New records of 24 ciliate species (Protozoa, Ciliophora) collected in South Korea Author Kim, Ji Hye Natural Science Research Institute, Gangneung-Wonju National University, Gangneung, Republic of Korea Author Jung, Ji Hye Moon and Jae-Ho text Journal of Species Research 2018 7 4 291 314 journal article 10.12651/JSR.2018.7.4.291 2713-8615 13163147 11. Urostyla grandis grandis Ehrenberg, 1830 ( Fig. 11 ) Material examined. Freshwater with debris collected from Gyeongpocheon River , Unjeong-dong , Gangneung-si , Gangwon-do , Korea ( 37°47 ʹ 20 ʺ N , 128°54 ʹ 34 ʺ E ) on January 4, 2018 . Diagnosis. Body size about 200 × 95 μm after protargol impregnation; body broadly ellipsoidal; flexible; cytoplasm colorless; yellowish cortical granules; numerous ellipsoidal to globular macronuclear nodules; 46- 51 adoral membranelles; bicorona frontal rows; 4-6 left marginal rows; 5 or 6 right marginal rows; 8-13 transverse cirri; 4 dorsal kineties; marginal cirral row originated from intrakinetal cirral anlage. Distribution. Cosmopolitan. Remarks. A Korean population of U. grandis was previously described without morphogenesis ( Shin, 1994 ; Berger, 2006 ). Recently, Paiva et al . (2016) established a new subspecies U. grandis wiackowskii Paiva et al ., 2016 based on the morphogenetic stages of left marginal rows. Therefore, we used morphogenesis to classify the subspecies level of the Korean population. This Korean population of U. grandis grandis has intrakinetal anlagen originated from each left marginal row. In contrast, U. grandis wiakowskii has multiple anlagen originated from innermost left marginal cirral row. Fig. 10. Holosticha pullaster in vivo (A-D) and protargol impregnated specimen (E). A. Typical individual and contractile vacuole (arrow). B. Contractile vacuole (arrow). C. Ventral view (arrowheads indicate cytoplasmic granules). D. Dorsal view (arrowheads indicate cytoplasmic granules). E. Oral and somatic ciliature. Scale bars = 20 μm. Fig. 11. Urostyla grandis grandis after protargol impregnation. A. Vegetative stage. B. Middle divider (arrowheads indicate anlagen of left marginal rows). C. Late divider (arrowheads indicate anlagen of left marginal rows). Scale bars= 20 μm. Voucher slides. Two slides of protargol-impregnated specimens were deposited at National Institute of Biological Resources, Korea (NIBRPR0000109470, NIBRPR0000109471).