Aspergillus capibaribensis (Eurotiales, Trichocomaceae), a new species in section Cremei Author Felipe, Maria Tamara C. 0000-0002-1692-5476 Departamento de Micologia Prof. Chaves Batista, Centro Acadêmico do Recife, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s / n, Centro de Biociências, Cidade Universitária, Recife, Pernambuco, CEP: 50670 - 901, Brazil mariatcfelipe@gmail.com Author Barbosa, Renan N. 0000-0003-2659-9157 Departamento de Micologia Prof. Chaves Batista, Centro Acadêmico do Recife, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s / n, Centro de Biociências, Cidade Universitária, Recife, Pernambuco, CEP: 50670 - 901, Brazil renan.rnb@gmail.com Author Bezerra, Jadson D. P. 0000-0002-7782-8602 Laboratório de Micologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235, s / n, Setor Universitário, Goiânia, Goiás, CEP: 7460505, Brazil jadsonbezerra@ufg.br Author Magalhães, Oliane M. C. 0000-0001-7275-5304 Departamento de Micologia Prof. Chaves Batista, Centro Acadêmico do Recife, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s / n, Centro de Biociências, Cidade Universitária, Recife, Pernambuco, CEP: 50670 - 901, Brazil oliane.magalhaes@ufpe.br Author Paiva, Laura M. 0000-0001-9720-9887 Departamento de Micologia Prof. Chaves Batista, Centro Acadêmico do Recife, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s / n, Centro de Biociências, Cidade Universitária, Recife, Pernambuco, CEP: 50670 - 901, Brazil mesquitapaiva@terra.com.br Author Souza-Motta, Cristina M. 0000-0002-0964-8271 Departamento de Micologia Prof. Chaves Batista, Centro Acadêmico do Recife, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s / n, Centro de Biociências, Cidade Universitária, Recife, Pernambuco, CEP: 50670 - 901, Brazil cristina.motta@ufpe.br text Phytotaxa 2024 2024-10-01 666 2 121 134 https://doi.org/10.11646/phytotaxa.666.2.3 journal article 10.11646/phytotaxa.666.2.3 1179-3163 14518853 Aspergillus capibaribensis M.T.C. Felipe, J.D.P. Bezerra, R.N. Barbosa & C.M. Souza-Motta sp. nov. ( Fig. 2 ) MycoBank no.: MB849455 Etymology: —The epithet refers to the Capibaribe River where the sediment was collected and from where the oldest strain used in this study was isolated. Types: — BRAZIL . Pernambuco state : Recife municipality, sediment from the margins of the Capibaribe river, next to the Hospital Evangélico, 18 July 1997 , Â. Coimbra. Holotype (slide preparation) is deposited in the HURM herbarium (Recife, Brazil): HURM 95350; ex-type strain: URM 3883). Diagnosis: — Aspergillus capibaribensis produces uniquely uniseriate conidiophores with hyaline walls, short phialides 5–6.5 × 3 µm and has vesicles of 12–42 µm. On MEA, MEA 20% sucrose, CZ and CZ 20% sucrose media (14 d, 20 °C), the colony of A. capibaribensis has a cottony texture. On CZ shows growth of 32–34 mm and does not produce exudates. On CZ 20% sucrose shows growth of 21–24 mm . Habitat: —Sediment from the margins of the Capibaribe River in Recife ( Brazil ) and in bee honey (Atlantic Forest, Brazil ). Suborder classification: — Eurotiales , Aspergillaceae . Infrageneric classification: —subgenus Cremei , section Cremei , series Wentiorum . FIGURE 1. A phylogenetic tree based on Maximum-likelihood (ML) analysis was constructed using sequences of ITS- CaM - BenA - rpb 2 of species included in Aspergillus section Cremei . The new species described in this study, Aspergillus capibaribensis , is highlighted in bold. Ex-type strains are marked with an Ex. Values for BS-ML ≥70 % are included near nodes. The tree was rooted to Aspergillus glaucus NRRL 117. Conidiophores uniseriate, stipes hyaline, smooth-walled, septate, 700–1102 (–1219) × 10.5–21 μm. Vesicle globose to subglobose, 12–42 µm. Phialides ampulliform, 5–6.5 × 3 µm. Conidia smooth, globose to rarely slightly ellipsoidal, hyaline, 2–5.5 µm. Hülle cells and sclerotia absent. Sexual morph was not observed. MEA 20% sucrose the fungus sometimes produces dilations in the hyphae, globose to subglobose, intercalary and terminal, 10–21 µm. Colony diameter (7d, 25 º C, in mm): —MEA-bl 25–27; CYA 32–33; CZ 26–28; YES 30–32; OA 17–18; CYAS 41–43; DG-18 34–37. MEA-bl and CYA (7d, 15 ºC, in mm): 5–6; MEA-bl 37 °C no growth; CYA 37 ºC no growth. MEA-bl 42 °C no growth; CYA 42 °C no growth. Colony diameter (14d, 20°C, in mm). – CZ 20% sucrose 21–24; CZ 32–34; MEA 28–30; MEA 20% sucrose 29–30. Culture characteristics (7d, 25 °C, in the dark): —Colonies on MEA-bl, mycelium white, margins entire, centrally convex, radially sulcate, colony texture cottony, sclerotia absent, poor sporulation, conidial colour en masse straw (16), exudate and