The identity of Argyria lacteella (Fabricius, 1794) (Lepidoptera, Pyraloidea, Crambinae), synonyms, and related species revealed by morphology and DNA capture in type specimens Author Landry, Bernard https://orcid.org/0000-0002-6005-1067 Museum d'histoire naturelle de Geneve, C. P. 6434, CH- 1211, Geneva 6, Switzerland bernard.landry@ville-ge.ch Author Bilat, Julia Museum d'histoire naturelle de Geneve, C. P. 6434, CH- 1211, Geneva 6, Switzerland Author Hayden, James https://orcid.org/0000-0003-1802-1064 Florida Department of Agriculture and Consumer Services, Division of Plant Industry, Entomology Section, 1911 SW 34 th Street, Gainesville, Florida, 32608, USA Author Solis, M. Alma https://orcid.org/0000-0001-6379-1004 Systematic Entomology Laboratory, Beltsville Agriculture Research Center, Agricultural Research Service, U. S. Department of Agriculture, c / o National Museum Natural History, MRC 168, Smithsonian Institution, P. O. Box 37012, Washington, District of Columbia, 20013 - 7012, USA Author Lees, David C. https://orcid.org/0000-0002-7580-8560 Natural History Museum, Cromwell Road, London, SW 7 5 BD, UK Author Alvarez, Nadir https://orcid.org/0000-0002-0729-166X Museum d'histoire naturelle de Geneve, C. P. 6434, CH- 1211, Geneva 6, Switzerland & Departement de Genetique et Evolution, Universite de Geneve, 30 quai Ernest Ansermet, 1205, Geneva, Switzerland & Museum cantonal des sciences naturelles, Palais de Rumine, Place de la Riponne 6, 1005, Lausanne, Switzerland Author Leger, Theo https://orcid.org/0000-0001-7330-3940 Museum fuer Naturkunde, Leibniz-Institut fuer Evolutions- und Biodiversitaetsforshung, Invalidenstr. 43, 10115, Berlin, Germany Author Gauthier, Jeremy https://orcid.org/0000-0001-6666-1002 Museum d'histoire naturelle de Geneve, C. P. 6434, CH- 1211, Geneva 6, Switzerland & Departement de Genetique et Evolution, Universite de Geneve, 30 quai Ernest Ansermet, 1205, Geneva, Switzerland text ZooKeys 2023 2023-02-07 1146 1 42 http://dx.doi.org/10.3897/zookeys.1146.96099 journal article http://dx.doi.org/10.3897/zookeys.1146.96099 1313-2970-1146-1 F9860399D28142A6B703C4115E01DDA1 BBF4F3B160DD5F48A78BED151A1E73D6 Argyria diplomochalis Dyar, 1913 Figs 9 , 14 , 19 , 20 , 25 , 31 , 35 Argyria diplomochalis Dyar, 1913: 113. Type locality: [USA] Culebra Island, Puerto Rico. Bleszynski and Collins 1962 : 213; Kimball 1965 : 234, US specimens misidentified; Bleszynski 1967 : 96; De la Torre y Callejas 1967 : 20; Alayo and Valdes 1982 : 61; Munroe 1995 : 35. Argyria diplamachalis [sic]: Schaus, 1940: 400. Type material examined. Lectotype ♂ (Fig. 9 ), here designated, with label data as follows: 1- "CulebraI[sland] | Feb[ruary]1899", 2- "PortoRico | Aug[ust.] Busck", 3- "Type | No.16245" | U.S.N.M.", 4- "Argyria | diplomochalis | Type Dyar", 5- "♂ genitalia | slide, 27 Apr[il].[19]'32 | C.H. #11.", 6- "Genitalia Slide | By CH | 107,449 | USNM", 7- "Lectotype | Argyria | diplomochalis Dyar, 1913 | Des[ignated] by M.A. Solis, 2022", deposited in the NMNH. Paralectotypes (8 ♂, 1 ♀), here designated with label data as follows: 3 ♂♂: 1-"Culebra I[sland] | Feb[bruary]1899", 2- "Porto Rico | Aug[ust] Busck"; 3 ♂♂: 1-"Bayamon | Jan[uary]1899"; 2- "Porto Rico | Aug[ust] Busck"; 1 ♀: 1-"Bayamon | Jan[uary]1899", 2- "Porto Rico | Aug[ust] Busck", 3-"GS-5620-SB | Argyria ♀ | lusella Z[eller] | det. Bleszynski , 19", 4-"Genitalia slide | By SB ♀ | USNM 52861"; deposited in NMNH. 1 ♂ [abdomen in vial]: 1- "SYN-TYPE" , 2- "Bayamon | Jan 1899", 3- "Porto Rico | Aug[ust] Busck", 4- "Bleszynski | Collection | B.M. 1974-309", 5- "Argyria | lusella Z[eller]. | ♂ | det. Bleszynski" , 6- "SYNTYPE | Argyria | diplomochalis | Dyar | det. M. Shaffer, 1975", 7- "NHMUK013697137" ; 1 ♂: 1- "SYN-TYPE" , 2- "Culebra I[sland]. | Feb 1899", 3- "Porto Rico | Aug[ust] Busck", 4- "Bleszynski | Collection | B.M. 1974-309", 5- "SYN-TYPE" | Argyria | diplomochalis | Dyar | det. M. Shaffer, 1975", 6- "NHMUK013697138" ; deposited in the NHMUK. Other specimens examined. 