Twenty-two new species in the genus Hyphantrophaga Townsend (Diptera: Tachinidae) from Area de Conservacion Guanacaste, with a key to the species of Mesoamerica Author Fleming, AJ Author Wood, D. Monty Author Smith, M. Alex Author Dapkey, Tanya Author Hallwachs, Winnie Author Janzen, Daniel text Biodiversity Data Journal 2019 7 29553 29553 http://dx.doi.org/10.3897/BDJ.7.e29553 journal article http://dx.doi.org/10.3897/BDJ.7.e29553 1314-2828-7-e29553 Hyphantrophaga anacordobae Fleming & Wood sp. n. Materials Type status: Holotype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007484 ; recordedBy: D.H. Janzen, W. Hallwachs & Freddy Quesada ; individualID: DHJPAR0007484; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT256-06, 02-SRNP-23842, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 04-Nov-2002 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007481 ; recordedBy: D.H. Janzen, W. Hallwachs & gusaneros ; individualID: DHJPAR0007481; individualCount: 1 ; sex: female ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT253-06, 95-SRNP-10486, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Santa Maria; locality: Area de Conservacion Guanacaste ; verbatimLocality: Estacion Santa Maria; verbatimElevation: 840 ; verbatimLatitude: 10.7645; verbatimLongitude: -85.3116; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.7645 ; decimalLongitude: -85.3116 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 16-Nov-1995 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007482 ; recordedBy: D.H. Janzen, W. Hallwachs & Mariano Pereira ; individualID: DHJPAR0007482; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT254-06, 02-SRNP-23679, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 14-Nov-2002 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007483 ; recordedBy: D.H. Janzen, W. Hallwachs & Mariano Pereira ; individualID: DHJPAR0007483; individualCount: 1 ; sex: female ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT255-06, 02-SRNP-23680, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 11-Oct-2002 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007480 ; recordedBy: D.H. Janzen, W. Hallwachs & gusaneros ; individualID: DHJPAR0007480; individualCount: 1 ; sex: female ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT252-06, 95-SRNP-10486.1, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Santa Maria; locality: Area de Conservacion Guanacaste ; verbatimLocality: Estacion Santa Maria; verbatimElevation: 840 ; verbatimLatitude: 10.7645; verbatimLongitude: -85.3116; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.7645 ; decimalLongitude: -85.3116 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 19-Nov-1995 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007485 ; recordedBy: D.H. Janzen, W. Hallwachs & Guillermo Pereira ; individualID: DHJPAR0007485; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT257-06, 02-SRNP-23678, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 16-Nov-2002 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007486 ; recordedBy: D.H. Janzen, W. Hallwachs & Freddy Quesada ; individualID: DHJPAR0007486; individualCount: 1 ; sex: female ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT258-06, 02-SRNP-24540.7, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 28-Jan-2003 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007487 ; recordedBy: D.H. Janzen, W. Hallwachs & Harry Ramirez ; individualID: DHJPAR0007487; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT259-06, 02-SRNP-24536.7, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 31-Jan-2003 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007488 ; recordedBy: D.H. Janzen, W. Hallwachs & Mariano Pereira ; individualID: DHJPAR0007488; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT260-06, 02-SRNP-24200, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 11-Dec-2002 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0007489 ; recordedBy: D.H. Janzen, W. Hallwachs & Mariano Pereira ; individualID: DHJPAR0007489; individualCount: 1 ; sex: female ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAT261-06, 02-SRNP-24542, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Cacao; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Ponderosa; verbatimElevation: 1060 ; verbatimLatitude: 10.9146; verbatimLongitude: -85.4626; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9146 ; decimalLongitude: -85.4626 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 21-Feb-2003 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0019597 ; recordedBy: D.H. Janzen, W. Hallwachs & Roster Moraga ; individualID: DHJPAR0019597; individualCount: 1 ; sex: female ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASTAB145-07, 07-SRNP-21398, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Del Oro; locality: Area de Conservacion Guanacaste ; verbatimLocality: Monte Cristo; verbatimElevation: 525 ; verbatimLatitude: 11.0137; verbatimLongitude: -85.4253; verbatimCoordinateSystem: Decimal; decimalLatitude: 11.0137 ; decimalLongitude: -85.4253 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphojustitiae ; verbatimEventDate: 26-May-2007 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0040955 ; recordedBy: D.H. Janzen, W. Hallwachs & Carolina Cano ; individualID: DHJPAR0040955; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ASHYF870-11, 10-SRNP-4655, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector San Cristobal; locality: Area de Conservacion Guanacaste ; verbatimLocality: Tajo Angeles; verbatimElevation: 540 ; verbatimLatitude: 10.8647; verbatimLongitude: -85.4153; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.8647 ; decimalLongitude: -85.4153 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2017; Event: samplingProtocol: