Benzoylsalicylic acid isolated from seed coats of Givotia rottleriformis induces systemic acquired resistance in tobacco and Arabidopsis Author Kamatham, Samuel Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500 046, Telangana, India & Department of Biochemistry, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500 046, Telangana, India Author Neela, Kishore Babu Author Pasupulati, Anil Kumar Author Pallu, Reddanna Author Singh, Surya Satyanarayana Author Gudipalli, Padmaja text Phytochemistry 2016 2016-06-30 126 11 22 http://dx.doi.org/10.1016/j.phytochem.2016.03.002 journal article 10.1016/j.phytochem.2016.03.002 1873-3700 10484904 2.2. Detection of SA precursors in seed coats of G. rottleriformis The other compounds of fraction 3 were identified as BA that eluted at RT 14.7 min, salicylic acid (SA) at RT 15.3 min, benzaldehyde (BD) at RT 19.4 min, and CA at RT 21.4 min ( Fig. 1 and Supplementary Fig. S6a–d ). The purified BA and SA were also analysed by IR and NMR (Supplementary Figs. S7a and b and S8a–c). We also detected methyl salicylate (MeSA) and methyl benzoate (MeBA) in active fraction 3 (Supplementary Fig. S9). The conjugates of the SA and BA have already been reported previously (Chong et al., 2001; Lee et al., 1995 ). The biosynthesis of SA and its role in disease resistance has been established in various plants ( Meuwly et al., 1995 ; Ribnicky et al., 1998 ; Silverman et al., 1995 ; Vogt, 2010 ; Wildermuth et al., 2001 ). The precursors of SA pathway were identified as CA , BD, benzoyl-CoA and BA ( Boatright et al., 2004 ; Gao et al., 2015 ; Ribnicky et al., 1998 ). Though the biosynthesis of SA is well known in plants ( Ribnicky et al., 1998 ; Seyfferth and Tsuda, 2014 ; Wildermuth et al., 2001 ), identification of SA along with its precursors and its new derivative BzSA in the present study adds new information with respect to metabolism of SA in plants.