Revisiting the taxonomy and molecular systematics of Sesamia stemborers (Lepidoptera: Noctuidae: Apameini: Sesamiina): updated classification and comparative evaluation of species delimitation methods Author Hévin, Noémie M. C. 0000-0003-1730-0010 CBGP, INRAE, IRD, CIRAD, Institut Agro, Univ. Montpellier, Montpellier, France Author Kergoat, Gael J. 0000-0002-8284-6215 CBGP, INRAE, IRD, CIRAD, Institut Agro, Univ. Montpellier, Montpellier, France Author Zilli, Alberto 0000-0002-3416-8069 Institut des Sciences de l’Evolution de Montpellier, CNRS, IRD, EPHE, Université de Montpellier, 34095 Montpellier, France Author Capdevielle-Dulac, Claire Natural History Museum, Life Sciences, DC 2 - 2 N, Cromwell Road, SW 7 5 BD, London, UK Author Musyoka, Boaz K. Laboratoire Evolution Génomes Comportement et Ecologie, UMR CNRS 9191, IRD 247 and Université Paris Sud, Université Paris-Saclay, France Author Sezonlin, Michel Unité de Recherche UMR 247, African Insect Science for Food and Health (icipe), PO Box 30772 - 00100, Nairobi, Kenya Author Conlong, Desmond Département de Zoologie et de Génétique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin & South African Sugarcane Research Institute, Private Bag X 02, Mount Edgecombe, 4300, Republic of South Africa Author Van Den Berg, Johnnie School of Biological and Conservation Sciences, University of Kwazulu – Natal, Private Bag X 01, Scottsville, Pietermaritzburg, Republic of South Africa Author Ndemah, Rose School of Environmental Sciences and Development, North West University (Potchefstroom Campus), Private Bag X 6001, Potchefstroom, 2520 – Republic of South Africa Author Le Gall, Philippe International Institute of Tropical Agriculture, PO Box 2008 – Messa, Yaoundé, Cameroon Author Cugala, Domingos Eduardo Mondlane University, Faculty of Agronomy and Forestry Engineering, Av. J. Nyerere, Campus Universitario 1, Maputo, Republic of Mozambique Author Nyamukondiwa, Casper 0000-0002-0395-4980 Eduardo Mondlane University, Faculty of Agronomy and Forestry Engineering, Av. J. Nyerere, Campus Universitario 1, Maputo, Republic of Mozambique Author Pallangyo, Beatrice Department of Biology and Biotechnological Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye, Botswana. E-mail: nyamukondiwac @ biust. ac. bw Author Njaku, Mohamedi Department of Biology and Biotechnological Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye, Botswana. E-mail: nyamukondiwac @ biust. ac. bw Author Goftishu, Muluken 0000-0002-7194-9470 Biocontrol Program, PO Box 30031, Kibaha, Tanzania Author Assefa, Yoseph School of Plant Sciences, Haramaya University, P. O. Box 138, Diredawa, Ethiopia Author Kandonda, Onésime Mubenga Department of Crop Production, University of Swaziland, Swaziland Author Bani, Grégoire Faculté des Sciences agronomiques, Université de Kisangani, Kisangani, Democratic Republic of the Congo Author Molo, Richard Centre de Recherches Agronomiques de Loudima (CRAL), BP 28 – Loudima, Republic of the Congo Author Chipabika, Gilson Namulonge Agricultural and Animal Production Research Institute (NAARI), PO Box 7084 – Kampala, Uganda Author Ong’amo, George Zambia Agriculture Research Institute, Mount Maluku Central Research Station, PO Box 8 – Chilanga, Zambia Author Clamens, Anne-Laure School of Biological Science, College of Physical and Biological Sciences (Chiromo Campus), University of Nairobi, PO Box 30197, Nairobi, Kenya Author Barbut, Jérôme De ́ partement Syste ́ matique et Evolution, Entomologie, Muse ́ um National d’Histoire Naturelle, Paris, France Author Le Ru, Bruno Natural History Museum, Life Sciences, DC 2 - 2 N, Cromwell Road, SW 7 5 BD, London, UK & Laboratoire Evolution Génomes Comportement et Ecologie, UMR CNRS 