Expression of green fluorescent protein defines a specific population of lamina II excitatory interneurons in the GRP :: eGFP mouse Author Watanabe, Kyohei Kanagawa Prefectural Museum of Natural History, 499 Iryuda, Odawara, Kanagawa 250 – 0031, Japan watanabe-k@nh‛kanagawa-museum‛jp text Bulletin of the Kanagawa Prefectural Museum (Natural Science) 2020 2020-03-31 49 29 66 http://dx.doi.org/10.5167/uzh-196963 journal article 296788 10.5167/uzh-196963 8a8398fc-02c0-4c25-985d-dced0f97f769 2189-6720 11266916 Agrothereutes minousubae Nakanishi, 1965 (Standard Japanese name: Minousuba-togarihimebachi) ( Figs 4 A-D) Agrothereutes minousubae Nakanishi, 1965: 456 . Material examined. JAPAN , KPM-NK 76583 , F, Tochigi Pref. , Nasushiobara City , Uwanohara , 21. VI. 2005 , E. Katayama leg. ; KPM-NK 76584 , M, Tochigi Pref. , Utsunomiya City , Yamato , 17. V. 2012 , T. Nakayama leg. ; NIAES , M, Tokyo , Kodaira , 5. V. 1969 , J. Minamikawa leg. ; NIAES , F, ditto, 22. V. 1965 ; NIAES , F, ditto, 1. VI. 1966 ; NIAES , F, ditto, 22. V. 1970 ; KPM-NK 76585–76587 , 1 F & 2 M, Kanagawa Pref. , Minamiashigara City , Tsukahara , 29. IV. 2019 , K. Watanabe leg. ; KPM-NK 76589–76591 , 3 M, ditto, 10. V. 2019 ; NIAES , 2 M, Chiba Pref. , Nagareyama , 15. V. 1976 , J. Minamikawa leg. ; NIAES , F, ditto, 29. V. 1976 ; KPM-NK 76592 , 76593 , F & M (det. R. Matsumoto ), Nara Pref. , Yamatokoriyama City , Yatacho , 1–2. V. 2016 , R. Matsumoto leg. ; KPM-NK 76594–76597 , 1 F & 3 M, Kagawa Pref. , Takamatsu City , Enza Town , Riverside of Koutougawa , 5. V. 2011 , K. Maeto & K. Watanabe leg. Remarks. This species had been reported from Kyushu (Nakanishi, 1965). In this study, I record this species from Honshu and Shikoku for the first time. This species quite resembles Ag. ramellaris in the body coloration and structures but can be clearly distinguished by the following character states: posterior transverse carina of propodeum complete (female and male of Ag. ramellaris ) or absent medially (female and male of Ag. minousubae ); area basalis of propodeum transversely elongate and slopping anteriorly (female and male of Ag. ramellaris ) or nearly quadrate and almost flat (female and male of Ag. minousubae ); wing base blackish brown (female and male of Ag. ramellaris ), yellowish brown (female of Ag. minousubae : Fig. 4B ) or yellow (male of Ag. minousubae ); posterior margin of T II (and usually also T III) with (female and male of Ag. minousubae : Figs 4A, C ) or without (female and male of Ag. ramellaris ) conspicuous reddish brown area; basal reddish area of hind femur sometimes indistinct (female of Ag. ramellaris ) or always distinct (female of Ag. minousubae : Fig. 4A ); yellow stripes of frontal and facial orbits separated by black area (male of Ag. ramellaris ) or united into a single stripe (male of Ag. minousubae : Fig. 4D ); apical margin of clypeus rounded (male of Ag. ramellaris ) or subtruncate (male of Ag. minousubae : Fig. 4D ); yellow spot of clypeus small or absent (male of Ag. ramellaris ) or large (male of Ag. minousubae : Fig. 4D ). The males of this species more or less vary in coloration, that is, subtegular ridge with or without a yellow spot, scutellum with or without a yellow spot and fore and mid coxae and trochanters each with or without yellow area. According to Nakanishi (1965), this species specifically attacked a single host, Pryeria sinica Moore, 1877 ( Lepidoptera : Zygaenidae ). I observed this species in Kanagawa prefecture that is the adult wasps flew around or walked on the hedge by Euonymus japonicus Thunb. ( Celastraceae ) damaged by the larvae of P. sinica . While all the males flew around the hedge, the female that I observed walked on the tree.