Four new species of Macrostomus Wiedemann and a checklist of Empididae s. str (Diptera) from Peru Author Rafael, José Albertino 0000-0002-0170-0514 Instituto Nacional de Pesquisas da Amazônia, Coordenação de Biodiversidade, Manaus, Amazonas, Brazil. jarafael @ inpa. gov. br; https: // orcid. org / 0000 - 0002 - 0170 - 0514 jarafael@inpa.gov.br Author Marques, Dayse Willkenia A. 0000-0001-7260-5760 Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Entomologia, Manaus, Amazonas, Brazil. willkenia @ gmail. com; https: // orcid. org / 0000 - 0001 - 7260 - 5760 willkenia@gmail.com text Zootaxa 2021 2021-06-08 4981 3 506 530 journal article 5899 10.11646/zootaxa.4981.3.5 fce50f60-b834-424f-9a19-cf4601ffd2a3 1175-5326 4921345 3F94E1C8-2F49-4B85-86E3-48341F17A358 Macrostomus pictipennis (Bezzi) ( Figs 5A–F ) Rhamphomyia pictipennis Bezzi, 1909: 330 . Type locality: Bolivia , La Paz , Mapiri , San Carlos . Macrostomus pictipennis ; Rafael & Cumming, 2004: 446 ; Yang et al ., 2007: 145 (cat.); Rafael & Cumming, 2010: 619 (redescr.); Rafael & Marques, 2019: 273 , 274 (cit.). Diagnosis. This species is characterized by male and female with four pairs of dorsocentral setae; wing with brown to black infuscation at level of crossveins r-m and dm-m ( Fig. 5A ). Male. Tergite 8 ( Fig. 5C ) elongated with distal protuberances; epandrium ( Fig. 5C ) slightly swollen with strong posterodorsal lobe longer than anterodorsal lobe; hypandrium ( Fig. 5D ) with slender posterior setae; ejaculatory apodeme trilamellar, dorsal lamella absent; phallus same length as hypandrium. Female. Pinnate setae dorsally and ventrally on mid femur and a few short pinnate setae on basal fourth of mid tibia ventrally ( Fig. 5E ); hind femur and tibia with pinnate setae dorsally and ventrally ( Fig. 5F ). Geographical records. Bolivia , Peru . Material examined. PERU : Cuzco , Quincemil , 13–31.viii.1962 , L. Pena , 700 m ( 1♀ INPA ) . FIGURE 5 . Macrostomus pictipennis ( Bezzi, 1909 ) from Cuzco, Peru. ♂ (A–D) . A . Wing; B . Tergite 8, dorsal view; C . Apex of abdomen, lateral view; D . Hypandrium, lateral view. ♀ (E–F) . E . Mid leg, posterior view; F . Hind leg, posterior view. Scale bars, figure A = 1 mm; B–D = 0.2 mm; E–F = 0.5 mm (figures after Rafael & Cumming (2010)) . Abbreviations: dm–m —discal medial crossvein; epand , epandrium; r–m —radial–medial crossvein; t , tergite. Remarks. This species was originally described on the basis of a single female specimen from La Paz , Bolivia and presently it is in poor condition. It was associated with specimens from Quincemil, Cuzco , Peru by Rafael & Cumming (2010) . No male from the type-locality was studied and this is essential to be secure that the Peruvian specimens are conspecific with Bolivian specimens of M. pictipennis .