Chromosome numbers for the Italian flora: 12 Author Astuti, Giovanni Orto e Museo Botanico, Universita di Pisa, Via L. Ghini 13, 56126 Pisa, Italy giovanni.astuti@unipi.it Author Bartolucci, Fabrizio https://orcid.org/0000-0001-8199-6003 Centro Ricerche Floristiche dell'Appennino (Universita di Camerino - Parco Nazionale del Gran Sasso e Monti della Laga), San Colombo, 67021 Barisciano (L'Aquila), Italy Author Conti, Fabio https://orcid.org/0000-0001-7391-6691 Centro Ricerche Floristiche dell'Appennino (Universita di Camerino - Parco Nazionale del Gran Sasso e Monti della Laga), San Colombo, 67021 Barisciano (L'Aquila), Italy Author Cera, Beatrice Dipartimento di Scienze della Terra e dell'Ambiente, Universita di Pavia, Via S. Epifanio 14, 27100 Pavia, Italy Author Giaco, Antonio Dipartimento di Biologia, Universita di Pisa, Via Derna 1, 56126 Pisa, Italy Author Orsenigo, Simone https://orcid.org/0000-0003-1694-0585 Dipartimento di Scienze della Terra e dell'Ambiente, Universita di Pavia, Via S. Epifanio 14, 27100 Pavia, Italy Author Sandroni, Luca Dipartimento di Biologia, Universita di Pisa, Via Derna 1, 56126 Pisa, Italy Author Peruzzi, Lorenzo https://orcid.org/0000-0001-9008-273X Dipartimento di Biologia, Universita di Pisa, Via Derna 1, 56126 Pisa, Italy text Italian Botanist 2021 2021-12-15 12 123 131 http://dx.doi.org/10.3897/italianbotanist.12.79031 journal article http://dx.doi.org/10.3897/italianbotanist.12.79031 2531-4033-12-123 F6F9B2653E6958CB9365220DC9F0FBDA Allium permixtum Guss. (Amaryllidaceae) Chromosome number. 2 n = 24 (Fig. 1 ). Voucher specimen. Italy. Abruzzo. Rocca di Cambio ( L'Aquila ), Monte Ocre, vallone Canavine conca di Settacque, prati umidi, 1570 m (WGS84: 42.2471477N , 13.442932E ), 22 June 2005, F. Conti, F. Bartolucci, L. Bernardo, D. Iamonico, M. Latini, I. Londrillo, R. Lorenzetti, N. Ranalli, E. Pellegrini, L. Peruzzi, N. Ranalli, E. Scassellati, D. Tinti, V. Viscosi (APP15719, APP15721). Method. Squash preparations were made on root tips obtained from cultivated plants. Root tips were pre-treated with 0.4% colchicine for 4 h and then fixed in Carnoy solution for 1 h. After hydrolysis in 1N HCl at 60 °C, the tips were stained with leuco-basic fuchsine. Observations. Allium permixtum occurs in Italy and the Balkan Peninsula ( Anderson 1991 ; Conti 1995 ; Cheshmedzhiev and Marinov 2009 ; Brullo et al. 2010 ; Hallaci and Shuka 2013 ; Bartolucci et al. 2018 ). In Italy, this species was not recently confirmed in Sicily ( Brullo et al. 2010 ) and is very rare in Abruzzo, where it has been recorded only for some protected areas of the central Apennines, such as the National Park of Gran Sasso and Monti della Laga ( Conti 1998 ; Conti and Bartolucci 2016 ) and the National Park of Abruzzo, Lazio and Molise ( Conti 1995 ; Conti and Bartolucci 2015 ). The population studied here represents the first record for the Regional Park of Sirente-Velino (Abruzzo, central Italy). This is also the first chromosome count for the species in Italy ( Bedini and Peruzzi 2021 onwards), and it agrees with previous counts made for Greece and Bulgaria where both diploid and triploid cytotypes have been reported (2 n = 2 x = 16, 2 n = 3 x = 24) ( Tzanoudakis 1982 , 1986 ; Cheshmedzhiev and Marinov 2009 , in both sources under the name A. phthioticum ). F. Bartolucci, F. Conti Figure 1. Allium permixtum Guss. from Monte Ocre (Rocca di Cambio, L'Aquila ), 2 n = 24. Scale bar: 10 μm .