Latilactobacillus fragifolii sp. nov., isolated from leaves of a strawberry plant (Fragaria x ananassa)
Author
Legein, Marie
Author
Wittouck, Stijn
Author
Lebeer, Sarah
text
International Journal of Systematic and Evolutionary Microbiology
2022
005193
2022-01-21
72
1
1
9
http://dx.doi.org/10.1099/ijsem.0.005193
journal article
10.1099/ijsem.0.005193
cd46b772-abb8-4222-b765-7abff0f75896
1466-5034
PMC8895651
35060849
6048767
DESCRIPTION OF
LATILACTOBACILLUS FRAGIFOLII
SP. NOV.
Latilactobacillus fragifolii
(fra.gi.fo’li.i. L. neut. n.
fragum
strawberry plant; L. neut. n.
folium
leaf; N.L. gen. n.
fragifolii
of a strawberry leaf)
Cells of isolates AMBP158, AMBP159, AMBP162
T
, AMBP163, AMBP229, AMBP231, AMBP240, AMBP241, AMBP243, AMBP252, AMBP253, AMBP254 and AMBP256 are facultative heterofermentative, Gram-positive, catalase-positive, straight short rods of 0.8–1.6µm, which do not occur in long chains. They grow aerobically and anaerobically in MRS. Colonies grown aerobically on MRS agar at 30°C for 48 h are small, circular and white with a shiny surface. Acid is produced from L-arabinose, ribose,D-galactose, D-glucose,D-fructose,D-mannose,
N
-acetyl glucosamine, aesculin, salicin, cellobiose, sucrose, trehalose and gluconate. Acid is not produced from glycerol, erythritol, D-arabinose,D-xylose,L-xylose, adonitol, methyl β-xyloside, L-sorbose, rhamnose, dulcitol, inositol, mannitol, sorbitol, methyl α-D-mannopyranoside, methyl α-D-glucoside, amygdalin, arbutin, maltose, lactose, melibiose, inulin, melezitose, raffinose, starch, glycogen, xylitol, β-gentibiose, turanose, D-lyxose,D-tagatose,D-fucose,L-fucose,D-arabitol,L-arabitol and 2- or 5- keto-gluconate. Several phenotypic characteristics that are typical for this new species, such as catalase activity and utilization of L-arabinose and sucrose, could play a role in the adaptation of this species to the phyllosphere.
Table 4.
Comparative fatty acid compositions (%) of strain AMBP162T and type strains of the closely related species Strains: 1, AMBP162T; 2,
Latilactobacillus graminis
DSM 20719T; 3,
Latilactobacillus curvatus
DSM 20019T. –, Not detected.
| Fatty acid |
3 |
| 1 |
2
|
| Saturated: |
| C9:0 |
0.51 |
– |
– |
| C12:0 2OH |
0.63 |
– |
– |
| C14:0 |
5.09 |
0.84 |
0.92 |
| C16:0 |
12.10 |
8.01 |
8.81 |
| C18:0 |
2.96 |
2.24 |
2.66 |
| C19:0 iso |
1.96 |
1.93 |
2.00 |
| Unsaturated: |
|
C18:1ω9
c
|
58.38 |
80.27 |
73.86 |
| Ambiguous peaks: |
|
C16:1ω7
c
/C16:1ω6
c
|
4.61 |
– |
– |
|
C18:1ω7
c
/C18:1ω6
c
|
5.26 |
1.94 |
2.26 |
|
C19:0 cyclo ω10
c
/C19:1ω6
c
|
8.50 |
4.78 |
9.49 |
The
type
strain,
AMBP162
T
(=
LMG
32285
T
=
CECT 30357
T
), was isolated from the leaves of a strawberry plant growing in central
Portugal
.
The
genome size of strain
AMBP162
T
is 1.96
Mbp
and the DNA G+C content is 41 mol%
.