Novomicrothelia pandanicola sp. nov., a non-lichenized Trypetheliaceae species from Pandanus Author Zhang, Sheng-Nan Guizhou Institute of Biotechnology, Guizhou Academy of Agricultural Science, Guiyang 550006, Guizhou, P. R. China. & Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Science, Guiyang 550006, Guizhou, P. R. China. & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. & Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Author Hyde, Kevin D. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. Author Jones, E. B. Gareth Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Kingdom of Saudi Arabia. Author Cheewangkoon, Ratchadawan Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. Author Boonmee, Saranyaphat Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. Author Doilom, Mingkwan Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. Author Mapook, Ausana Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. Author Liu, Jian-Kui Guizhou Institute of Biotechnology, Guizhou Academy of Agricultural Science, Guiyang 550006, Guizhou, P. R. China. & Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Science, Guiyang 550006, Guizhou, P. R. China. text Phytotaxa 2017 2017-09-19 321 3 254 264 http://dx.doi.org/10.11646/phytotaxa.321.3.3 journal article 302356 10.11646/phytotaxa.321.3.3 dac5274b-b19f-4ab5-a634-1aaefa9be78e 1179-3163 13697696 Novomicrothelia pandanicola S.N. Zhang & K.D. Hyde , sp. nov. Index Fungorum number : IF 553176; Facesoffungi number : FoF 03289; Figure 2 . Family : Trypetheliaceae Etymology : Name reflects to the host Pandanus tectorius , from which the holotype was collected. Holotype : MFLU 17-0753 Saprobic on the bark of Pandanus tectorius , with a tropical distribution. Sexual morph : Thallus ecorticate, non-lichenized, some part bordered by dark prothallus lines. Ascomata 490–990 μm diam., 190–200 μm high, solitary or irregularly confluent, immersed to erumpent, subglobose to flask-shaped, covered with black pseudoclypeus, in vertical section 183–305 μm diam., 177–248 μm high. Ostioles apical or rarely eccentric. Peridium 18–29 μm wide, with 5–6 layers of compressed cells of textura angularis , coriaceous, partly carbonaceous, dimidiate, comprising a mixture of host cells and brown fungal hyphae, K+ (olivaceous within 10% KOH solution). Hamathecium up to 1.5 μm wide, hyaline, filamentous, sparsely septate, branched and anastomosing pseudoparaphyses, embedded in a gelatinous matrix. Asci 75–137 × 12–20 μm, ( x = 93.8 × 14.5 μm, n = 18), 8-spored, bitunicate, narrowly clavate to subcylindrical, pedicellate, occasionally constricted at the upper part, apically rounded, with a small ocular chamber, up to 1.5 μm high. Ascospores 16–32 × 5–10 μm ( x = 24 × 7.3 μm, n = 30), hyaline, becoming brown with age, partly overlapping or biseriate, ellipsoid to obovoid or fusiform, verruculose, muriform and elongated, with 3–7 transverse septa, and 1–4 longitudinal septa in all cells and rarely in end cells, sometimes strongly constricted at the middle septum, straight or slightly curved, wall smooth, surrounded by an irregular, gelatinous sheath, observed more clearly when mounted in Indian ink. Asexual morph : Undetermined. FIGURE 2. Novomicrothelia pandanicola (MFLU 17-0753, holotype). a–b Appearance of thallus and ascomata on host surface. c Close up ascoma on host surface. d Vertical section of ascoma. e–f Structure of peridium, f Peridium within 10% KOH solution. g Asci with paraphysoids. h–i Asci. j Trabeculate pseudoparaphyses. k Ascus apex with small ocular chamber. l–p Immature Ascospores, n Ascospore surrounded by an irregular gelatinous sheath in Indian ink, q Mature ascospore. Scale bars: b = 500 μm, c–d = 50 μm, e–f = 10 μm, g–i = 20 μm, j–q = 10 μm. Culture characteristics :—Ascospores germinating on PDA within 48 hours at room temperature in natural light. Germ tubes produced from each end. Colonies growing well on both PDA and MEA media, and attaining a diameter of 1.5 cm on PDA after two months at room temperature, slow growing, obverse olive to grey-green or light grey-green, tufted colony center elevated, reverse dark green ( Figure 3 ). Mycelium 2–3 μm wide, hyaline to pale brown, aerial, septate, ornamented, branched and anastomosing. Chlamydospores 7.7–43 × 5−11.5 (x = 16.8 × 7.3 μm, n = 20), produced from mycelia, initially subglobose with 1 cell, hyaline, becoming 1–5 septate, pale brown to dark brown. Material examined :— THAILAND , Chanthaburi , Ao Khung Kraben, on dead bark of Pandanus tectorius ( Pandanaceae R.Br. ), 7 July 2016 , M. Doilom SN-T-04 (MFLU 17-0753, holotype ); ex-type culture MFLUCC 16- 1382 = GZCC 17-0004. (HKAS 96378, isotype ).