New stiletto flies from New Caledonia (Therevidae, Agapophytinae) Author Irwin, Michael E. Emeritus, University of Illinois, Urbana-Champaign, Illinois, USA & University of Arizona, Tucson, USA Author Winterton, Shaun L. California State Collection of Arthropods, California Department of Food & Agriculture, Sacramento, California, USA wintertonshaun@gmail.com Author Metz, Mark A. USDA, ARS, Systematic Entomology Laboratory, Beltsville, Maryland, USA https://orcid.org/0000-0002-3535-535X text ZooKeys 2020 984 83 132 http://dx.doi.org/10.3897/zookeys.984.53587 journal article http://dx.doi.org/10.3897/zookeys.984.53587 1313-2970-984-83 D8B945AE620949578BB1D96DDBB5AA44 1448149CA44B51ED9EE57DC3A924C430 Jeanchazeauia nubilosus sp. nov. Figs 3 , 6E , 7H , 23C , 25 , 26 , 27A-F , 30 , 31A, C , 32B Diagnosis. Black macrosetae on legs; frons dark, sparsely pubescent; female abdomen dark with white bands; female wing with strong banding, irregular basally, wing apex dark; male wing with uniform infuscation. Description. Length 8.3-9.6 mm. Head . Black to brown; mostly silver pubescent, upper frons sparsely pubescent, admixed with black setae, longer in male, a few setae dorsolateral to antennal bases, eyes separated by width of ocellar tubercle in both sexes; parafacial black with silver pubescence. Occiput black, matte pubescent, more bronze coloured along postocular ridge, silver laterally and ventrally. Postocular macrosetae mostly black, in a single row in both sexes, multiple irregular rows laterally, replaced by white finer setae laterally and on gena. Scape 1/2x head length; dark brown, sparsely silver pubescent; long, black, fine filiform setose except medial surface bare of setae. Basal flagellomere 1/2x length of scape, elongate and gradually tapering to a blunt point apically; sparsely silver pubescent with short, fine, black setae around base. Second flagellomere slightly conical, apex narrower than base; <1/10 x length of basal flagellomere. Third flagellomere subequal in length to second, conical. Style small, spiculate. Palpus one segmented, cylindrical, dark brown basally, light brown apically; silver pubescent admixed with white, setae basally, brown apically. Mouthparts brown with brown setae. Thorax . Dark brown-black with black and silver pubescence; scutum brown pubescent with anterior postpronotal lobe and notopleuron sparsely silver pubescent; scutellum with black matte pubescence anteriorly and medially, bronze pubescent along posterior margin; pleuron silver pubescent with two brown glabrous vertical bands passing from the notopleuron at the prothoracic spiracle posteroventrally through the anterior katepisternum, and from the wing base posteroventrally through the meron; macrosetae black (np: 3, sa: 1, pa: 1, dc: 1, sc: 1); scutum with short, black setae; postpronotal lobe, cervical sclerite, proepisternum, and lateral prosternum with white setae; katatergite with mostly white setae, both sides with one dark brown seta. Legs . Dark brown; coxae with dense silver pubescence, otherwise sparsely pubescent and admixed with short, black setae; macrosetae mostly black with a few light brown macrosetae on hind tibia. Coxae with white setae; forecoxa with two, midcoxa with three, and hind coxa with five black, anteroventral, marginal macrosetae; hind coxa lacking lateral seta. Posterior surface of femora with long, white setae admixed with short, black setae. Hind femur with one or two subapical anteroventral macrosetae; series of minute posteroventral macrosetae barely evident along middle of femur. Wing . Male wing membrane with entirely grey infuscation, darker subapically near anterior margin and apices of cells br and bm; membrane completely covered with microtrichia. Female wing strongly banded, with extensive hyaline areas; pterostigma brown; venation brown, cell m3 widely open at wing margin. Haltere entirely dark brown, knob slightly lighter ventrally. Abdomen . Dark brown-black, short, black, setae on all segments, tergites I and II laterally and sternites I-V with long, white setae; male tergites II-IV and intersegmental membrane on sternites II and III covered with silver velutum pubescence. Genitalia . Male: tergite VIII emarginate