A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species Author Wang, Xin-Cun Author Liu, Tie-Zhi Author Chen, Shuang-Lin Author Li, Yi Author Zhuang, Wen-Ying text MycoKeys 2019 60 45 67 http://dx.doi.org/10.3897/mycokeys.60.38186 journal article http://dx.doi.org/10.3897/mycokeys.60.38186 1314-4049-60-45 8003A6139B1050888874E5F93ABFF322 Helvella tianshanensis X.C. Wang & W.Y. Zhuang sp. nov. Figure 3 h-k Holotype. CHINA. Xinjiang Uygur Autonomous Region, Changji Hui Autonomous Prefecture, Jimsar County, 43°59'44"N , 89°10'31"E , alt. 1700 m, on the ground, 31 Jul 2003, W.Y. Zhuang & Y. Nong 4661, HMAS 86040. Etymology. The species epithet refers to the type locality of the fungus. Description. Apothecia stipitate, cupulate, margin undulate, involute, 2.5-3.5 cm high and 2-3 cm diam. when dry; hymenium greyish-brown, brown to dark brown, receptacle surface yellowish-brown to brown; stipe terete or flattened, buff, yellowish-brown, orange brown to brown, surface ribbed, 2-2.5 x 0.5-1.3 cm, typically fluted with rarely blunt ribs, ribs branching at the upper half of receptacle surface, reaching to the edge or ending 3-12 mm from the edge. Ectal excipulum of textura angularis , 120-150 µm thick, hyphae hyaline, outer cells 35-40 x 20-40 µm . Medullary excipulum of textura intricata , 350-600 µm thick, hyphae hyaline. Asci subcylindrical, tapering and with crozier at base, 8-spored, 240-275 x 12-24 µm . Paraphyses filiform, slightly enlarged at apical portion, septate, hyaline to light brown, 6-7.5 µm wide at apex and 3-4.5 µm below. Ascospores ellipsoidal, hyaline, smooth, uniguttulate, 17-21 x 11.5-13.5 µm , median 18.8 x 12.3 µm , Q = 1.35-1.7, median 1.51, n = 30. Additional specimen examined. CHINA. Xinjiang Uygur Autonomous Region, Urumqi City, Urumqi County, 43°28'47"N, 87°27'27"E, 12 Aug 1985, L. Fan & K. Tao 161, HMAS 88611. Notes. Helvella tianshanensis nested with H. calycina and H. costifera in Clade 1 ( Figure 1 ). These three species are hardly separated by gross morphology and anatomic structures. Helvella tianshanensis differs from H. calycina in 4 bp of Hsp90 (H022, epitype), 16 bp of ITS2 (HMAS 279704), 9 bp of LSU (H022) and 15 bp of TEF1 (H022); and it is different from H. costifera in 3 bp of Hsp90 (H298, epitype), 12 bp of ITS2 (HMAS 187120), 11 bp of LSU (H131) and 13 bp of TEF1 (H131). The two specimens of the new species are identical in Hsp90 and ITS2.