Description of the pupa and additional characters of the fourth-instar larva female, and male genitalia of Uranotaenia (Uranotaenia) coatzacoalcos Dyar & Knab, with keys for the identification of Mexican species of Uranotaenia (Diptera: Culicidae) Author Rivera-García, Karina D. Author Rísquez-Pérez, Abril Author Ibáñez-Bernal, Sergio text Zootaxa 2019 2019-05-20 4608 2 247 260 journal article 26774 10.11646/zootaxa.4608.2.3 c9304687-1140-4c3d-92ca-3a0cf433bb08 1175-5326 3055640 6CC4960C-FB3F-4F05-93AC-CF7B419A38D2 Uranotaenia ( Uranotaenia ) coatzacoalcos Dyar & Knab Uranotaenia ( Uranotaenia ) coatzacoalcos Dyar & Knab, 1906: 186 (larva). Type locality: Mexico , Veracruz , Santa Lucrecia. Additional references: Howard et al . 1912 : pl. 135, fig. 512 and pl. 138, fig. 538; Howard et al . 1917: 916 (larva); Dyar 1928: 424 (female, male, larva); Lane 1953: 572 (female, male, larva; includes Ur . typhlosomata Dyar & Knab and Ur. basalis Howard, Dyar & Knab as synonyms); Galindo et al . 1954: 136 (male, female, larva; resurrection of Ur . typhlosomata from synonymy). Uranotaenia basalis Howard, Dyar & Knab, 1917: 917 . Type locality: Mexico , [ Veracruz ], Córdoba (male, larva). Material examined . Mexico , Veracruz , municipality of Coatepec, Congregación Tapachapan, November 25, 2017 , 2 ♀ , 3 ³, 5 pupal exuviae, 5 larval exuviae (of the same specimens), 1 larva, tropical montane cloud forest ( 19° 27′ 18″ N 96° 58′ 49″ W ). Note: Ur . coatzacoalcos was found in artificial containers filled with colored turbid water with plant debris located at the periphery of a house in the periurban area of Coatepec. Immature stages were found in association with Lutzia bigoti (Bellardi) . The species was identified based on larval, adult female, and male genitalia characteristics (see Figs. 1 , 3 , 4 ); the respective pupal exuviae were used for description. Larva ( Fig. 1 a–f). Chaetotaxy in Table 1 . Head : Head capsule dark brown. Antenna : With small spicules; seta 1-A single, small, inserted before middle of antenna ( Fig. 1b ). Abdomen ( Fig. 1a ): Comb plate with 9 scales. Pecten with 15 spines that do not extend beyond mid-length of siphon; seta 1-S inserted near mid-length of siphon before last spine of pecten; siphon index about 3.7 ( Fig. 1c ). Saddle with strong spines along posterior margin that differ in size ( Fig. 1d ); saddle/siphon index about 1.75. Anal papillae longer than saddle, dorsal papillae slightly longer than ventral papillae ( Fig. 1c ). TABLE 1. Number of branches for setae of the fourth-instar larva of Uranotaenia ( Uranotaenia ) coatzacoalcos .

