Four new species of Macrostomus Wiedemann and a checklist of Empididae s. str (Diptera) from Peru Author Rafael, José Albertino 0000-0002-0170-0514 Instituto Nacional de Pesquisas da Amazônia, Coordenação de Biodiversidade, Manaus, Amazonas, Brazil. jarafael @ inpa. gov. br; https: // orcid. org / 0000 - 0002 - 0170 - 0514 jarafael@inpa.gov.br Author Marques, Dayse Willkenia A. 0000-0001-7260-5760 Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Entomologia, Manaus, Amazonas, Brazil. willkenia @ gmail. com; https: // orcid. org / 0000 - 0001 - 7260 - 5760 willkenia@gmail.com text Zootaxa 2021 2021-06-08 4981 3 506 530 journal article 5899 10.11646/zootaxa.4981.3.5 fce50f60-b834-424f-9a19-cf4601ffd2a3 1175-5326 4921345 3F94E1C8-2F49-4B85-86E3-48341F17A358 Macrostomus lineatus Rafael & Cumming ( Figs 12A–Q ) Macrostomus lineatus Rafael & Cumming, 2006: 54 . Type locality: Ecuador , Napo-Pastaza , Puyo . M. lineatus : Yang et al ., 2007: 145 (cat.). Diagnosis. Male. Postcranium black with no pruinosity on upper third; thorax and legs mainly yellow ( Fig. 12A ); scutum with a median black longitudinal stripe between dorsocentral setae ( Fig. 12B ); postsutural supra-alar seta absent; hind femur distinctly clavate ( Fig. 12A ); cell dm rather truncate ( Fig. 12B ); tergite 8 ( Figs 12C, D ) with bifid distal projection and deep basal cleft; sternite 8 ( Fig. 12E ) with wide U-shaped basal sinus; epandrium ( Fig. 12F ) with posterodorsal lobe wider, slightly higher than anterodorsal lobe, with spiniform setae internally ( Fig. 12G ); anterior cercus ( Fig. 12H ) distinctly setose, with wide mesial process ( Fig. 12L ); cercal bridge ( Figs 12H–J, L ) as a well sclerotized, wide central plate; median cercus elevated, with slender setae; posterior cercus as a long, thin and curved downwards branch, inwardly and forward directed with yellow digitiform setae at apex, internally and basally with two shorter protuberances, one curved with long yellow setae at apex and the other, near base, with straight, small yellow setae at apex ( Fig. 12K ); ventral process of cercus with no distinct setae, acute distally ( Fig. 12J ); dorsal and ventral hypoproctal lobes distinct, but small, setose ( Fig. 12L ); hypandrium ( Fig. 12M ) with few posterior setae on basal third, with a long posterior trilamellar projection medially and small longitudinal grooves at apex; ejaculatory apodeme tetralamellar; phallus shorter than hypandrium length ( Fig. 12N ). Female. Tergite 8 with wide distal sinus ( Fig. 12P ); sternite 8 with perpendicular median cleft laterally and a small, sclerotized area within the cleft ( Figs 12O, P ); genital fork ( Fig. 12Q ) with small cleft distally, with genital chamber membranous. Geographical records. Ecuador , Peru . Material examined. Listed in Rafael & Cumming (2006) . Remarks. This species was described based on a couple of specimens from Ecuador (Napo-Pastaza) and one female specimen from Peru (Avispas, Madre de Dios ). The female specimen from Peru needs to be associated with a male from the same locality in order to ensure that it is conspecific with M. lineatus .