medially; black setose laterally on posterior 1/2. Sternite VIII quadrate, slightly wider posteriorly, posterior margin black setose. Epandrium quadrate, emarginate anteriorly and posteriorly, lateral corners only slightly extended posteriorly; dark brown setae longer posterolaterally. Cerci bluntly pointed posteriorly, dark brown setae dorsally and apically. Subepandrial sclerite wide, 1/2 width of epandrium; partially sclerotised, lateral margins more strongly so. Gonocoxites rounded with subtriangular outer gonocoxal process; long, dark setae, densely spaced laterally and small patch ventromedially; inner gonocoxal process smoothly curving medially, acuminate, lacking setae apically; ventral lobe minutely setose ventrally, gonostylus curved dorsomedially with a broad, dorsal process at 2/3 of length with medial and lateral carinae basodorsally, dark brown setose ventrally and on medial face. Aedeagus with dorsal apodeme of parameral sheath 'T' -shaped, narrow posteriorly and widened anteriorly with anterolateral corners curving ventrally and laterally; distiphallus wide basally with lateral carinae, narrow apically, distiphallus spinose laterally; ventral apodeme dorsoventrally flattened, bifurcations subparallel to ejaculatory apodeme; ejaculatory apodeme robust, roughly cylindrical; posterior end of ejaculatory apodeme broadened laterally and ventrally expanded to a point creating a basket-shaped posterior face, lateral ejaculatory apodeme short and subtriangular with lateral process; basiphallus membranous anteriorly. Female: tergites VII and VIII highly modified, much wider than long, with a long and narrow anteromedial process; posterior margin slightly emarginate; with elongate dark setae. Sternite VIII slightly longer than wide, convex ventrally, posterior lobes tapering sharply posteriorly, separate, with a median aedeagal guide; extensive elongate, orange setae, bare at extreme lateral margin. Median lobe of tergite IX reduced, very short and not sclerotised. Acanthophorite with acanthophorite spines dark brown, A2 series spines indistinguishable from rest of acanthophorite setae. Sternite X quadrate. Cercus slightly laterally flattened, extended posteriorly, longer than wide, membranous, slightly sclerotised; furca longer than wide, semicircular anteriorly, tapered to a point posteriorly; origin of spermathecal ducts occurring immediately adjacent to furcal membrane; three spermathecae, much longer than wide, saclike, wider apically than basally; spermathecal duct thickened basally, extremely narrowed basal to spermathecae; bifurcation of spermathecal sac duct with spermathecal ducts very close to furcal membrane; spermathecal sac short, 2.5 x furcal lengths; sac ovoid, longer than wide; 3/4x length of spermathecal sac duct. Figure 3. Jeanchazeauia nubilosus sp. nov., female habitus (MEI030203) (artistic rendering by J. Marie Metz). Etymology. Derived from the Latin nubilus , cloudy, and - osus , full of; referring to the dark wing infuscation of the male. Figure 4. Phylogenetic placement of Calophytus gen. nov. and Jeanchazeauia gen. nov. in Agapophytinae based on supermatrix analysis of DNA sequence data. Figure modified after Winterton et al. (2016 : fig. 3). Comments. Jeanchazeauia nubilosus sp. nov. exhibits remarkable sexual dimorphism in wing pattern, but little to no other differences in the body or head. The male has largely uniform dark infuscate wings, while the wings of the female are strongly banded. It is not known if this condition extends to other species in the genus where the males are unknown, and such dramatic sexual dimorphism in wing patterning is rare in stiletto flies to this degree. Jeanchazeauia nubilosus sp. nov. has been collected in dry to humid tropical forest at higher elevations on several mountains in New Caledonia (e.g., Figs 31A, C , 32B ). GenBank sequences for this species (see Winterton et al. 2016 : table S1): KM885007 (28S rDNA), KM879119 (EF1a). Figure 5. Lateral view of antennae of Calophytus gen. nov. A C. chazeaui sp. nov. B C. grandiosus sp. nov. C C. schlingeri sp. nov. D C. matilei sp. nov. E C. webbi sp. nov. (figures not to scale) (drawings by J. Marie Metz). Specimens examined. Holotype male, New