New records of water mites from the Balkans revealed by DNA barcoding (Acari, Hydrachnidia) Author Pešić, Vladimir Department of Biology, University of Montenegro, Cetinjski put b. b., 81000 Podgorica, Montenegro. E-mails: anamanovic @ yahoo. com; milicajovanovic 93 @ yahoo. com vladopesic@gmail.com Author Jovanović, Milica Department of Biology, University of Montenegro, Cetinjski put b. b., 81000 Podgorica, Montenegro. E-mails: anamanovic @ yahoo. com; milicajovanovic 93 @ yahoo. com Author Manović, Ana Department of Biology, University of Montenegro, Cetinjski put b. b., 81000 Podgorica, Montenegro. E-mails: anamanovic @ yahoo. com; milicajovanovic 93 @ yahoo. com Author Karaouzas, Ioannis Institute of Marine Biological Resources and Inland Waters, Hellenic Centre for Marine Research, 46.7 km Athens- Sounio Av., Anavyssos, 19013, Greece. E-mail: ikarz @ hcmr. gr Author Smit, Harry Naturalis Biodiversity Center, P. O. Box 9517, 2300 RA Leiden, the Netherlands. E-mail: Harry. Smit @ naturalis. nl * Corresponding author. E-mail: vladopesic @ gmail. com text Ecologica Montenegrina 2021 2021-12-22 49 20 34 journal article 10.37828/em.2021.49.2 2336-9744 8044105 Hygrobates longiporus Thor, 1898 Hygrobates longiporus is a widely distributed species in the Palaearctic, inhabiting various types of standing waters and pools of streams ( Gerecke et al . 2016 ). We successfully barcoded one specimen from Greece and Serbia , respectively, and two specimens from south-western Turkey , which match the description of H. longiporus . The final alignment for species delimitation using COI sequence data comprised sequences of 7 specimens morphologically assigned to H. longiporus and one specimen of H. meditterraneus (as outgroup). The final alignment consisted of 658 nucleotide positions. The neighbour-joining (NJ) tree is presented in Fig. 5 . Table 6. List of Hygrobates longiporus specimens used in this study. BINs are based on the barcode analysis from 10- 12-2021.

Locality (country, name)	Lat/Long	BOLD Acc.	Voucher code	BIN
Nos.
Hygrobates longiporus Thor, 1898
Norway, upstream of the bridge at old	58.0895 N, 7.83998 E	HYDCA284	NTNU-VM 227724	BOLD:AEB8359
Soegne church
Norway, Ved Blakstad, upstream of the	58.51 N, 8.648 E	HYDCA518	NTNU-VM 227721	BOLD:AEB8359
bridge Frolandsveien
Norway, Ved Blakstad, upstream of the	58.51 N, 8.648 E	HYDCA519	NTNU-VM 227722	BOLD:AEB8359
bridge Frolandsveien
Serbia, Crni Rzav near Vodice	43.6559 N, 19.704 E	CCDB 38363 D11	SEPTA047-21
Turkey, Kizilli Village, Bucak	37.3425 N, 30.9256 E	CCDB 38363 G05	SEPTA077-21
Turkey, Kizilli Village, Bucak	37.3425 N, 30.9256 E	CCDB 38363 G06	SEPTA078-21
Greece, Amygdalorema stream	41.1374 N, 25.5358 E	CCDB 38362 B01	SEPTB013-21
Hygrobates mediterraneus Pešić, 2020
Greece, Glaukos River	38.2008N, 21.7908E	CCDB 38363 H06	SEPTA090-21

We obtained two haplogroups of H. longiporus ( Fig. 5 ). The first highly supported clade (HygL-I) contains sequences from the Balkans ( Serbia and Greece ) and two sequences from Turkey . This clade is placed as the sister clade of specimens from Norway identified also as H. longiporus (HygL-2); the distance (24.3±2.2% K2P) between these clades is very high, suggesting long independent history of these two species. Hygrobates longiporus was originally described from Norway ( Thor 1898 ) and can be assumed that this species corresponds to Clade I-type in our analysis. The second clade in our analysis appears to be widespread in the Mediterranean and there is a possibility that this species corresponds to H. falcilaminatus Walter, 1926 . The latter species was originally described from Azrou, Morocco ( Walter 1926 ), reported from Spain and France , and later on synonymized with H. longiporus by Lundblad (1956) (see also Gerecke 1991 for a discussion about this species). Unfortunately, no sequence is available for H. falcilaminatus . Over the last two years, we have tried in collaboration with Youness Mabrouki ( Morocco ) to collect specimens of H. falcilaminatus from its locus typicus , unfortunately without success. This clade should be left unnamed until the sequences of the latter species are available.