Seta	Head	Antenna	Thorax	Abdominal segments
no.	C	A	P	M	T	I	II	III	IV	V	VI	VII	VIII	X
0	1	–	7,8	–	–	–	1	1	1	1	1	1	0	–
1	1	1	1	6,7	4,5	1	4	5	4	4	4	4	7	5
2	–	1	1	1	3*	1	1,2	1	1	1	1	1	1	2
3	1	1	11,12	3	4,5	5	5	1	2,3*	1	3*	4	5	2
4	2,3	1	3	4*	5	6–8	5	2*	1	2,3	3*	1	1	5
5	1	1	1	1	1	2	3	2	1	1	1	6	6	–
6	1	1	1	1	1	2	2	3	4	4	5	6	1-S	10
7	6,7	–	3	1	6	1	1	4*	3*	4*	1	3*	2-S	1
8	2	–	6	6	10	–	1	2	3	2	3	8	–	–
9	3	–	3	6	4	1	1	1	1	1	1	4	–	–
10	1	–	2	1	1	2*	2*	2*	1	1	1	2	–	–
11	6	–	3	3	3	4–6	2*	2*	1	1	1	1	–	–
12	?	–	1	1	3*	2,3*	2*	3*	1	1	1	1	–	–
13	?	–	–	7	7	2*	5	6	6	5	16	6	–	–
14	?	–	15,16	10,11	–	–	–	1	1	1	1	1	1	–
15	1	–	–	–	–	–	–	–	–	–	–	–	–	–

* Number of terminal branches (forked seta).? Not observed. FIGURE 1. Uranotaenia ( Uranotaenia ) coatzacoalcos . Larva: a) Thorax and abdomen (segments I–VI); b) head; c) terminal part of abdomen (segments VII,VIII,X and siphon); d) siphon marginal spicules; e) comb scale; f) pecten spine. C, head; A, antenna; P, prothorax; M, mesothorax; T, metathorax; S, siphon; I–VIII,X, abdominal segments I–VIII,X; 0–14 = setal numbers on specified areas. Scales in mm. FIGURE 2. Uranotaenia ( Uranotaenia ) coatzacoalcos . Pupa: a) Cephalothorax; b) abdomen; c) female genital lobe; d) male genital lobe; e) paddle marginal serrations. CT, cephalothorax; Pa, paddle; Se, paddle marginal serrations; GL, genital lobe; Pr, proctiger; Ce, cercus; I–IX, abdominal segments I–IX; 0–14 = setal numbers on specified areas. Scales in mm. Pupa ( Fig. 2 a–e). Chaetotaxy in Table 2 . Cephalothorax : Lightly tanned to yellowish, darker in postscutal area. Trumpet : Cylindrical, moderately dark, tracheoid area dark. Length, ♀ 0.51/0.52 ( n = 2), ³ 0.49–0.52 ( n = 3; mean 5.1; sd 0.03); width, ♀ 0.08/0.09 ( n = 2), ³ 0.07–0.09 ( n = 3; mean 0.08; sd 0.01); trumpet index, ♀ 5.6/6.5 ( n = 2), ³ 5.7–7.4 ( n = 3; mean 6.44; sd 0.757;); pinna, ♀ 0.16/0.17 ( n = 2), ³ 0.13–0.16 ( n = 3; mean 0.14; sd 0.023); meatus, ♀ 0.35 ( n = 2), ³ 0.36–0.37 ( n = 3; mean 0.36; sd 0.003); tracheoid area about 2.3 from base ( n = 5; mean 2.16; sd 0.028) ( Fig. 2a ). Abdomen : Length, ♀ 2.55/2.75 ( n = 2), ³ 2.12–2.5 ( n = 3; mean 2.35; sd 0.63). Paddle : Inner and outer margins with strong serrations of different sizes from mid-length to apex ( Fig. 2e ). Midrib conspicuous, almost reaching apex. Length, ♀ 0.72/0.75 ( n = 2), 0.71–0.73 ( n = 3; mean 0.72; sd 0.01); width, ♀ 0.5 ( n = 2), ³ 0.48–0.5 ( n = 3; mean 0.49; sd 0.01); paddle index, ♀ 1.44/1.5 ( n = 2), ³ 1.42–2 ( n = 3; mean 1.63; sd 0.1). Genital lobe : Slightly yellowish, with conspicuous spicules in both sexes. Length, ♀ 0.1/0.11 ( n = 2) ( Fig. 2c ), 0.19–0.2 ( n = 3; mean 0.2; sd 0.01) ( Fig. 2d ). TABLE 2. Number of branches for setae of the pupa of Uranotaenia ( Uranotaenia ) coatzacoalcos . Modes in parentheses.

