Earthworms (Oligochaeta, Clitellata) of the Mitaraka range (French Guiana): commented checklist with description of one genus and eighteen species new to science Author Decaëns, Thibaud CEFE, Université de Montpellier, CNRS, EPHE, IRD, 1919 route de Mende, 34293 Montpellier Cedex 5 (France) thibaud. decaens @ cefe. cnrs. fr (corresponding author) thibaud.decaens@cefe.cnrs.fr Author Bartz, Marie Luise Carolina Municipal Center for Culture and Development - Organic Farming: Agriculture and Organic Production Partnership at Centre for Organic and Regenerative Agriculture, Centro Empresarial, Zona Industrial - 6060 - 182, Idanha-a-Nova (Portugal) and Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, 3041 Coimbra (Portugal) bartzmarie @ gmail. com bartzmarie@gmail.com Author Bartz, arolina Author Feijoo-Martínez, Alexander Facultad de Ciencias Ambientales, Universidad Tecnológica de Pereira, A. A. 097, Pereira (Colombia) Author Goulpeau, Arnaud CEFE, Université de Montpellier, CNRS, EPHE, IRD, 1919 route de Mende, 34293 Montpellier Cedex 5 (France) arnaud. goulpeau @ cefe. cnrs. fr goulpeau@cefe.cnrs.fr Author Lapied, Emmanuel Taxonomia Biodiversity Fund, 7 rue Beccaria, 72012 Paris (France) elapied @ yahoo. com elapied@yahoo.com Author Marchán, Daniel F. CEFE, Université de Montpellier, CNRS, EPHE, IRD, 1919 route de Mende, 34293 Montpellier Cedex 5 (France) danifermch @ gmail. com danifermch@gmail.com Author Maggia, Marie-Eugénie Department of Integrative Biology, University of Guelph, N 1 G 2 W 1 Guelph (Canada) mmaggia @ uoguelph. ca mmaggia@uoguelph.ca Author Papuga, Guillaume AMAP, Université de Montpellier, CIRAD, CNRS, INRAE, IRD, 1919 route de Mende, 34293 Montpellier Cedex 5 (France) guillaume. papuga @ umontpellier. fr papuga@umontpellier.fr Author James, Samuel W. Regenerative Agriculture Department, Maharishi International University, Fairfield, IA 52557 (United States) massemaj @ gmail. com massemaj@gmail.com text Zoosystema 2024 2024-04-09 46 9 196 244 https://sciencepress.mnhn.fr/sites/default/files/articles/pdf/zoosystema2024v46a9.pdf journal article 299015 10.5252/zoosystema2024v46a9 5c6e0140-35bc-4e1e-9c60-4a7c2e340019 1638-9387 12551981 9AB092A6-A017-42EF-BEF3-44F533D6C88C Martiodrilus ( Botaria ) lavellei Decaëns & Bartz , n. sp. ( Fig. 7 ) urn:lsid:zoobank.org:act: AFE58CB8-CEE3-46FE-8576-0259AAF5E274 TYPE MATERIAL . — Holotype . French Guiana • Adult ; Tumuc-Humac , Mitaraka Massif , tropical rainforest on DIADEMA project A trail, in the soil; 2°14’27”N , 54°27’15”W ; 359 m a.s.l. ; III.2015 ; T. Decaëns , E. Lapied leg.; BOLD Sample ID: EW-MI15-0105 ; MNHN . ETYMOLOGY . — This species is named in honour of Patrick Lavelle, in acknowledgement to his contribution for the study of tropical earthworm ecology. ECOLOGY . — This species is only known from the holotype specimen, which has been collected in the organo-mineral soil of a plateau forests ( Fig. 7D, E ). DISTRIBUTION . — Martiodrilus ( B. ) lavellei Decaëns & Bartz , n. sp. is only known from the Mitaraka Massif. DESCRIPTION External morphology ( Fig. 7A, B ) Body shape cylindrical. Body pigmentation dorsally light brown, ventrally beige. Body length: 56 mm after ethanol fixation. Body mass: 0.19 g after ethanol fixation. Diameter: 3.4 mm in the preclitellar region, 4.2 mm in the clitellum, 2.5 mm in the postclitellar region. Number of segments: 131. Prostomium proepilobic. One pair of longitudinal grooves going anterior to posterior through segment I in line of nephridial pores. Setae closely paired, ab beginning in II, cd in III. Setal arrangement aa:ab:bc:cd:dd = 8:1:10:2:34. Clitellum in (dorsal XV) XVI-XXV, saddle-shaped ( Fig. 7A ). Genital markings in V-XXIII, intraclitellar in XV-XXV ( ab position). Tubercula pubertatis linear in 1/4 XX-½ XXV ( Fig. 7B ). Male pores not recognised, and ovipores not visible. Spermathecal pores in 5/6, 6/7 and 7/8, near nephridial pore. Nephridial pores begining in III, in CD line. FIG . 