Hydnum khanspurense sp. nov. and H. berkeleyanum from Pakistan Author Ali, Muhammad Author Ghafoor, Aneeqa 0000-0002-4277-4317 Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan aneeqaghafoor12@gmail.com Author Ghafoor, Annie 0009-0000-9496-254X annieghafoor78@gmail.com Author Niazi, Abdul Rehman 0000-0002-1118-1148 Institute of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan drarniazi.botany@pu.edu.pk Author Swenie, Rachel A. 0000-0003-1559-1037 Department of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, Tennessee 37996, USA & Harvard University Herbaria, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA rswenie@fas.harvard.edu text Phytotaxa 2024 2024-07-18 660 2 161 170 http://dx.doi.org/10.11646/phytotaxa.660.2.6 journal article 302341 10.11646/phytotaxa.660.2.6 a15ac3b3-3785-43c4-8bfe-34b5ed7fd6ae 1179-3163 13218124 Hydnum khanspurense Niazi, Ghafoor, & M. Ali sp. nov. ( Figures 2–3 ) MycoBank no. MB854208 Etymology:— The species epithet ‘ Hydnum khanspurense’ (Latin) refers to the type locality of the specimen. Diagnosis:— This species is characterized by the fleshy, velutinous yellowish orange to pale orange plano-convex pileus, non-decurrent, conical light orange spines, central stipe, smaller broadly ellipsoid to obovoid basidiospores (7.02–8.87 × 5.51–8.03 µm) and basidia (32.31–49.13 × 5.55–8.61 µm) with 1–4 sterigmata. Holotype :— Pakistan , Khyber Pakhtunkhwa , Khanspur , 2250 m a.s.l. , under Pinus wallichiana A.B. Jacks. , solitary or gregarious on decomposing needles and soil, 08 August 2021 , Abdul Rehman Niazi & Aneeqa Ghafoor , KH-09 ( LAH88021 ). GenBank accession no: OQ130694. Basidiomes 22–61 mm high, fleshy. Pileus 20–42 × 18–38 mm , Surface velutinous yellowish orange (8/8 YR 10) to pale orange (8/3 YR 5), smooth, plano-convex, slightly depressed from center. Margin entire, straight, yellowish orange (8/8 YR 10). Context 2–4 mm wide, yellowish white. Spines 1.3–1.9 × 0.2–0.3 mm , conical, acute, light orange, crowded, slightly adnexed. Stipe 20–58 × 8–14 mm , white, smooth, central, cylindrical, slightly broader at the base and covered with white colored mycelium. Odour fruity. FIGURE 1. ITS phylogeny representing the relationships between Hydnum khanspurense sp. nov. , Hydnum berkeleyanum , and related taxa in Hydnum subgenera Pallida and Rufescentia based on Maximum Likelihood and Bayesian Inference. Bootstrap support values and posterior probabilities are shown adjacent to each node. Sequences representing the Pakistani specimens are indicated in bold. FIGURE 2. Macromorphology of basidiomata of Hydnum khanspurense (A–B, LAH88021–LAH29722), and H. berkeleyanum (C–D, LAH12817- LAH08818). Scale bar (A–D) = 1 cm. Basidiospores [40/3/3] (7.02) 7.12–8.74(8.87) × (5.51) 5.66–7.69 (8.03) µm, Qavl × Qavw = 7.86 × 6.65 µm, Q = 1.18, broadly ellipsoid to obovoid, thin walled, hyaline, smooth, with granular contents. Basidia 32.31–49.13 × 5.55– 8.61 µm, clavate to sometime narrowly clavate, clamps present at the base, with 1–4 sterigmata. Sterigmata 2.63–5.67 × 0.97–2.26 µm, Basidioles 21.66–47.11 × 4.26–13.37 µm, numerous clavate to subclavate, Hyphae