A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles ronaldzunigai Arias-Penna, sp. nov. Figs 193 , 194 Female. Body length 1.86 mm , antenna length 2.07 mm , fore wing length 2.22 mm . Type material. Holotype : COSTA RICA • 1♀ ; 08-SRNP-55003, DHJPAR0020565; Area de Conservacion Guanacaste , Guanacaste , Sector Mundo Nuevo , Vado Ficus ; dry-rain intergrade forest; 375 m ; 10.77090 , -85.42455 ; 04.i.2008 ; Mariano Pereira leg.; caterpillar collected in fourth instar; very small dark cocoons adhered lightly to cuticle, jumbled, cocoons formed on 14.i.2008 ; adult parasitoids emerged on 18.i.2008 ; ( CNC ) . Paratypes . • 22 ( 2♀ , 2♂ ) ( 12♀ , 6♂ ); 08-SRNP-55003, DHJPAR0020565; same data as for holotype; ( CNC ) . Diagnosis. Dorsal carina delimiting a dorsal furrow on propleuron absent ( Figs 193J , 194I ), surface of metasternum convex, precoxal groove deep with lineate sculpture ( Figs 193A, J , 194A, I ), fore wing with vein 1 cu-a curved, r vein curved ( Figs 193L , 194K ), dorsal outer depression on hind coxa present ( Figs 193A, K , 194A, J ), inner margin of eyes diverging slightly at antennal sockets ( Figs 193C , 194B ), petiole on T1 finely sculptured ( Figs 193H , 194G, H ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Figs 193H, I , 194G, H ). Figure 193. Glyptapanteles ronaldzunigai sp. nov. female 08-SRNP-55003 DHJPAR0020565 A Habitus B-D Head B Dorsal view C Frontal view D Lateral view E Genitalia: hypopygium, ovipositor, ovipositor sheaths, lateral view F Mesonotum, dorsal view G Scutellum, metanotum, propodeum, dorsal view H T1-3, dorsal view I, K Metasoma I Dorsal view K Lateral view J Mesosoma, lateral view L Fore and hind wings. Figure 194. Glyptapanteles ronaldzunigai sp. nov. male 08-SRNP-55003 DHJPAR0020565 A Habitus B-D Head B Frontal view C Lateral view D Dorsal view E Mesonotum, dorsal view F Scutellum, metanotum, propodeum, dorsal view G T1-3, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view K Fore and hind wings. Coloration ( Fig. 193 A-L ). General body coloration black except yellow-brown scape distally brown; brown pedicel distally yellow-brown; all antennal flagellomeres dark brown on both sides; labrum, mandibles, and tegulae yellow-brown; glossa, maxillary and labial palps ivory/pale yellow. Eyes black and ocelli silver/reddish (in preserved specimen). Fore and middle legs yellow except brown coxae and claws; hind legs yellow-brown except black coxae distally yellow-brown, femora distally brown, distal half of tibiae, and tarsomeres brown, although proximally basitarsus with a small yellow band. Petiole on T1 dark brown, contours darkened and sublateral areas yellow-brown; T2 with median and adjacent areas brown, together forming a rectangle well-defined, and lateral ends yellow; T3 almost completely brown; T4 and beyond completely brown; distally each tergum with a whitish transparent band. In lateral view, T1-3 yellow; T4 and beyond brown. S1-3 yellow; S4 and beyond brown. Description. Head ( Fig. 193 A-D ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres longer than wide (0.17:0.06, 0.17:0.06, 0.16:0.06), distal antennal flagellomere longer than penultimate (0.10:0.05, 0.08:0.05), antenna longer than body (2.07, 1.86); antennal scrobes-frons shallow. Face flat or nearly so, with dense and finely punctate, interspaces smooth and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.09, 0.13). Malar suture absent or difficult to see. Median area between lateral ocelli slightly depressed. Vertex laterally rounded and dorsally wide. Mesosoma ( Fig. 193A, F, G, J ). Mesosoma dorsoventrally convex. Mesoscutum 1/4 distal with a central dent, punctation distinct throughout, interspaces smooth. Scutellum triangular, apex sloped and fused with BS , scutellar punctation distinct throughout, in profile scutellum slightly convex, but on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM ; ATS demilune with complete undulate/reticulate carinae; dorsal ATS groove with carinae only proximally. Transscutal articulation with small and heterogeneous foveae; area just behind transscutal articulation depressed centrally, smooth and shiny. Metanotum with BM wider than PFM (clearly differentiated); MPM circular and bisected by a median longitudinal carina; AFM without setiferous lobes and not as well delineated as PFM ; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum without median longitudinal carina, proximal half curved with medium-sized sculpture and distal half slightly rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle without distal carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a well-defined smooth band; central area of pronotum smooth, but both dorsal and ventral furrows with short parallel carinae. Propleuron rugose and dorsally without a carina. Metasternum convex. Contour of mesopleuron convex; precoxal groove deep and with transverse lineate sculpture; epicnemial ridge convex, teardrop-shaped. Legs. Ventral margin of fore telotarsus excavated with conspicuous curved seta over this excavation, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.11, 0.05). Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.21, 0.16), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.12, 0.10). Wings ( Fig. 193L ). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein straight, distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with a small smooth area; vein 2CUa absent and vein 2CUb spectral; vein 2 cu-a absent; vein 2-1A present only proximally as tubular vein; tubular vein 1 cu-a curved and complete, but junction with 1-1A vein spectral. Hind wing with vannal lobe wide, subdistally concave and subproximally straightened, and setae evenly scattered in the margin. Metasoma ( Fig. 193A, E, H, I, K ). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, virtually parallel-sided over most of length, but narrowing over distal 1/3 (length 0.29, maximum width 0.14, minimum width 0.07), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.11, length T2 0.11), edges of median area polished and lateral grooves deep, median area broader than long (length 0.11, maximum width 0.17, minimum width 0.06); T2 with scattered pubescence only distally. T3 longer than T2 (0.19, 0.11) and with scattered pubescence throughout. Pubescence on hypopygium scattered. Cocoons. Oval cocoons with silk fibers evenly smooth. Untidy cocoons adhered lightly to cuticle. Comment. Specimens short and stout ( Fig. 193A ). Male ( Fig. 194 A-K ). Similar in coloration and shape to female. Etymology. Ronald Zuniga is a specialist in Hymenoptera who works at the Instituto Nacional de Biodiversidad ( INBio ), Santo Domingo de Heredia , Costa Rica . Distribution. The parasitized caterpillar was collected in Costa Rica , ACG, Sector Mundo Nuevo (Vado Ficus ), during January 2008 at 375 m in dry-rain intergrade forest. Biology. The lifestyle of this parasitoid species is gregarious. Host. Macaria nundinata Guenee ( Geometridae : Ennominae ) feeding on Dalea carthagenensis ( Fabaceae ). Caterpillar was collected in fourth instar.