The genus Pheggomisetes Knirsch, 1923 (Coleoptera: Carabidae: Trechinae) in Serbia: taxonomy, morphology and molecular phylogeny Author Vrbica, Maja Author Petrović, Andjeljko Author Pantelić, Dejan Author Krmpot, Aleksandar J. Author Rabasović, Mihailo D. Author Pavlović, Danica Author Jovanić, Svetlana Author Guéorguiev, Borislav Author Goranov, Stoyan Author Vesović, Nikola Author Antić, Dragan Author Marković, Ðorđe Author Petković, Matija Author Stanisavljević, Ljubiša Author Ćurčić, Srećko text Zoological Journal of the Linnean Society 2018 2017-12-13 183 347 371 journal article 3384 10.1093/zoolinnean/zlx078 f5ee571f-a210-474d-882c-0b833b40e748 0024-4082 5714980 85900D92-A76D-4781-8829-CBED73A49334 PHEGGOMISETES GLOBICEPS CINIGLAVCENSIS ĆURČIĆ & VRBICA, SUBSP. NOV. ( FIGS 6 AND 7A–H ) Pheggomisetes globiceps ilandjievi: Gajović et al. (2011: 80) . Material examined: Holotype male labelled as follows: ‘Southeast Serbia , Stara Planina Mts., Propas Pit, 43°04′05.7″N 22°44′18.5″E , village of Činiglavci, 714 m a.s.l., near Pirot , 29.V-08.VII.2013 , from pitfall traps, leg. Ð. Marković & M . Petković’ (white label, printed)/‘ Holotypus Pheggomisetes globiceps ciniglavcensis subsp. nov. Ćurčić, Vrbica & Guéorguiev det. 2016’ (red label, printed) ( IZFB ). Paratypes : 29 males and 20 females , same data as for holotype ( IZFB ). All paratypes are labelled with white printed locality labels and with red printed labels ‘ Paratypus Pheggomisetes globiceps ciniglavcensis subsp. nov. Ćurčić, Vrbica & Guéorguiev det. 2016’. Description: TL R 6.15–6.825 mm (M 6.46 mm ) (HT 6.525 mm ). Head oval, HL/HW R 1.19–1.355 (M 1.275) (HT 1.19), widest somewhat before its mid part, scarcely wider than pronotum ( Fig. 6 ). Frontal furrows almost reaching mid head level, deeply impressed anteriorly and sigmoidally curved. Neck narrow, HW/NW R 2.17–2.61 (M 2.405) (HT 2.50). Antennae long, longer (in males) or slightly shorter (in females) than TL. Pronotum widest somewhat after the anterior third, almost as long as wide ( Fig. 6 ). Anterior pronotal margin slightly concave, shorter than pronotal base. Lateral pronotal margins rounded anteriorly and slightly concave posteriorly. Pronotal base very slightly concave in the middle. Fore pronotal angles obtuse, rounded. Hind pronotal angles acute, almost right. Male abdominal sternite IX (urite) subtriangular, slightly elongate, somewhat longer than aedeagus ( Fig. 7C, G ). Apophysis narrow, gradually narrowing distally. Both gonocoxites IX and gonosubcoxites IX as presented in Figure 7D, H . Gonocoxites IX slightly elongate, somewhat curved, apically rounded, basally completely jointed with massive gonosubcoxites IX ( Fig. 7D, H ). Chaetotaxy. Frons with five to seven setae (HT – 6–7) on each side. Pronotum with normal chaetotaxy (two pairs of setae). Six to eight setae on third interstria (HT – 6–7) on each elytron ( Fig. 6 ). Elytral umbilicate series: First three humeral setae close to marginal gutter, fourth being somewhat farther from the gutter, distance between umbilicate pores 2 and 3 shortest, distance between pores 1 and 2 is approximately the same as between pores 3 and 4; median series at around the middle of the elytra, two setae being somewhat distanced from marginal gutter, distance between pores 5 and 6 about as long as distance between pores 2 and 3; apical series: setae 7 and 8 being somewhat distanced from marginal gutter, distance between pores 7 and 8 longer than distance between pores 3 and 4 ( Fig. 6 ). Figure 6. Pheggomisetes globiceps ciniglavcensis subsp. nov. from the Propas Pit, village of Činiglavci (near Pirot), Stara Planina Mts., Southeast Serbia. Holotype male, habitus (dorsal view). Scale = 5.0 mm. Elytra relatively long, oval, convex, widest somewhat after the mid level, EL/EW R 1.53–1.73 (M 1.64) (HT 1.68). Elytral base slightly wider than pronotum (BW/ PW R 0.85–1.10, M 1.03, HT 1.00) ( Fig. 6 ). Humeral angles obtuse, rounded and relatively elevated. Elytral apex rounded. Legs and claws long and thin ( Fig. 6 ). Median lobe of the aedeagus curved, slightly convex dorsally around the basal fourth, with a rounded apex ( Fig. 7A, B, E, F ). Basal bulb small, rounded. Parameres with three setae each, of which two are apically positioned. Triangular gutter-shaped copulatory piece covered with numerous thorns ( Fig. 7B, F ), gradually narrowed apically in dorsal aspect. Differential diagnosis: The known subspecies of P. globiceps differ in shape of the head, length and depth of the frontal furrows, shape of the hind pronotal angles, lateral margins of the head and pronotum, the HL/PL and shape of the humeral angles ( Guéorguiev, 1964 ). Some new characters should also be taken into account in separating Pheggomisetes taxa (e.g. TL, HL, HL/HW, HL/AL, HL/TL, HW/ NW, HW/PW, HW/EW, AL, AL/TL, PL, PL/PW, PL/ TL, PaW, PbW, PW, PW/EW, PWP, EW, EL/EW, BW/ PW, elytral umbilicate series