Additions and corrections to taxonomy of Apobaetis fiuzai Salles & Lugo-Ortiz, 2002 and Apobaetis kallawaya Nieto, 2006 (Ephemeroptera, Baetidae) Author De Lima, Cláudia R. T. 18795D2E-427D-4A26-B112-70C929CA5B95 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Laboratório de Citotaxonomia e Insetos Aquáticos, CEP 69067 - 375, Manaus, Amazonas, Brazil. Universidade Federal de Rondônia (UNIR), Departamento de Biologia, Laboratório de Biodiversidade e Conservação, 76.801 - 059, Porto Velho, Rondônia, Brazil. claudia.tavares.lab@gmail.com Author Cruz, Paulo Vilela 34F85F41-2743-4248-813F-0CEEE63FE649 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Laboratório de Citotaxonomia e Insetos Aquáticos, CEP 69067 - 375, Manaus, Amazonas, Brazil. Universidade Federal de Rondônia (UNIR), Departamento de Biologia, Laboratório de Biodiversidade e Conservação, 76.801 - 059, Porto Velho, Rondônia, Brazil. pvilelacruz@gmail.com Author Hamada, Neusa 0D02F36C-6B09-423C-95A6-6179E9808CD5 Instituto Nacional de Pesquisas da Amazônia (INPA), Coordenação de Pós-Graduação (COPOG), Divisão do Curso em Entomologia (DiEnt), Coordenação de Biodiversidade (CoBio), Laboratório de Citotaxonomia e Insetos Aquáticos, CEP 69067 - 375, Manaus, Amazonas, Brazil. Universidade Federal de Rondônia (UNIR), Departamento de Biologia, Laboratório de Biodiversidade e Conservação, 76.801 - 059, Porto Velho, Rondônia, Brazil. neusaha@gmail.com text European Journal of Taxonomy 2023 2023-07-13 879 1 136 161 http://dx.doi.org/10.5852/ejt.2023.879.2167 journal article 55107 10.5852/ejt.2023.879.2167 ce30301f-248e-4674-bd0c-fcfad4f875ee 2118-9773 8155397 A49B65B5-F27A-44EC-9A13-A39BF72F6E61 Apobaetis pasternakae sp. nov. urn:lsid:zoobank.org:act: CA9DDFA5-45BE-44FC-9EC5-A92B09F133B8 Figs 1 , 3–5 Apobaetis fiuzai – Cruz et al. 2011: 89 . –– Falcão et al. 2011: 519 . –– Boldrini et al. 2012: 92 . Diagnosis MALE IMAGO (adapted from Cruz et al. 2011 ). Wing ( Cruz et al. 2011 : fig. 8). hyaline, veins light brown; stigmatic area with eight veins not touching Sc vein; marginal intercalary veins paired, except single between veins IMP2 and CuA, absent between CuA and A; length of each intercalary vein 0.6× distance between adjacent longitudinal vein; length of forewing about 2.5 × width. External genitalia ( Cruz et al. 2011 : fig. 10a–b). Area between unistyligers with a deep V emargination. Internal genitalia ( Fig. 5H ). A pair of sclerotized gonovectes V-shaped, broad apex (gv); retractor muscle of the gonovectes fixed in the angle region of the gonovectes (m.gv); sclerotized penial bridge (pb); a pair of styligeral muscle (ms). NYMPH . Characterized by a combination of the following characters: 1) labrum rectangular, distal medial margin with one protuberance; dorsal surface with 3 to 5 elongated and blunt medial setae near distal margin ( Fig. 4A–B ); 2) hypopharynx with lingua subcircular, with apical tuft of setae, length subequal to superlingua ( Fig. 4E ); 3) maxillary palp long, greater than or equal to 2.0× the length of galea-lacinia; segment II tapering abruptly from the apical half to the apex, without apical constriction ( Fig. 4F ); 4) labial palp segment II with triangular pointed distomedial projection, apically directed; segment III rectangular, distal margin slightly concave ( Fig. 4G ); 5) foreleg with dorsal margin of femur with one row of 11 to 13 concave and blunt long setae; claw 0.6–0.8× length of tarsus, without row of denticles ( Fig. 5A ); 6) posterior margin of tergum IV with triangular and pointed spines in irregular sizes ( Fig. 5C ); 7) paraproct with several marginal spines, posterolateral extension without spines ( Fig. 5E ). Etymology The species name is a tribute to Dr Natália Pasternak, founder of the Instituto Questão de Ciências in São Paulo . During the Coronavirus Pandemic, she acted fighting fake news. Material examined Holotype BRAZIL • nymph on slide; Amazonas State , Manaus , Reserva Florestal Adolpho Ducke , stream Barro Branco ; 02º53′ S , 59º58′ W ; 10 Mar. 2009 ; R . Boldrini and P. V . Cruz leg.; INPA . Paratypes BRAZIL – Amazonas • 2 nymphs on slide; same collection data as for holotype; INPA • 5 nymphs in alcohol 80%; same collection data as for holotype; INPA . Additional material BRAZIL – Amazonas • 12 ♂♂ imagoes in 80% alcohol; Manaus , Reserva Florestal Adolpho Ducke , stream Barro Branco ; 02º53′ S , 59º58′ W ; 8Apr. 2009 ; R . Boldrini leg.; INPA • 5 males imagoes genitalia on slide; same collection data as for preceding; INPA • 6 nymphs on slide; Presidente Figueiredo , Corredeira da Pantera ; 02º02′ S , 59º50′ W ; 8 Nov. 2009 ; R . Boldrini and P. V . Cruz leg.; INPA • 1 nymph on slide; same collection data as for preceding; 12 Nov. 2009 ; INPA • 3 nymphs on slide; Presidente Figueiredo , Cachoeira do Santuário ; 02º03′ S , 59º55′ W ; 13 Nov. 2009 ; R . Boldrini and P. V . Cruz leg.; INPA • 1 nymph on slide; Apuí ; 07º11′ S , 59º53′ W ; 2 Jul. 2018 ; P. V . Cruz, G . Desidério and N. Hamada leg.; INPA . – Roraima • 2 nymphs on slide; Caroebe , Rio Caroebe , ramal 37, Cachoeirinha farms; 00º57′09.2″ N , 59º37′00.5″ W ; 23 Mar. 2012 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA • 12 nymphs in alcohol 80%; same collection data as for preceding; INPA • 1 nymph in alcohol 80%; Caroebe , Lago Jacundá , Vicinal 02; 00º50′59.8″ N , 59º40′48.2″ W ; 29 Nov. 2006 ; J.N. Falcão leg.; INPA • 2 nymphs on slide; São João da Baliza , sítio do igarapé; 01º00′59.7″ N , 59º55′53.1″ W ; 24 Mar. 2012 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA • 3 nymphs in alcohol 80%; same collection data as for preceding; INPA • 3 nymphs on slide; Caroebe , Igarapé do Jacaré , vicinal 05; 01º03′58.2″ N , 59º3′06.8″ W ; 19 Mar. 2013 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA • 4 nymphs on slide; Caroebe , Rio Caroebe , vicinal 05; 01º03′58.2″ N , 59º3′06.8″ W ; 12 Mar. 2018 ; P. V . Cruz and I.O. Fernandes leg.; INPA • 1 nymph on slide; Pacaraima , Rio Ereu ; 01º56′01.3″ N , 61º01′38.4″ W ; 26 Mar. 2012 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA • 1 nymph on slide; Boa Vista , Rio Murupu ; 03º01′16.3″ N , 60º46′32.9″ W ; 19 Dec. 2004 ; N. Hamada and F.F. Salles leg.; INPA • 1 nymph on slide; Bonfim , Rio Arraia ; 03º21′01.6″ N , 59º54′14.5″ W ; 5 Nov. 2006 ; J.N. Falcão leg.; INPA • 1 nymph in alcohol 80%; Caroebe , Lago Jacundá , first bridge, Vicinal 2; 00º50′59.8″ N , 59º40′48.2″ W ; 29 Nov. 2008 ; J.N. Falcão leg.; INPA • 2 nymphs on slide; Bonfim , Rio Arraia ; 03º21′04.0″ N , 59º54′13.5″ W ; 27 Mar. 2012 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA • 2 nymphs in alcohol 80%; same collection data as for preceding; INPA • 2 nymphs on slide; Cantá , Rio do Cachorro bridge; 02º25′20.2″ N , 60º40′00.9″ W ; 28 Mar. 2012 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA • 2 nymphs on slide; BR 170 ; 02º08′59.9″ N , 60º40′39.9″ W ; 28 Mar. 2012 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA . – Rondônia • 3 nymphs on slide; Candeias do Jamarí , Igarapé da Onça ; 08º52′40.0″ S , 63º38′02.2″ W ; 9 Jul. 2016 ; N. Hamada and P. V . Cruz leg.; INPA . – Maranhão • 2 nymphs on slide; Estreito , BR 010 , near the bridge, Rio Farinha ; 06º31′47.3″ S , 47º28′11.4″ W ; 22 Jul. 2010 ; N. Hamada , P. V . Cruz, G . Dantas and R . Boldrini leg.; INPA . Description Nymph LENGTH . Body: 2.9–3.1 mm . HEAD . Antenna. Flagellum with minute spines at apex of each flagellomere. Labrum ( Fig. 4A–B ). Length about 0.5 × maximum width; distal medial margin with one protuberance; dorsal surface with 3 to 5 elongated and blunt medial setae near distal margin; medially with one row of long and thin setae near distal margin; long, thin and simple setae covering dorsal surface; ventral surface with spine-like setae on distolateral and distal margins. Left mandible ( Fig. 4C ). Incisors not fused; outer and inner set of incisors with 4 and 3 denticles, respectively; prostheca robust, bifurcated at apex, outer lobe robust, inner lobe slender with medial tuft of setae; margin between prostheca and mola concave; subtriangular process wide with short protuberance on distal margin; tuft of setae at base of subtriangular process; denticles of mola not constricted; lateral margin convex. Right mandible ( Fig. 4D ). Incisors not fused; outer and inner set of incisors each with 3 denticles; prostheca slender, bifurcated at apex; margin between prostheca and mola concave; tuft of setae at base of mola absent; denticles of mola not constricted; lateral margin convex. Hypopharynx ( Fig. 4E ). Lingua subcircular, with apical tuft of setae, length subequal to superlingua; superlingua not expanded, with short, fine and simple setae scattered over distolateral and distal margin. Maxilla ( Fig. 4F ). Maxillary palp long, greater than or equal to 2.0 × length of galea-lacinia; segment