Caledonia: Province Nord: Mt. Dzumac [Dumbea, approximated as -22.106 , 166.458 ], black light (UV), 27-28.II.1984, M. Pogue, M. Epstein (MEI030206, MNHN). Figure 6. Scutum and scutellum of Calophytus gen. nov. and Jeanchazeauia gen. nov. A C. schlingeri sp. nov. B C. grandiosus sp. nov. C C. matilei sp. nov. D C. webbi sp. nov. E J. nubilosus sp. nov. (figures not to scale) (drawings by J. Marie Metz). Figure 13. Calophytus matilei sp. nov. A adult male (MEI135018), oblique view B same, lateral view C adult female (MEI071892), oblique view D same, lateral view. Body length: male: 8.0 mm; female: 9.4 mm. Figure 14. Calophytus matilei sp. nov., cleared male genitalia A aedeagus, lateral view B gonocoxites, ventral view C epandrium. Scale bar: 0.2 mm. Figure 15. Calophytus monteithi sp. nov. A adult female (MEI138464), oblique view B same, lateral view. Body length: 10.3 mm. Paratypes . New Caledonia: Province Nord: 1 male, 13 km by road from Ouego[a] to Mont Mandjanie , Malaise trap along stream [ -20.374 , 164.482 ], 175 m, 26.XI.1992, D.W. Webb, E.I. & M. Schlinger, (MEI030207, CSCA). Province Sud: 1 male, Reserve Speciale de Botanique, Mount Ningua, Malaise trap [ -21.735 , 166.142 ], 1100 m, 12-21.XII.2000, L.J. Boutin, M.E. Irwin (MEI131365, CSCA); 3 females, Reserve Speciale de Botanique, Mount Ningua, Malaise trap [-21.735, 166.142], 1100 m, various dates: 9-21.XII.2000, D.W. Webb, E.I. Schlinger, M.E. Irwin (MEI131362-4, CSCA); 2 females, Ningua Reserve Camp, Malaise trap, rainforest [-21.75, 166.15], 1100 m, 12-13.XI.2001, 27.XI-29.I.2002, Burwell, Monteith (MEI138461-2, QM); 2 females, 17 km NNE Noumea , Mt. Koghis, Malaise trap in tropical forest [ -22.167 , 166.533 ], 500 m, 5-15.XI.1992, D.W. Webb (MEI030202-3, CSCA); 2 females, 17 km NNE Noumea , Mt. Koghis, Malaise trap across forest stream [ -22.167 , 166.533 ], 500 m, 23-26.XII.1991, M.E. Irwin, D.W. Webb (MEI030195-6, MEI); 1 female, Noumea , Mt. Koghis, Malaise trap [ -22.167 , 166.533 ], 500 m, 4.XII.1963, R. Straatman (MEI030197, BPBM); 1 female, 17 km NNE Noumea , Mount Khogis, Malaise trap across path in rainforest [ -22.176 , 166.505 ], 425 m, 8.I.1996, M.E. Irwin, D.W. Webb, E.I. Schlinger, (MEI071888, CSCA); 3 females, 30 km NW Yate, Riviere Bleue, Malaise trap [ -22.117 , 166.658 ], 12-25.XI.1986, 11-27.X.1988, L.B. de Larbogne, J. Chazeau, A. & S. Tillier, (MEI030198-200, MNHN); 1 female, Riviere Bleue Provincial Park, 19.6 km Riviere Bleue Road, Malaise trap across forest path [ -22.117 , 166.658 ], 183 m, 18-20.XI.1992, D.W. Webb (MEI030201, CSCA). Figure 16. Calophytus schlingeri sp. nov. A adult male (MEI030158), anterolateral view B same, oblique view C adult female (MEI030172), lateral view D same, oblique view. Body length: male: 8.6 mm; female: 9.8 mm. Figure 17. Calophytus schlingeri sp. nov., cleared male genitalia A epandrium B gonocoxites and aedeagus, ventral view C aedeagus, dorsal view D gonocoxites, ventral view. Scale bar: 0.2 mm. Figure 18. Calophytus schlingeri sp. nov., genitalia A epandrium B gonocoxites, ventral view C same, lateral view D aedeagus, lateral view E same, dorsal view F female genitalia, lateral view, internal structures shown. Scale line: 0.2 mm. Abbreviations: ag , accessory gland; d , distiphallus; da , dorsal apodeme of parameral sheath; ea , ejaculatory apodeme; c , cercus; A1 , primary acanthophorite spines; A2 secondary acanthophorite spines; ga , gonocoxal apodeme; gs , gonostylus; igp , inner gonocoxal process (articulated); lea , lateral ejaculatory apodeme; ogp , outer gonocoxal process; s8 , sternite VIII; s10 , sternite X; s , spermatheca; ss , spermathecal sac; ssd , spermathecal sac duct; sd , spermathecal duct; vl , ventral lobe. Figure 19. Calophytus webbi sp. nov. A adult male (MEI030179), lateral view B same, oblique view. Body length: 6.3 mm. Figure 20. Calophytus webbi sp. nov. adult female head, dorsolateral view. Figure 21. Calophytus webbi sp. nov., cleared male genitalia A epandrium and tergite VIII B gonocoxites and aedeagus, dorsal view with epandrium removed. Scale bar: 0.2 mm. Figure 22. Distribution of Calophytus spp. collecting records throughout New Caledonia. Provinces are labelled and ecoregions delineated by colour.