position, and aedeagus and copulatory piece shapes) ( Tables 2 and 3 ; Supporting Information, Table S1 ). The new subspecies is compared here with the morphologically and geographically closest subspecies of P. globiceps and the nominotypical subspecies. The former are P. globiceps ilandjievi ( Figs 10 , 11A–D ) and P. globiceps ninae comb. & stat. nov. (with the head elongately ovoid, lateral margins of the head moderately rounded, head slightly rounded both anteriorly and posteriorly as well, acute/right posterior pronotal angles, pronotum basally constricted, head slightly broader than pronotum and humeral angles slightly elevated) ( Guéorguiev, 1964 ; Ćurčić et al. , 2004 ; this article). Pheggomisetes globiceps ciniglavcensis subsp. nov. differs from P. globiceps ilandjievi in having a smaller value of TL M (6.46 vs. 6.60 mm ), different shape of the head (widest at 2/5 of its length vs. widest slightly before the middle), a smaller value of HL/HW M (1.275 vs. 1.32), a greater value of AL M (6.775 vs. 6.64 mm ), a greater value of AL/TL M (1.05 vs. 1.01), different shape of the lateral pronotal margins (less rounded anteriorly, more concave posteriorly vs. more rounded anteriorly, less concave posteriorly), greater values of PaW (R 0.63–0.68, M 0.66 vs. R 0.53–0.595 mm , M 0.57 mm ), a greater value of PbW M (0.83 vs. 0.75 mm ), a greater value of PaW/PW M (0.64 vs. 0.57), different shape of the humeral angles (less rounded and less elevated vs. more rounded and more elevated), a smaller value of EL/EW M (1.64 vs. 1.83), a greater value of BW/PW M (1.03 vs. 0.95), different shape of the median lobe (slightly convex dorsally around the basal fourth, with a narrower apex in dorsal view vs. not convex dorsally around the basal fourth, with a wider apex in dorsal view) and different size of the basal bulb (smaller vs. bigger) (Supporting Information, Table S1 ) ( Guéorguiev, 1964 ; this study). Figure 7. Pheggomisetes globiceps ciniglavcensis subsp. nov. from the Propas Pit, village of Činiglavci (near Pirot), Stara Planina Mts., Southeast Serbia. Bright-field (A–D) and TPEF (E–H) microscopy images. A, E, holotype male, aedeagus (lateral view). B, F, holotype male, aedeagus (dorsal view). C, G, holotype male, abdominal sternite IX (urite). D, H, paratype female, gonocoxites IX and gonosubcoxites IX. Scales = 0.1 mm. Pheggomisetes globiceps ciniglavcensis subsp. nov. differs from P. globiceps ninae comb. & stat. nov. in having a greater value of TL M (6.46 vs. 6.295 mm ), a smaller value of HL/HW M (1.275 vs. 1.30), a greater value of FL M (0.68 vs. 0.63 mm ), greater values of AL M (6.775, males 6.84, females 6.50 vs. 6.525 mm , males 6.56 mm , females 6.30 mm ), a greater value of HW/NW M (2.405 vs. 2.26), a greater value of EL M (3.50 vs. 3.36 mm ), a greater value of EW M (2.135 vs. 1.99 mm ), a smaller value of EL/EW M (1.64 vs. 1.69), a greater value of BW/PW M (1.03 vs. 0.91), different shape of the median lobe (narrower, somewhat more curved basally, then regularly curved, slightly convex dorsally around the basal fourth, with a narrow anterior part in dorsal view vs. thicker, regularly curved, somewhat convex dorsally in the middle, with a wide anterior part in dorsal view) and a different number of parameral setae (three, two of them apical vs. five, three of them apical) (Supporting Information, Table S1 ) ( Ćurčić et al. , 2004 ; this study). Pheggomisetes globiceps ciniglavcensis subsp. nov. differs from P. globiceps globiceps ( Figs 12 , 13A–D ) in having different shape of the head (widest at the anterior 2/5 of its length vs. widest somewhat after the middle), a greater value of HW/NW M (2.405 vs. 2.24), greater values of AL M (6.775, males 6.84, females 6.50 vs. 6.585 mm , males 6.775 mm , females 6.30 mm ), a smaller value of HL/PL M (1.51 vs. 1.63), a greater value of PL/PW M (0.91 vs. 0.88), a greater value of EW M (2.135 vs. 2.02 mm ), a smaller value of EL/EW M (1.64 vs. 1.725), a greater value of BW/PW M (1.03 vs. 0.885), different shape of the median lobe (narrower, elongate, with more elongate basal bulb vs. wider, stout, especially basally, with a short stout basal bulb) and different shape of the copulatory piece in dorsal aspect (gradually narrowing towards the apex vs. wide at the basal 3/5 and markedly narrowed at the apical 2/5) (Supporting Information, Table S1 ) ( Guéorguiev, 1964 ; this study). Variability: The number of setae on both frons (five to seven on each side) and elytra can vary (six to eight on each side). Etymology: The subspecies is named after the village of Činiglavci, in which the type locality is situated. Distribution: It lives solely in the Propas Pit in the village of Činiglavci (near Pirot ) in the Stara Planina Mountains of Southeast Serbia . Remarks: The new subspecies was originally treated as P. globiceps ilandjievi by Gajović et al. (2011), who collected a sample several years ago.