I 0.7× length of galea-lacinia; segment II tapering abruptly from apical half to apex, with fine and simple setae scattered over surface, without apical constriction. Labium ( Fig. 4G ). Glossa subquadrangular, medially broad, subequal to paraglossa; dorsal surface with one longitudinal row of 4 short spine-like setae near inner margin; apex with 3 short spine-like setae; longitudinal row of 9 robust spine-like setae near outer margin; ventral surface covered with long, thin and simple setae. Paraglossa curved inward; dorsal surface with long and robust setae on apex; outer margin with one longitudinal row of 16 long spine-like setae to base and one longitudinal row of 6 long and robust spine-like setae to base near inner margin; ventral surface with one longitudinal row of 7 long and robust spine-like setae at middle, at apical half. Labial palp with segment I 0.8 × length of segments II and III combined, covered with micropores (not illustrated); segment II with outer margin covered with thin, long and simple setae, inner margin bare; segment II with triangular pointed distomedial projection apically directed; ventral surface of distomedial projection with tuft thin, long and simple setae; segment III rectangular, distal margin slightly concave, length 0.6× width, covered with fine, long and simple setae on outer margin, dorsally with a row of 11 to 14 robust setae in different sizes near distal margin, ventrally with 10 to 12 robust spine-like setae in different sizes near distal margin. THORAX . Holotype pigment ( Fig. 3A–C ). Light yellow; femur with rounded brown mark on apical third. Foreleg ( Fig. 5A–B ). Femur: dorsal margin with one row of 11 to 13 concave and blunt long setae; apex with 2 concave and blunt long setae; ventral margin with one row of 6 to 8 elongated spine-like setae; posterior surface with one row of 8 to 10 elongated spine-like setae near ventral margin. Tibia: dorsal margin bare, ventral margin with one row of 8 to 10 spine-like setae. Patella-tibial suture present, from dorsal to ventral margin. Tarsus: dorsal margin bare, ventral margin with one row of 12 to 15 spine-like setae. Claw: 0.6–0.8× length of tarsus, row of denticules absent. Middle and hindleg similar to foreleg. ABDOMEN . Holotype pigment ( Fig. 3A–C ). Light yellow; terga I and II with medial brown mark; tergum V with lateral brown mark; tergum IX with brown mark on anterior margin; sterna VI–VIII with anterolateral light brown mark almost imperceptible; sternum IX with brown mark on lateral and anterior margin. Tergum IV ( Fig. 5C ). Terga surface covered by triangular scale-like projections and by micropores; posterior margin of tergum IV with triangular and pointed spines, in irregular sizes. Gill ( Fig. 5D ) apex rounded, trachea conspicuous; median length, extending to half of second subsequent tergum. Paraproct ( Fig. 5E ) with several marginal spines; posterolateral extension without spines. Cerci ( Fig. 5F ) short lateral spines on all segments. Paracercus ( Fig. 5G ) short lateral spines on all segments. Male imago INTERNAL GENITALIA ( Fig. 5H ). A pair of unistyligers cylindrical, completely separated one from another (us); sclerotized penial bridge articulated with the gonovectes and the X tergum abdominal (pb); pair of sclerotized gonovectes V-shaped, broad apex (gv) with an attached membrane on lateral parts (mb); a pair of gonovectal muscle goes from gonovectes apex to lateral parts of IX sternum (m.gv); a pair of median styligeral muscle goes from lateral parts of membrane to posterior margin of IX sternum (ms). Intraspecific variation of Apobaetis pasternakae sp. nov. Pigment variation THORAX . Light yellow, with short brown spots ( Fig. 3D, F, 3J, M ); brown mark covering almost completely the thorax ( Fig. 3H ); femur without marks ( Fig. 3G ); forewing pads with brown mark on basal third ( Fig. 3H ). ABDOMEN . Terga I–III with brown spots ( Fig. 3D, F ); terga I–X with short light brown marks ( Fig. 3J ); tergum I with medial brown mark, terga II and III with brown marks, terga IV–X with light brown spots ( Fig. 3M ); sterna VII and VIII with anterolateral brown mark ( Fig. 3G ); sterna I–VIII with anterolateral brown mark, sterna I–VIII with lateral brown mark ( Fig. 3L, N ); cerci e paracercus with light brown trio marks, alternating over of length ( Fig. 3D, J, M ). Morphological variation LENGTH . Body 2.2–3.1 mm .