Resampling Bouché’s historical localities reveals three new species and helps identifying a new genus of earthworms (Oligochaeta, Hormogastridae and Lumbricidae) in Southeastern France Author Gérard, Sylvain Eco & Sols, INRAE, IRD, CIRAD, SupAgro Montpellier, Campus SupAgro, Bâtiment 12, 2 place Viala, F- 34060 Montpellier cedex 2 (France) Université de Montpellier, CNRS, EPHE, IRD, 1919, route de Mende, F- 34293 Montpellier cedex 5 (France) sylvain. gerard @ ens-paris-saclay. fr, sylvain. gerard 57 @ gmail. com (corresponding author) sylvain.gerard@ens-paris-saclay.fr & sylvain.gerard57@gmail.com Author Marchán, Daniel Fernández CEFE, Université de Montpellier, CNRS, EPHE, IRD, 1919, route de Mende, F- 34293 Montpellier cedex 5 (France) & Biodiversity, Ecology and Evolution Department, Faculty of Biology, Universidad Complutense de Madrid, Avenida de Séneca, 2 Ciudad Universitaria E- 28040 Madrid (Spain) danifermch@gmail.com Author Navarro, Alejandro Martinez Biodiversity, Ecology and Evolution Department, Faculty of Biology, Universidad Complutense de Madrid, Avenida de Séneca, 2 Ciudad Universitaria E- 28040 Madrid (Spain) alejma21@ucm.es Author Hedde, Mickaël Eco & Sols, INRAE, IRD, CIRAD, SupAgro Montpellier, SupAgro, Bâtiment 12, 2 place Viala, F- 34060 Montpellier cedex 2 (France) hedde@inrae.fr Author Decaëns, Thibaud CEFE, Université de Montpellier, CNRS, EPHE, IRD, 1919, route de Mende, F- 34293 Montpellier cedex 5 (France) thibaud.decaens@cefe.cnrs.fr text Zoosystema 2023 2023-12-05 45 23 749 768 https://sciencepress.mnhn.fr/sites/default/files/articles/pdf/zoosystema2023v45a23.pdf journal article 281520 10.5252/zoosystema2023v45a23 3f666a8b-4246-43ae-9afe-ce53163f4dc5 1638-9387 10275978 9115B736-A081-44B8-A5D6-DF5D45185BC3 Flabellodrilus luberonensis Gérard, Decaëns & Marchán , n. sp. ( Figs 3 ; 4 ) urn:lsid:zoobank.org:act: 7ACF81A1-FE52-4CB4-AC8D-9081EB9A5CB9 TYPE MATERIAL . — Holotype . France • 1 adult specimen; Provence-Alpes-Côte d’Azur , Vaucluse , D943, 84490 Saint-Saturnin-les-Apt, in the soil; 43°57’46”N , 5°20’13”E ; 434 m a.s.l. ; 25.XI.2021 ; T. Decaëns , D. F. Marchán leg.; MNHN ( BOLD Sample ID: DFM-1016 ). Paratypes . France • 2 adult specimens; same data as holotype; Eco & Sols ( BOLD Sample ID: DFM-1017, DFM-1020 ) • 2 adult specimens; same data as holotype; MNHN ( BOLD Sample ID: DFM-1018, DFM-1019 ). Other material . France • 8 sub-adult specimens; same data as holotype; Eco & Sols . ETYMOLOGY . — The species name is derived from the massif of Luberon where the type specimens were found. ECOLOGY . — F. luberonensis Gérard, Decaëns & Marchán , n. gen., n. sp. was found in a Mediterranean shrubland (garrigue) soil at low elevation ( 434 m a.s.l.). DISTRIBUTION. — F. luberonensis Gérard, Decaëns & Marchán , n. gen., n. sp. is only known from the type locality, near the city of Saint-Saturnin-les-Apt, between the massif of Luberon and the Vaucluse mountains in the Vaucluse department ( Provence-Alpes-Côte d’Azur , France ) ( Fig. 5 ). It is located in the Luberon Regional Natural Park (Parc naturel régional du Luberon). DESCRIPTION External morphology ( Fig. 3A, B ) Body shape cylindrical. Body pigmentation absent, mid-segmental dark dots in the cephalic region. Average body length after ethanol fixation: 80 mm (range: 70 to 100 mm ; n = 3 adult specimens; holotype : 100 mm ). Average body mass: 1.163 g (range: 0.855 to 1.398 g ; n = 3; holotype : 1.398 g ). Average diameter: 4.6 mm in the preclitellar region (range: 4.2 to 5.4 mm ; n = 5; holotype : 5.4 mm ), 5.8 mm in the clitellum (range: 5.2 to 6.8 mm ; n = 5; holotype : 6.8 mm ), 4.6 mm in the postclitellar region (range: 4.2 to 5.2 mm ; n = 5; holotype : 4.7 mm ). Average number of segments: 283 (range: 187 to353; n = 3; holotype : 309). Prostomium of closed epilobic type . Setae closely paired. Setal arrangement: aa:ab:bc:cd:dd = 14:2:8:1:32. Clitellum in XXXII-XLII (XLIII). Genital markings in XI-XII, randomly unpaired in XXVI-XXVIII, intraclitellar in XXXIV, in ab. Tubercula pubertatis: protruding band in XXXV-XL. Male pores: one pair in XV. Ovipores: one pair in 1/2 XIV, at 1 × ab dorsally from b. Spermathecal pores not visible. Nephridial pores not visible. FIG . 3. — Flabellodrilus luberonensis Gérard, Decaëns & Marchán , n. gen., n. sp. : A , external view of the anterior body; B , lateral view of the anterior body; C , dissection of the anterior body. Abbreviations: CL , clitellum, CR , crop, GM , genital marks, GZ , gizzard, IN , intestine, MP , male pore, NE , nephridia, SEP , septa, SP , permathecae, SV , seminal vesicles, TP , tubercula pubertatis. Photos: T. Decaëns. Scale bars: 5 mm. Internal anatomy ( Figs 3C ; 4 ) Septa : thickened from 5/6 to 9/10, otherwise membranous. Crop in XV-XVI. Muscular gizzard in XVII-XVIII followed by a membranous pouch in XIX, with an average size (width × length) of 3.55 × 2.10 mm . Calciferous glands: in X-XIV, forming a pair of spherical lobes in X. OEsophagus-intestine transition in XV.Typhlosole starts in XX, forming four folded lamellae. Hearts: in VI-XI. Excretory apparatus: one pair of holonephridia per segment, nephridial vesicles sigmoid with a sub-terminal constriction followed by a loop ( Fig. 4 ). Seminal vesicles: two pairs in XI-XII, large and lobulated. Spermathecae: two pairs in X-XI, intracoelomic, sessile and double ( Fig. 3C ). FIG . 4. — Pre-clitellar nephridia of Flabellodrilus luberonensis Gérard,Decaëns & Marchán , n. gen., n. sp. Scale bar: 1 mm. Photo: D. F. Marchán. REMARKS This species can be distinguished from its closest relative F. bartolii bartolii ( Bouché, 1970 ) n. comb. by the position of its clitellum and tubercula pubertatis ( Table 4 ) and by their remarkably high genetic distance (12.14% uncorrected pairwise COI distance).