New data on some Antennoseius Berlese species (Acari: Ascidae) from Russia Author Joharchi, Omid Author Marchenko, Irina I. Institute of Systematics and Ecology of Animals, Novosibirsk, Russia. Author Stanyukovich, Maria K. Zoological Institute of Russian Academy of Sciences, Saint Petersburg, Russia. text Acarologia 2022 2022-03-28 62 2 378 395 https://www1.montpellier.inrae.fr/CBGP/acarologia/article.php?id=4514 journal article 10.24349/4vni-nutt 2107-7207 7160398 Antennoseius ( Vitzthumia ) bregetovae Chelebiev, 1984 Antennoseius ( Vitzthumia ) bregetovae Chelebiev, 1984: 1629 . Antennoseius ( Vitzthumia ) bregetovae Lindquist and Walter, 1989: 1293 ; Beaulieu et al. , 2008: 54; Lindquist and Moraza, 2009: 33 ; Moraza and Kazemi, 2009: 64 ; Moraes et al. , 2016: 67 . ( Figures 1-2 ) Specimens examined — Type material: slide number 1443 with one adult female ( paratype ), Saran, Karaganda province, Kazakhstan , 14.4.1973 , K.A. Chelebiev coll., in nest of Lagurus lagurus (Pallas) ( Rodentia : Cricetidae ); deposited in the ZIN , Saint Petersburg . Other material: two adult females, Aktash, Ulagansky District, Altai Republic , Russia , 50°14′28.0″N 87°41′57.0″E , 30 July 2020 , O. Joharchi coll., on Harpalus sp. ( Coleoptera : Carabidae ). Figure 1 Antennoseius ( Vitzthumia ) bregetovae Chelebiev, 1984 , adult female: A – Idiosoma, dorsal view, enlarged sections not to scale; B – Idiosoma, ventral view; C – Subcapitulum; D – Epistome; E – Chelicera; F – Leg I (trochanter-tibia). Figure 2 DIC micrographs of Antennoseius ( Vitzthumia ) bregetovae Chelebiev, 1984 , adult female: A – Idiosoma, dorsal view; B – Idiosoma, ventral view. Redescription — Female — two specimens measured. Dorsal idiosoma ( Figures 1A & 2A ) — Idiosoma oval (510–515 long × 305–328 wide); dorsal shield divided and lineate-reticulate over entire surface ( Figures 1A & 2A ). Podonotal shield (279–284 long × 262–275 wide) with 19 pairs of setae ( j1–6 , z1–6 , s1–6 , r2 ), r3–6 off shield, of which four pairs j ( 3–5 and z4 ) short (16–18) strongly thickened, spinelike, with a short thin tip ( Figures 1A & 2A ) and nine pairs of pore-like structures, including four pairs of gland openings gd ( 1 , gd2 , gd4 , gd5 ) and five pairs of poroids id ( 1–2, id4–6 ). Setae j1 (11–13), j2 (14–16), z1 (7–9) smooth and short, j1 slightly spinelike, other podonotal shield setae sub-equal (20–28) and mostly smooth, occasionally with 1–2 small barbs in s and r series ( Figures 1A & 2A ). Opisthonotal shield (229–232 long × 262–275 wide), evenly rounded posteriorly, with 15 pairs of setae (19–25) ( J1–5 , Z1–5 , S1–5 ), setae in Z and S series with 1–2 small barbs, Z5 (36–38) longest, and 13 pairs of pore-like structures, including three pairs of gland openings gd ( 6 , gd8 , gd9 ) and ten pairs of poroids idm ( 1–idm6, is1, idl1, idl3, ) idx ( Figures 1A & 2A ); transverse interval between setae J5 about two times shorter than those between J4 and Z5. Lateral soft cuticle with one pair descramble poroid idr ( 6 ) and 21–22 pairs of setae, mostly sparsely barbed, including setae r3-r6 (18–21) and 17–18 pairs of setae R and UR (12–19). Muscle-marks (sigillae) visible mostly on podonotal shield ( Figures 1A & 2A ). Ventral idiosoma ( Figures 1B & 2B ) — Tritosternum with paired sparsely pilose laciniae (90–94), fused basally (13–15), columnar base 20–22 × 13–16 wide; presternal area with pair of narrow indistinct platelets merged to anterior margin of sternal shield, sternal shield length 121–126, width 88–92, fused to endopodal elements between coxae I and II, with a median notch in its anterior margin, posterior margin more or less straight, lineate on lateral margins, with three pairs of smooth subequal setae st1–3 (28–32), and three pairs of poroids ( Figures 1B & 2B ). Metasternal setae st4 (29–31) smooth, inserted on soft cuticle. Endopodal platelets between coxae II-III and III-IV present, small and free. Genital shield drop-shaped length 127–133, width 55–57, faintly lineated laterally, anterior margin of shield convex, not overlapping posterior margin of sternal shield, posterior margin evenly rounded, setae st5 (23–25) on lateral margins of shield, paragenital poroids iv5 located on soft cuticle laterad to shield near setae st5 ( Figures 1B & 2B ). Anal shield obtriangular, length 100–103, width 75–77, with lineate-reticulate over entire surface, paraanal setae (18–20) shorter than post-anal seta (28–30), cribrum goatee-shaped, consisting of a terminal tuft with 3–4 irregular rows of spicules, restricted to area behind postanal seta ( Figures 1B & 2B ); anal gland