Seta	Cephalothorax	Abdominal segments		Paddle
no.	CT	I	II	III	IV	V	VI	VII	VIII	IX	Pa
0	–	–	1	1	1	1	1	1	1	–	–
1	2,3(2)	12–16+	8–13(13)*	5–8(7)	3–6(4)	2,3(3)	1–3(2)	1,2(1)	–	1	1
2	2	1	1	1	1	1	1	1	–	–	–
3	2	1,2(2)*	2,3*	2,3(2)*	2,3(3)*	1,2(1)*	1	1–3(2)	–	–	–
4	2,3(3)	3,4*	2–4(3)*	1,2(2)*	1,2(2)*	1–4*	1–3*	1	1	–	–
5	2–4(3)	2–4(3)*	2-4(3)	3–7(4)	2–4(3)	2,3(2)	1–3(2)	2	–	–	–
6	1	1	1	1,2(1)	1	1,2(1)	1,2(1)	1,2(1)*	–	–	–
7	1,2(2)	2*	2,3(2)*	1–3(2)*	1,2(2)*	2–4(3)*	1	1	–	–	–
8	3,4(4)	–	–	1-3(2)*	3,4(3)*	2–4(3)*	2–4(3)*	1,2(2)*	–	–	–
9	2–4(2)*	1,2(1)*	1	1	1	1	1	1,2(2)*	3–6(4)	–	–
10	3,4(3)	?	–	1	1	1	1	1	–	–	–
11	2,3(2)	?	–	1	1	1	1	1	–	–	–
12	2,3(2)	–	–	–	–	–	–	–	–	–	–
14	–	–	–	1	1	1	1	1	1	–	–

* Number of terminal branches (forked seta). + Number of basal branches. ? Not observed. Female ( Fig. 3b,c ). Medium-sized mosquito, wing length 2.9–3.0 mm. Head : Proboscis dark brown, about 0.9 length of forefemur, swollen before apex. Maxillary palpus short with dark-brown scales on all surfaces. Clypeus nude. Occiput clothed with large dark-brown flat scales. Vertex and ocular line with silvery-white scales. Thorax : Antepronotum with conspicuous patch of silvery-white scales. Dorsocentral setae very large. Scutum covered with dark to dark-brown scales, with a conspicuous line of silvery-white scales from scutal angle to base of wing. Pleura dark-brown with a line of silvery-white scales extending nearly horizontal on mesokatepisternum. Femora with silvery-white scales on ventral surface. Femora and tibiae with conspicuous spots of silvery-white scales at apices. Midtibia with a patch of silvery-white scales on apical part (0.12 of total length of midtibia). Foretarsus completely covered with dark-brown scales. Midtarsomeres 1 and 2 with dark-brown scales; 3–5 with silvery-white scales. Hindtarsomeres 4, 5, and 0.45 of 3 with silvery-white scales, other tarsomeres with dark-brown scales. Wing with dark scales except for a line of silvery-white scales along radial vein from base to mid-length (before the fork). Abdomen : Tergum I covered with silvery-white scales except for small lateral patches of dark scales. Terga II–VII with a U-shaped basal patch of silvery-white scales in central part (approximately 0.45 of each segment), rest of the terga with dark scales. Male ( Figs 3a , 4 a–c). Body coloration and characteristics similar to female. Wing length 2.4–2.6 mm ( Fig. 3a ). Genitalia : Tergum IX with proximal margin deeply emarginate, subquadrate; plate transversally directed with a pair of inconspicuous nude thumb-like lobes at distal margin which are widely separated at middle by a shallow nearly straight interlobular membranous margin ( Fig. 4c ). Gonocoxite conical, length about 1.5 basal width, with a poorly developed basal mesal lobe bearing a group of non-specialized setae that are somewhat larger than those nearby. Gonostylus short, about 0.5 length of gonocoxite, narrow at middle, ending in a rounded apical knob with one small gonostylar claw, and with patch of short spicules ( Fig. 4a ). Proctiger triangular in dorsal view, membranous and nude. Lateral plate of aedeagus heavily sclerotized, tergal arm with blunt apex and a preapical long external projection, sternal arm with 5 small teeth, all anteriorly directed; aedeagus short, lanceolate, laterally directed; parameres well developed, with blunt apex reaching level of apex of aedeagus ( Fig. 4b ).