9191, IRD 247 and Université Paris Sud, Université Paris-Saclay, France text Arthropod Systematics & amp; Phylogeny 2024 2024-06-13 82 447 501 journal article 10.3897/asp.82.e113140 25470FD2-80E2-4849-A9EC-C97FB6514182 Sesamia kalale Le Ru sp. nov. Figures 2 I – J ; 3 C, J ; 6 Type material. Holotype ♂ , ZAMBIA , Occidental Province , Kaoma , Kalale , 14 ° 47 ′ 55 ″ S , 25 ° 19 ′ 16 ″ E , 1154 m a. s. l. , III. 2012 , ex light trap ., gen. prep. LE RU Bruno / 114 , ( B. Le Ru leg.) ( MNHN ). Diagnosis. (See also the identification key of calamistis species subgroup, section 3.32). This species can be distinguished from the other known members of the calamistis subgroup by the combination of the following characters of the male genitalia: vinculum v-shaped at the outer margin, w-shaped at the inner margin without indentation, with a medium large saccus; apical extension of the sacculus as long as the cucullus, tapered and pointed at apex; juxta large and trapezoidal, the inferior plate v-shaped, the sides pointed, the superior plate wide and short, with blunted ends; phallus short and thick; vesica with a small flat triangular cornutus. Description. (Fig. 2 I, J ). Antenna ochraceous, shortly bipectinate at base and serrate at apex, flagellum adorned dorsally with ochraceous scales; palpus ochraceous; eyes brown. Head and thorax covered with light buff hairs. Forelegs of male ochraceous, otherwise light buff suffused with fuscous scales. Forewing light ochraceous heavily suffused with dark ochraceous and fuscous scales; three dark brown spots, one antemedial and one postmedial below the cell, one at apex of cell; a longitudinal fuscous fascia along lower margin of cell, partly within, partly without cell from base of cell to the subterminal line; outer margin adorned with brown spots between the veins, fringe light buff. Hindwing white, fringe concolor (white), basal line of fringe buff. Underside of forewing light buff, heavily suffused with ochraceous and fuscous scales, fringe buff suffused with fuscous scales; underside of hindwing white suffused with ochraceous and fuscous scales in costal area, fringe concolor (white). — Forewing length : male 22 mm (N = 1). — Male genitalia (Fig. 3 C, J ). Tegumen with large peniculi; vinculum v-shaped at the outer margin, w-shaped at the inner margin without indentation, with a medium large saccus. Valve with sacculus and cucullus separate; costa short and narrow, heavily sclerotized, ending with a long curved spine, with an apical tooth; sacculus heavily sclerotized rounded at base, a narrow and elongated apical extension as long as the cucullus, slightly curved inwards, adorned outwards with rows of short stout spines, pointed at apex; cucullus long, weakly sclerotized, very clavate at apex, with scattered and papillated hairs; juxta large and trapezoidal, the inferior plate v-shaped, the sides pointed, the superior plate wide and short, with blunted ends; uncus angled and stout at base, narrowed in distal part, pointed at apex, tufted with long hairs on upper side; phallus short and thick, dilated at base; lamina ventralis with an elongate carinal crest; vesica with a small flat triangular cornutus. Etymology. Named after Kalale a small village in the Occidental Province of Zambia ; treated as a noun in apposition. Distribution. Zambia . Known from one locality only in ‘ wetter Zambezian miombo woodland (dominated by Brachystegia Benth. , Julbernardia Pellegr. and Isoberlinia Craib & Stapf ) ’ (Mosaic # 25) vegetation mosaic ( White 1983 ) (Fig. 6 ) belonging to the Zambezian bioregion (sensu Linder et al. 2012 ). Ecology. Unknown; the only known specimen was caught with a light trap along the banks of a small river.