Five new species of Trichoderma from moist soils in China Author Zhang, Guang-Zhi Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China Author Yang, He-Tong Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China Author Zhang, Xin-Jian Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China zhangxj@sdas.org Author Zhou, Fang-Yuan Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China Author Wu, Xiao-Qing Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China Author Xie, Xue-Ying Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China Author Zhao, Xiao-Yan Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China Author Zhou, Hong-Zi Qilu University of Technology (Shandong Academy of Sciences), Ecology Institute, Shandong Provincial Key Laboratory for Applied Microbiology, Jinan 250103, China text MycoKeys 2022 2022-02-17 87 133 157 http://dx.doi.org/10.3897/mycokeys.87.76085 journal article http://dx.doi.org/10.3897/mycokeys.87.76085 1314-4049-87-133 3E84DC4D23DD55D1BD51C48DF991EADD Trichoderma hailarense G.Z. Zhang sp. nov. Fig. 3 Etymology. The specific epithet " Trichoderma hailarense " refers to the locality, the Hailar River Basin in Inner Mongolia of China where the holotype was found. Typification. China. Inner Mongolia, Hailar River Basin, 618 m (altitude), isolated from soil, 17 September 2016, G.Z. Zhang (Holotype WT 17901). Diagnosis. Phylogenetically, Trichoderma hailarense formed a distinct clade and is related to T. gamsii and T. neokoningii (Fig. 1 ). The sequence similarity of rpb2 with T. gamsii S488 and T. neokoningii CBS120070 was 97.32% and 96.86%, respectively and the sequence similarity of tef1 -α with T. gamsii S488 and T. neokoningii CBS120070 was 97.43% and 96.66%, respectively. Colonies of T. hailarense did not form conidia on PDA and conidia of T. hailarense on other media were obovoid, delicately roughened and easily distinguished from those of T. gamsii and T. neokoningii . Teleomorph. Unknown. Growth optimal at 30 °C, slow at 35 °C on all media. Colony radius after 72 h at 30 °C 53-56 mm on PDA, 54-56 mm on CMD, 33-37 mm on MEA and 33-36 mm on SNA. Colony radius after 72 h at 35 °C 13-15 mm on PDA, 10-14 mm on CMD, 9-12 mm on MEA and 10-12 mm on SNA. Aerial mycelia abundant, arachnoid on PDA after 72 h at 25 °C under 12 h photoperiod. Conidiation started around the inoculation point after 7 days on PDA, with relatively few or small conidia. Diffusing pigment or distinctive odour absent. Conidiation started around the inoculation point after 7 days on MEA, forming a few large pustules, cream yellow. On SNA, aerial mycelia were few, forming a few large pustules around the inoculation point in age, cream-yellow. Conidiophores and branches narrow and flexuous, tending to be regularly verticillate, forming a pyramidal structure, with each branch terminating in a cruciate whorl of up to five phialides. Phialides, lageniform, (8.0-)9.4-13.1(-15.5) x (2.5-)3.0-3.5(-3.6) μm (mean = 11.2 x 3.3 μm ), base 1.8-2.5 μm (mean = 2.1 μm ); phialide length/width ratio (2.33-)2.7-4.4(-5.9) (mean = 3.4). Conidia obovoid, (4.2-)4.3-4.7(-4.9) x (3.4-)3.6-3.9(-4.1) μm (mean = 4.5 x 3.7 μm ), length/width ratio 1.1-1.4 (mean = 1.2), delicately roughened. Chlamydospores: (7.0-)7.5-8.2(-8.5) x (6.5-)7.0-7.5(-8.3) μm . Figure 3. Trichoderma hailarense A-D cultures on different media incubated at 25 °C for 14 days ( A on PDA B on MEA C on CMD D on SNA) E, G-K conidiophores and phialides F chlamydospores L, M conidia. Notes: E on MEA F-M on PDA A-M from WT17901. Scale bars: 10 μm ( E-J ). Distribution. China. Inner Mongolia. Additional specimen examined. China. Inner Mongolia, Hulun Buir, 610 m (altitude), isolated from soil, 17 September 2016, J.D. Hu (WT17905). Notes. Phylogenetically Trichoderma hailarense is related to T. gamsii and T. neokoningii (Fig. 1 ) and does not meet the sp ∃!( rpb2 99≅ tef1 97) standard for T. gamsii or T. neokoningii . Morphologically, colonies of T. gamsii and T. neokoningii on PDA formed conidia sporadically or in hemispherical pustules and conidia of T. gamsii and T. neokoningii were ellipsoidal to oblong, smooth-walled ( Jaklitsch et al. 2006 ). However, colonies of T. hailarense did not form conidia on PDA and conidia of T. hailarense on other media were obovoid, delicately roughened and easily distinguished from those of T. gamsii and T. neokoningii .