Additions to Italian Pleosporinae, including Italica heraclei sp. nov. Author Wijesinghe, Subodini N. https://orcid.org/0000-0002-5625-9578 Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Wang, Yong Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China yongwangbis@aliyun.com Author Zucconi, Laura Department of Ecological and Biological Sciences, University of Tuscia, Largo dell'Universita snc, 01100, Viterbo, Italy Author Dayarathne, Monika C. Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China Author Boonmee, Saranyaphat Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand Author Camporesi, Erio A. M. B. Gruppo Micologico Forlivese " Antonio Cicognani ", Via Roma 18, Forli, Italy Author Wanasinghe, Dhanushka N. https://orcid.org/0000-0003-1759-3933 CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China & East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming 650201, Yunnan, China & Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County, Yunnan, China Author Hyde, Kevin D. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand & CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China & Innovative Institute of Plant Health, Zhongkai University of Agriculture and Enginnering, Haizhu District, Guangzhou 510225, China text Biodiversity Data Journal 2021 2021-01-18 9 59648 59648 http://dx.doi.org/10.3897/BDJ.9.e59648 journal article http://dx.doi.org/10.3897/BDJ.9.e59648 1314-2828-9-e59648 2E56CD86B4425E9A8825FFF492BF19C8 Phomatodes nebulosa (Pers.) Qian Chen & L. Cai, Stud. Mycol. 82: 191 (2015) Phomatodes nebulosa Sphaeria nebulosa Sphaeria nebulosa = Sphaeria nebulosa Pers., Observ. mycol. (Lipsiae) 2: 69 (1800) [1799] Materials Type status: Other material . Occurrence: recordedBy: Erio Camporesi ; Taxon: namePublishedIn: Phomatodes nebulosa (Pers.) Qian Chen & L. Cai, Stud. Mycol. 82: 191 (2015); kingdom: Fungi; phylum: Ascomycota; class: Dothideomycetes; order: Pleosporales; family: Didymellaceae; genus: Phomatodes; specificEpithet: nebulosa; taxonRank: species; Location: stateProvince: Province of Arezzo [AR]; county: Italy; municipality: near Passo la Calla - Stia; Identification: identifiedBy: S.N. Wijesinghe ; Event: year: 2018; month: December; day: 3; habitat: on a dead and aerial stem of Urtica dioica (Rosales, Urticaceae); fieldNotes: Terrestrial; Record Level: institutionID: MFLU 18-2685; institutionCode: Mae Fah Luang University Herbarium (MFLU) ; ownerInstitutionCode: IT 4110 Type status: Other material . Record Level: type: living culture; collectionID: MFLUCC 20-0155; collectionCode: Mae Fah Luang Culture Collection (MFLUCC) Description Saprobic on dead aboveground stem of Urtica dioica L. ( Rosales , Urticaceae ). Asexual morph : Coelomycetous. Conidiomata (Fig. 5 a-c) immersed, raised as black spots on the host surface, pycnidial, 60-70 x 140-170 µm (x¯ = 66.5 x 155 µm , n = 10), solitary, scattered, unilocular, globose or subglobose to irregular. Pycnidial wall (Fig. 5 d) pseudoparenchymatous, 3-5-layered, 15-30 µm (x¯ = 25 µm , n = 10) wide, thick walled, the outermost layer comprising dark brown cells of textura angularis , the inner layer comprising pale brown to hyaline cells of textura angularis . Conidiophores reduced to conidiogenous cells. Conidiogenous cells (Fig. 5 e-f) 4-5 x 2-4 µm (x¯ = 4.5 x 3.6 µm , n = 5), enteroblastic, phialidic, ampulliform or short cylindrical, determinate, smooth, hyaline. Conidia (Fig. 5 g-j) 4-7 x 1-2 µm (x¯ = 5.3 x 1.6 µm , n = 30) ellipsoidal to cylindrical, aseptate, guttulate, smooth-walled, hyaline. Sexual morph : Undetermined. Culture characteristics: Conidia germinating on PDA within 24 h, from single-spore isolation. Colonies (Fig. 5 l-m) on PDA reaching 5-10 mm diam. after 10 days at 18°C, circular, entire edge, flat, dense, white in both upper and lower sides. GenBank accession numbers (ex-MFLUCC 20-0155): ITS = MT880293, LSU = MT880295, TUB2 = MT901291 Notes Phomatodes was introduced by Chen et al. (2015) to accommodate Phoma -like taxa in Didymellaceae . The type species, Phomatodes aubrietiae , is characterised by globose to subglobose pycnidia, ostiolate conidiomata, solitary or confluent, with a 3-5-layered, pigmented pseudoparenchymatous pycnidial wall, phialidic, hyaline, smooth, ampulliform to doliiform conidiogenous cells and cylindrical to allantoid, hyaline, thin-walled, smooth, aseptate, polar guttulate conidia ( Chen et al. 2015 ). The morphology of our material (Fig. 5 ) agrees with that of the holotype (CBS 100191), with globose to subglobose conidiomata; phialidic, ampulliform conidiogenous cells; and hyaline, aseptate and polar guttulate conidia (5-7 x 1.5-2.5 µm ). From the comparison of ITS, LSU and TUB2 sequences between P. nebulosa (CBS 100191-type) and P. nebulosa (MFLUCC 20-0155), both strains were identical. In our multi-locus phylogenetic analyses, the new isolate (MFLUCC 20-0155) and the ex-type strains of P. nebulosa (CBS 117.93, CBS 740.96, CBS 100191, MFLU 18-0177) clustered together with high support (99 ML/1.00 PP) (Fig. 2 ). Early records of Phomatodes nebulosa were reported on Armoracia rusticana ( Brassicales , Brassicaceae ) and Mercurialis perennis ( Malpighiales , Euphorbiaceae ) from the Netherlands, Thlaspi arvense ( Brassicales , Brassicaceae ) from Poland ( Chen et al. 2015 , Farr and Rossman 2020 ) and Datisca cannabina ( Cucurbitales , Datiscaceae ) from Uzbekistan ( Gafforov 2017 , Farr and Rossman 2020 ). Our new strain of P. nebulosa from U. dioica was collected from the Province of Arezzo in Italy at higher altitude (296 m a.s.l.), compared to the previous Italian record on the same host, but from the Province of Forli-Cesena (34 m a.s.l.) ( Hyde et al. 2020a ). Considering the results of our integrative taxonomic approach, we report this strain as a new record of P. nebulosa , the first for the Province of Arezzo and the second for Italy, widening its geographic distribution in the country.