present at apex of spines 4.53–6.97 µm wide, cylindrical, thin-walled, clamped. Pileipellis a cutis composed of hyphae 5.8– 10.2 µm wide, interwoven, cylindrical, clamped. Stipitipellis composed of hyphae 3.96–11.18 µm wide, interwoven, cylindrical, clamped. Habit and Habitat:— solitary to gregarious, growing on soil and decomposing needles of pine trees in pinedominated moist temperate forest of Northern Pakistan . Additional material Examined: Pakistan , Khyber Pakhtunkhwa , Helipad forest near Khanspur village, 2250 m a.s.l., under Pinus wallichiana , solitary and gregarious on soil and decomposing needles, 29 July 2022 , Abdul Rehman Niazi and Aneeqa Ghafoor, KH 50 ( LAH 29722). GenBank accession no: OQ130695. Pakistan , Khyber Pakhtunkhwa , Abbottabad district, Kuza Gali, 2452 m a.s.l., under Pinus wallichiana , 20 August 2022 Abdul Rehman Niazi and Aneeqa Ghafoor, KH-451 ( LAH 20822), GenBank accession number: OQ130696 Hydnum berkeleyanum K. Das, Hembrom, A. Baghela & Vizzini in Wang, Das, Horman, Antonin, Baghela, Chakraborty, Hembrom, Nakasone, Ortiz-Santana, Vizzini, Hofstetter & Buyck, Cryptog. Mycol. 39(2): 212 (2018) ( Figures 2–3 ) Pileus 20–55 mm diam., convex or planoconvex to plane, centrally depressed or umbilicate, surface dry, shiny, hygrophanous, small scales rarely present at the margin; orange-brown (5 YR 5/6) to bright reddish orange (10 R 5/12), or pale orange (9.5 R 5/12) towards the margin. Margin entire, slightly incurved turning straight to lobed or undulate with age. Context 2–4 mm wide, white, yellowing at the cut. Spine 3–5 × 0.4–0.7mm adnate, conical, acute, crowded, white to pale orange. Stipe 12–70 × 5–15 mm , central to eccentric, cylindrical or clavate, surface tomentose-pruinose, whitish, turning yellow on bruising or handling. FIGURE 3. Line Drawings of Hydnum khanspurense (A–D, LAH88021, LAH29722, LAH20822) and H. berkeleyanum (E–G, LAH12817–LAH08818). A–B. Basidia and basidioles; C. Basidiospores; D. Pileipellis; E. Basidia; F. Basidiospores; G. Pileipellis (Scale bar A–F=5μm). Basidiospores [30/2/2] (6.5–) 7.0–8.5(–9) × (6.0–) 6.5–7.5 (–8.0) µm, Qavl × Qavw = 7.84 × 6.98 µm Q =1.07, globose or subglobose, to ovoid, thin-walled, non-amyloid, hyaline, apiculate, Basidia 30–48.5 × 7.0–9.5 µm, clavate, 2–4 spored, with granular content, frequently clamped, Hyphae present at apex of spines 3.1–5.5 µm wide, cylindrical, thin-walled, Pileipellis a cutis of cylindrical, tightly interwoven, thin-walled hyphae 2.6–10 µm wide, clamped, with round to slightly tapering ends, Stipitipellis composed of cylindrical, parallel, thin walled, clamped hyphae, 3.0–5.0 µm broad with round ends. Habit and Habitat:— gregarious under Pinus wallichiana in mixed forests of Pakistan and temperate broadleaf forests of India ; also China . Additional material examined:— Pakistan , Khyber Pakhtunkhwa , Abbottabad district, Ayubia National Park, 2400 m a.s.1, 12 August, 2017, Muhammad Ali and Abdul Rehman Niazi, KH 373 ( LAH 12817) GenBank accession no: OQ130700. ibid. 08 August 2018 , Abdul Rehman Niazi and Muhammad Ali, KH 421 ( LAH 08818), GenBank accession number: OQ130701.