6. — Martiodrilus ( Botaria ) flavus Decaëns & Bartz n. sp. : A , external and lateral view of the anterior body ( arrows indicate the clitellum position); B , external and semi-ventral view of the anterior body ( arrows indicate the position of the tubercula pubertatis); C , dorsal dissection showing anterior internal anatomy; D , detail on the spermathecae; E , detail on the typhlosole when rolled up; F , detail on the typhlosole when unrolled; G , habitat preferences; H , microhabitat preferences.All images from the holotype specimen: CL , clitellum; DV , dorsal vessel; GZ , gizzard; IN , intestine; SP , spermathecae; SV , seminal vesicles; TF , typhlosole; TP , tubercula pubertatis; N , total number of specimens in the dataset. Scale bars: A-C, E, F, 10 mm ; D, 5 mm . FIG . 7. — Martiodrilus ( Botaria ) lavellei Decaëns & Bartz n. sp. : A , external and lateral view of the anterior body ( arrows indicate the clitellum position); B , external and ventral view of the anterior body ( arrows indicate the position of the tubercula pubertatis); C , dorsal dissection showing anterior internal anatomy; D , habitat preferences; E , microhabitat preferences.All images from the holotype ; CL , clitellum; DV , dorsal vessel; GZ , gizzard; IN , intestine; SP , spermathecae; SV , seminal vesicles; TP , tubercula pubertatis; N , total number of specimens in the dataset. Scale bars: 2.5 mm . Internal anatomy ( Fig. 7C ) Septa : membranous. Gizzard: muscular and well developed in VI, 1.80 × 2.30 mm (width × length). Calciferous glands: eight pairs, in VII-XIV with tubular composite structure. Esophagus-intestine transition in XVIII/XIX; intestine without caeca.Typhlosole abruptly begining in XXVI, structured as a long thin folded lamella occupying all the lumen. Hearts: five pairs, the two intestinal pairs well-developed in X-XI. Excretory apparatus holoic, nephridia with simple nephrostome. Testes sacs: semi-periesophageal in X and XI, enclosing basal part of hearts, calciferous glands and seminal vesicles. Seminal vesicles: two pairs in XI-XII, small lobulated occupying their segments only. Spermathecae: three pairs, VI, VII, VIII; ovoid elongated without diverticula. REMARKS Martiodrilus ( B. ) lavellei Decaëns & Bartz , n. sp. belongs to the subgenus Botaria because of the presence of three pairs of spermathecae. It differs from the majority of species in the subgenus by its clitellum beginning in XVI, a position which it shares only with the two Guyanese species Martiodrilus ( Botaria ) dewynteri Csuzdi & Pavlicek, 2011 and Martiodrilus ( Botaria ) helleri ( Michaelsen, 1918 ) . Although this needs to be confirmed on a larger number of specimens, the clitellum of M. ( B. ) lavellei Decaëns & Bartz , n. sp. seems less extended posteriously than in these two species, ending in XXV in the holotype specimen instead of XXVI to ½ XXVI. The position of tubercula pubertatis also differs among these species: 1/4 XX-½ XXV in M. ( B. ) lavellei Decaëns & Bartz , n. sp. instead of ½ XXI-½XXVI in M. ( B. ) dewynteri and XX-½ XXVI in M. ( B. ) helleri . Furthermore, M. ( B. ) lavellei Decaëns & Bartz , n. sp. is significantly smaller than M. ( B. ) helleri , and appears to have a lighter body pigmentation than M. ( B. ) dewynteri (light brown instead of brown-violet). Martiodrilus ( B. ) lavellei Decaëns & Bartz , n. sp. is clearly separated from other species of Martiodrilus from the Mitaraka Massif by its COI barcode ( Table 2 ). This species corresponds to OTU#080 in Maggia et al. (2021) .