head and collum darker; prozonites anteriorly greyish; metazonites with a posterior band lighter; antennae and legs brownish. HEAD . Antennae long ( Fig. 111A ), just reaching back to end of ring 6 when extended dorsally; antennomeres elongated; relative antennomere lengths 1<2<3>4=5≈6>7. Mandibular cardo with ventral margin narrow. Ommatidial cluster well-developed, elliptical; ca 40 ommatidia in 5 rows. BODY RINGS . Collum with lateral lobes rounded, with ca 12 striae, strongly curved ectad ( Fig. 111A ). Very faintly constricted between prozonite and metazonite; prozonites smooth; metazonites laterally with transverse striae above ozopore in anterior body rings. Anterior sterna in midbody rings subrectangular, without transverse striae ( Fig. 176A ). FIRST LEG-PAIR OF MALES . Coxae ( cx ) short (less than half of remaining podomere lengths), subtriangular, with the base strongly arched and constricted medially, sparsely setose ( Fig. 112A ); prefemoral process ( prf ) less than half of prefemur, subcylindrical, densely setose up to its median region ( Fig. 112B ); remaining podomeres with setae along the mesal region. SECOND LEG-PAIR OF MALES . Coxa ( cx ) rounded; penis ( pn ) located at proximal region, rounded, not extended basally ( Fig. 112C ); prefemur compressed dorsoventrally; remaining podomeres setose. GONOPODS . Gonocoxa ( gcx ) elongated, twice longer than telopodite, with the base arched; antero-posteriorly flattened ( Fig. 112D–F ); with rows of papillae mesally. Seminal groove ( sg ) curved; arising medially on mesal cavity and terminating apically on the seminal apophysis ( sa ). Shoulder ( sh ) inconspicuous. Telopodite ( tp ) almost as wide as gcx ( Fig. 112D ); solenomere ( sl ) with small squamous region; apicomesal process ( amp ) subtriangular; ectal process absent; sa located at mesal portion, slightly visible apically in oral view ( Fig. 112F ). Internal branch ( ib ) subtriangular, narrow and foliaceous, surrounding basally tp as a shield; ib with setae along its entire margin nearly not exceeding apically seminal region of sl ( Fig. 112D–F ). VULVAE . As typical for the genus. Bursa subtriangular, glabrous ( Fig. 178H ); internal valve subtriangular, with its sides having the same length; operculum slightly curved ectad; external valve large, subtriangular. Distribution Known from Cerrado biome in the west region of Mato Grosso State , Brazil ; other records from the literature for the Brazilian states of Mato Grosso do Sul and Goiás , and region of the Chaco in Argentina and Paraguay ( Fig. 186 ). Comments The type material was described by Silvestri (1895b) and supposedly deposited at the Museo Regionale Scienze Naturali, Torino, Italy (MRSN) ( Viggiani 1973: 367 ), but was not found. Nevertheless, neartopotypes from Mato Grosso were examined ( Fig. 186 ). Other specimens from Brazil ( Goiás , Mato Grosso , and Mato Grosso do Sul ) and Paraguay ( Asunción ) were recorded by Silvestri (1902) .