soluble pigment absent; reverse buff (45) with shades of hazel (88). Colonies on CYA, mycelium white, margins entire with radial sulcate and concentrically, centrally discreetly convex, colony texture cottony, sclerotia absent, poor sporulation, conidial colour en masse indeterminate, exudate and soluble pigment absent; reverse buff (45) at the margins and honey (64) in the centre. Colonies on CZ, mycelium white, margins entire, centrally flat, plain, colony texture cottony, sclerotia absent, sporulation absent, exudate and soluble pigment absent; reverse white. Colonies on YES, mycelium white and aerial, margins entire and regular, centrally convex, discreetly sulcate at margins, colony texture cottony, sclerotia absent, sporulation absent, exudate and soluble pigment absent; reverse pale luteous (11) at margins and at centre luteous (12). Colonies on OA, mycelium white and aerial, margins entire and regular, centrally flat, plain, colony texture floccose, sclerotia absent, sporulation concentrated in the centre, conidial colour en masse pure yellow (14), exudate and soluble pigment absent, reverso pale luteous (11). Colonies on CYAS, mycelium white and aerial, margins entire and regular, convex, radial sulcate, texture cottony, sclerotia absent, sporulation absent, exudate and soluble pigment absent; reverse straw (16). Colonies on DG-18, mycelium white and aerial, margins entire and regular, centrally umbonate, radial sulcate, texture cottony, sclerotia absent, sporulation absent, exudate and soluble pigment absent; reverse pale luteous (11). Colonies on CREA, moderate acid production. FIGURE 2 . Aspergillus capibaribensis . A. colonies, after 7 d at 25 °C in the dark, from left to right (top row) obverse and reverse of MEAbl, CYA, and CYAS; (bottom row) obverse and reverse of DG18, OA and verse of YES and CREA. B-C. Conidiophore and conidial. D. Dilations in the hyphae, after 14 d at 20 °C on MEA 20S. E. Conidia . Photographs by M.T.C. Felipe and R.N. Barbosa. Culture characteristics (14d, 20 °C, in the dark): —Colonies on CZ 20% sucrose, mycelium white and centrally sulphur yellow (15), flat, cottony, margins branching, plain, sclerotia absent, sporulation absent, exudate and soluble pigment absent; reverse inconspicuous. Colonies on CZ, similar to CZ 20% sucrose. Colonies on MEA , mycelium white, margins entire to umbonate, radially sulcate, colony texture cottony, sclerotia absent, poor sporulation, conidial colour en masse straw (16), sclerotia absent, exudate and soluble pigment absent; reverse salmon (41). Colonies on MEA 20% sucrose, mycelium white and aerial, margins entire, crateriform, discreetly radially sulcate, flat, cottony, conidial colour en masse straw (16), sclerotia absent, exudate and soluble pigment absent; reverse salmon (41). Other material examined: — BRAZIL . Pernambuco state : Paulista municipally ( 7°55‘46.9“S 34°53‘12.0“W ), isolated from pollen pots inside nests of Melipona scutellaris , 10 Oct 2019 , R.N. Barbosa & J.E.F. Santos (URM 8768). GenBank numbers: — URM 3883 ex-type, ITS = OR034281, BenA = OR037107, CaM = OR137579, and rpb 2 = OR137581. Notes: — Aspergillus capibaribensis is morphologically and physiologically related to A. flaschentraegeri , some distinctive morphological characteristics were observed. Morphologically, these two species presented some distinctive characteristics such as colony growth and texture on MEA . Aspergillus capibaribensis displayed cotton colonies with growth between 28–30 mm , whereas A. flaschentraegeri formed felt colonies with growth between 20–30 mm . When cultured on MEA 20% sucrose, A. capibaribensis presented cotton colonies and growth of 29–30 mm , while A. flaschentraegeri presented felt colonies and growth of 60–70 mm . On CZ, A. capibaribensis has cotton colonies with growth between 32–34 mm and did not produce exudates, while A. flaschentraegeri had felt colonies with growth up to 30 mm and production of exudates. On CZ 20% sucrose, A. capibaribensis presented cotton colonies and growth of 21–24 mm , while A. flaschentraegeri formed felt colonies and growth of 50 mm . However, A. capibaribensis could be distinguished by its smaller (2–5.5 µm) and smoother conidia, while those of A. flaschentraegeri were larger (4.5–6 × 4–5 μm) and rougher. Conidiophores in A. capibaribensis were exclusively uniseriate, while those in A. flaschentraegeri could be biseriate. The vesicles, although having a similar shape (globose to subglobose) differed in size, with those of A. capibaribensis (12–42 µm) being smaller than those of A. flaschentraegeri (60–140 µm) (Stolk 1964) ( Table 2 ).