Reared from the larva of the Nymphalidae, Morphoamathonte ; verbatimEventDate: 24-Nov-2010 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0059877 ; recordedBy: D.H. Janzen, W. Hallwachs & Manuel Rios ; individualID: DHJPAR0059877; individualCount: 1 ; sex: male ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ACGBA6298-16, 16-SRNP-31601, BOLD:AAC5448; Taxon: scientificName: Hyphantrophagaanacordobae; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Hyphantrophaga; specificEpithet: anacordobae; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Guanacaste; county: Sector Pitilla; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Cuestona; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2107; Event: verbatimEventDate: 09-Oct-2016 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Description Male (Fig. 4). Length: 9-12 mm. Head (Fig. 4b): vertex 1/4 of head width; two pairs of reclinate upper orbital setae; ocellar setae arising behind anterior ocellus; ocellar triangle black medially, brassy/silver laterally; fronto-orbital plate shiny silver and densely setulose, setulae not extending below lowest frontal seta; parafacial silver and bare; eye densely haired; facial ridge bare; pedicel black, concolorous with postpedicel; arista dark brown, very minutely pubescent, distinctly thickened on basal 1/3-1/4; palpus yellow and densely haired so as to appear darkened, apically with a slight clubbed appearance. Thorax (Fig. 4a, c): slightly brassy tomentose dorsally, contrasting with dark silver grey laterally; densely covered in black setulae along all surfaces, interspersed amongst setae; four thick and prominent dorsal vittae, outermost two broken across suture, innermost pair unbroken, reaching just beyond 2nd postsutural dorsocentral seta; postpronotum with 5-6 setae arranged in a triangle; chaetotaxy: acrostichal setae 3:3; dorsocentral setae 3:4; intra-alar setae 2-3:3; supra-alar setae 2:3; three katepisternal setae; basal scutellar setae subequal to slightly shorter than subapical scutellar setae; lateral scutellar setae subequal in length to apical setae, with a strong inward curve medially; apical scutellar setae 1/2 as long as subapical scutellar setae, crossed apically; one pair of discal scutellar setae set as widely apart as subapical setae; scutellum darkened across basal 20%, remainder concolorous with scutum. Legs (Fig. 4c): ground colour black; fore femur with dense silver tomentum on posterodorsal surface; hind coxa bare. Wing (Fig. 4a): pale translucent, hyaline, not distinctly infuscate; vein R4+5 with 3-4 setulae at base. Abdomen (Fig. 4a, c): ground colour dark reddish-brown, appearing black; middorsal depression on ST1+2 almost reaching hind margin; median marginal setae absent on ST1+2, present on T3; complete row of marginal setae present on T4; discal setae only on T5; sex patch covering 50% of the laterodorsal surface of T4 and the ventral surfaces of T4-T5; distinct brassy tomentose bands along anterior edge of T3 and T4, broken medially by a dorsocentral stripe and covering almost 40% of tergites; T5 with brassy tomentum throughout. Terminalia (Fig. 4d, e, f): sternite 5 (Fig. 4f) with a deeply excavated median cleft, squared off U-shaped, margins covered in dense tomentum. Lateral lobes of sternite rounded triangular apically, with 2-3 strong setae surrounded by many shorter, weaker setulae. Anterior plate of sternite 5 subequal to length of apical lobes; unsclerotised "window" slightly rectangular, two palmlike arms extending as wide as median cleft. Lateral lobe of sternite slightly pointed apically and with many short setae of equal length. Cerci in posterior view (Fig. 4d) slightly triangular and subequal in length to surstyli, blunt and rounded at apex, completely separate medially, appearing but not divergent; in lateral view with a strong downward angle commencing at midpoint; densely setulose along basal 2/3 dorsally, setulose ventrally along entire length (visible in lateral view). Surstylus in lateral view (Fig. 4e) almost parallel-sided along its length, ending in a slightly downcurved apex, making the structure appear blade-like; surstylus appearing to be fused with epandrium; when viewed dorsally surstyli appearing concave with a bulbous base where surstyli meet epandrium, inner margin straight slightly divergent at apices. Pregonite short, 0.5 times as long as distiphallus, bare and rounded off apically. Postgonite slightly narrow, 1/3 as wide as pregonite, sharply pointed and curved, vaguely C-shaped. Distiphallus ~1.5 times as long as basiphallus. Distiphallus sail-shaped, with a slender median longitudinal sclerotised reinforcement on its posterior surface and a broad, anterolateral, sclerotised acrophallus on each side, joining the plate of opposite side on anterior surface near apex. Female. Length: 8-11 mm. As male, differing only by the presence of two pairs of proclinate orbital setae. Diagnosis Hyphantrophaga anacordobae sp. n. can be distinguished from all other Hyphantrophaga species by the following combination of traits: three katepisternal setae, legs entirely black, hind coxa bare, ground colour of abdomen dark reddish-brown (appearing black under certain angles of light) and median marginal setae absent from ST1+2. Etymology Hyphantrophaga anacordobae sp. n. is named in recognition of Anabelle Cordoba Aleman's dedication and work in finding and rearing the ACG caterpillars that contained tachinid larvae. Distribution Costa Rica, ACG, Guanacaste Province, 525-1060 m elevation. Ecology Hyphantrophaga anacordobae sp. n. has been reared 13 times from two species of Lepidoptera in the family Nymphalidae , Morpho helenor (Cramer, 1776) and Morpho justitiae (Salvin & Godman, 1868), in rain forest, cloud forest and dry-rain lowland intergrade.