pores gv ( 3 ) on anterolateral margin of anal shield, posterior to paranal setae, a pair of postanal sigillae present. Soft opisthogastric cuticle with pair of sub-oval metapodal plates (26–28 long × 9–11 wide), nine pairs of smooth setae ( Jv1–5 , Zv1–4 ) (15–26), three pairs of poroids (two ivo ; ivp ) and a gland pores gv2 , laterad to coxae IV. Peritrematal shield fused with exopodal elements along coxae II to IV, anteriorly fused to podonotal shield, with longitudinal lines, evenly rounded posteriorly behind coxae IV, with two pairs of glandular pores and two pairs of poroids; gland pore gp1 located on shield’s ventral edge near abutment of endopodal extension of sternal shield between coxae I and II, poroid ip1 and adjacent gland pore gp2 inserted at level between coxae II and III, and poroid ip2 next to posterior edge of stigma ( Figures 1B & 2B ). Peritremes long, extending from stigmata at mid-level of coxae IV to anterior margin of coxae I ( Figures 1B & 2B ). Gnathosoma ( Figures 1C, 1D & 1E ) — Anterior margin of epistome convex, irregularly denticulate, mid surface of epistome with transverse line of denticles, slightly concave medially ( Figure 1D ). Hypostomal groove with seven transverse rows of denticles, with smooth anterior and posterior transverse lines, all connected laterally, rows 1–5 similar in width, each bearing one or two median denticles, 6 th and 7 th rows slightly widened and with four denticles ( Figure 1C ). Hypostome with four pairs of smooth setae, h1 (31–33)> h3 (25–27)> pc (18–20) > h2 (16–18). Corniculi robust and horn-like, slightly shorter than smooth internal malae. Supralabral process not distinguishable. Labrum blade-like, slightly shorter than internal malae. All palp setae smooth and setiform, palptarsal claw two-tined. Fixed digit of chelicera with an offset distal tooth (gabelzhan), followed by seven small sized teeth, a minute setiform pilus dentilis, and a hyaline flap at basal area, dorsal cheliceral seta thick, prostrate, movable digit with two large teeth medially and a minute tooth subapically, with a ventral projection, arthrodial membrane with a rounded flap, cheliceral lyrifissures distinct ( Figure 1E ). Legs — Legs II (328–332) and III (335–342) short, legs I (430–438) and IV (418–423) longer. Legs I to IV with chaetotactic formulae of femora, genua, tibiae as described for genus. Coxae II–IV lineate on posterior inner surfaces ( Figure 1B ); both setae of coxa I and posterior seta of coxa II modified as spines, pointed abruptly at tip, av on coxa II and setae of coxae III–IV normal, slender ( Figure 1B ). Leg I with setae pd of trochanter and pd2 of femur stout, pointed, spinelike, and with pd 3 of genu stout, spinelike, blunt ( Figure 1F ); pd1 on femur slightly thickened, other setae normal, slender ( Figure 1F ). Legs II–IV without modified setae and smooth. Tibia III having eight setae (2–1/1–2/1–1). Remarks — Antennoseius ( V. ) bregetovae was described from Kazakhstan ( Chelebiev 1984 ). It has been found in nest of steppe vole, Lagurus lagurus (Pallas) ( Rodentia : Cricetidae ). The description of this species is brief and both the description and illustrations lack many important details. Since that time, there has been no other formal occurrence or recording of this species in the world, and is now recorded in Russia for the first time, on ground beetle Harpalus sp. ( Coleoptera : Carabidae ). Lindquist and Moraza (2009) suspected this species to be a senior synonym of Antennoseius ( Vitzthumia ) koroljevae Chelebiev due to both smoothmorph (phoretic) and granular-morph (free-living) occurred in the same locality and habitat, were discussed by Lindquist and Walter (1989) . But according to Chelebiev (1984) and our observations on labels of type series of both species, these two species have been collected from two different nest of Rodents in two different regions and habitats (see provided information in specimens examined section for both species in the current study). In the meantime, A. ( V. ) koroljevae was collected about two years after A. ( V. ) bregetovae . Therefore, we believe these are two distinct species. The diagnosis given above is based primarily on specimens from Russia , but also in comparison with the type material. The species is easily recognized by the podonotal shield with four pairs of spur-like setae j3 ( –5 and z4 ), both setae of coxa I and posterior seta of coxa II modified as spines (pointed abruptly at tip), and leg I with setae pd of trochanter and pd2 of femur stout, pointed, spinelike, and with pd 3 of genu stout, spinelike, blunt.