41 (see Suppl. material 2). Morphological diagnosis. Measuring 10-13 mm in wingspan this species (Figs 9 , 14 ) is quite similar in size and forewing markings to A. gonogramma (Figs 8 , 12 ), but it lacks a median line on the forewing and the median marginal markings are reduced to a faint brown bar on costa and a small dark-brown spot on the dorsal margin; the forewing costa and the head also appear more strongly marked (Fig. 31 ), notably more thickly dark brown at the base of the costa, on the frons laterally and on the labial and maxillary palpi; the costa of the forewing is also gold yellow to the apex, following the dark brown base. In male genitalia (Figs 19 , 20 ), this species differs most noticeably from the others treated here in the thicker and less strongly bent apical section of the gnathos and in the short valva with a prominent sickle-shaped projection at its base. In female genitalia (Fig. 25 ), A. diplomochalis differs from the others treated here more noticeably by the long and narrow ductus bursae without sclerotised section as well as in the large, circular corpus bursae; the ostium region also lacks any sclerotised 'pockets' . Figure 19. Male genitalia of Argyria diplomochalis . Holotype (NMNH). Figure 20. Male genitalia of Argyria diplomochalis . Specimen from St Croix Island (MHNG-ENTO-91929) ( A ) with phallus detached ( B ). Molecular results. The phylogenetic clade corresponding to the species A. diplomochalis comprises only three samples sequenced for this study. It appears that no sequence available in the BOLD database corresponds to this species. All three species delimitation approaches identify this clade (Fig. 1 ) and the average genetic divergence with the two closest species, A. insons and A. centrifugens , are respectively 9.25% and 7.16%, which confirms the high divergence of this clade and its specific status. Within this species, the GMYC and PTP approaches separate CRA04 on one hand and CRA05 and CRA06 on the other; and a mean divergence of 2.87% is observed between the samples of this clade. But this divergence may be related to a geographical divergence since CRA04 comes from the island of Anguilla and CRA05-CRA06 come from the US Virgin Island of Saint Croix. The integration of a larger number of samples in a genetic study could allow a finer molecular characterisation of this species. Distribution. Antilles, from Cuba in the West to Dominica in the Lesser Antilles in the east (Fig. 35 ). Remarks. Described from 12 cotypes from Culebra Island and Bayamon, Puerto Rico, a lectotype is designated here to ensure that the name continues to refer to this species exclusively. Dyar (1913) stated "Cotypes, 12 specimens", but only seven specimens were found at the NMNH while two others (now paralectotypes) are in the NHMUK. Examination of specimens of " A. diplomochalis " cited by Kimball (1965) , including ones in the FSCA labelled "5958,1" ( Kimball's number for that species), are A. gonogramma with scattered honey-brown scales on the forewings. This may be melanism caused by pupation during cold weather; all the specimens have been collected in winter or early spring.