A new study of Nagrajomyces: with two new species proposed and taxonomic status inferred by phylogenetic methods Author Zhuo, Lan College of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, China Author Guo, Mei-Jun College of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, China Author Wang, Qiu-Tong https://orcid.org/0000-0002-7548-5693 College of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, China Author Zhou, Hao https://orcid.org/0000-0002-4869-2187 College of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, China Author Piepenbring, Meike https://orcid.org/0000-0002-7043-5769 Mycology Research Group, Faculty of Biological Sciences, Goethe University Frankfurt am Main, Max-von-Laue-Str. 13, D- 60438 Frankfurt am Main, Germany Author Hou, Cheng-Lin College of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, China chenglin-hou@cnu.edu.cn text MycoKeys 2022 2022-10-25 93 131 148 http://dx.doi.org/10.3897/mycokeys.93.93712 journal article http://dx.doi.org/10.3897/mycokeys.93.93712 1314-4049-93-131 D6D5DF7DBC395F608A853F3004D97AAB Nagrajomyces fusiformis C. L. Hou & L. Zhuo sp. nov. Figs 2 , 3 Etymology. The epithet Nagrajomyces fusiformis refers to fusoid conidia. Type . China , Yunnan province , Lijiang , Yulong , 26°40'55"N , 99°54'01"E , alt. 2762 m , on dying twigs of Rhododendron vellereum Hutch. ex Tagg. , 20 June 2021 , coll. C.L. Hou, M.J . Guo, H . Zhou ( holotype CAF 800050) . Diagnosis. This new species differs from N. dictyosporus and N. laojunshanensis by fusoid to elongate-fusoid conidia with pointed ends, usually 1-septate and smaller. Figure 2. Micrographs of Nagrajomyces fusiformis (holotype CAF 800050) on twigs of Rhododendron vellereum A, B conidiomata on a dying twig C vertical section of a conidioma D conidiophores and conidia at diverse developmental stages E-H conidia with appendages. Scale bars: 2 mm ( A ); 200 µm ( B ); 100 µm ( C ); 10 µm ( D-H ). Figure 3. Nagrajomyces fusiformis (holotype CAF 800050) A vertical section of a conidioma B conidiophores and conidia C conidia with appendages. Scale bars: 100 µm ( A ); 10 µm ( B ); 5 µm ( C ). Description. Conidiomata solitary, pycnidial, irregularly plurilocular, subepidermal in origin, immersed at first, then becoming erumpent through the periderm of the host, 545-554 μm diameter, 520-546 μm high, peridium dark brown, 47.0-67.5 μm thick. Conidiophores ampulliform, smooth, hyaline, multiguttulate, 12-29 x 2.0-3.5 μm (x̄ = 19 x 3 μm , n = 20). Conidia fusoid to elongate-fusoid, 1-septate, cells equal, smooth, hyaline to pale brown, 13.5-19.0 x 3-4 μm (x̄ = 16.5 x 3.5 μm , n = 20), with a whip-like appendage at the tip of each conidium, 30-77 μm (x̄ = 51 μm , n = 20) in length (Fig. 4 ). Sexual morph not observed. Additional specimen examined. China, Yunnan Province, Lijiang, Laojunshan, 26°37'56"N , 99°43'30"E , alt. 3873 m, on dying twigs of Rhododendron vellereum , 20 June 2021, coll. C.L. Hou, M.J. Guo, H. Zhou (BJTC 1773). Notes. Nagragomyces fusiformis differs from other species of Nagrajomyces by narrower and 1-septate conidia.