Two new Pseudopestalotiopsis species isolated from Celtis sinensis and Indocalamus tessellatus plants in southern China Author Yang, Qi 0000-0001-5420-6838 Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, 550001, China & a 1340156649 @ sina. com; https: // orcid. org / 0000 - 0001 - 5420 - 6838 a1340156649@sina.com Author He, Yu-Ke 0000-0003-3043-0493 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand & School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand & 1033784367 @ qq. com; https: // orcid. org / 0000 - 0003 - 3043 - 0493 1033784367@qq.com Author Yuan, Jun 0000-0002-0529-9852 Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, 550001, China & yuanjun 20190909 @ 163. com; https: // orcid. org / 0000 - 0002 - 0529 - 9852 Author Wang, Yong 0000-0003-3831-2117 Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, 550001, China & yongwangbis @ aliyun. com; https: // orcid. org / 0000 - 0003 - 3831 - 2117 yongwangbis@aliyun.com text Phytotaxa 2022 2022-04-22 543 5 274 282 journal article 55861 10.11646/phytotaxa.543.5.2 61ba3e55-9c41-42d8-bc5b-6ddff18a09d8 1179-3163 6479412 Pseudopestalotiopsis indocalami Qi Yang & Yong Wang bis, sp . nov . ( Fig. 3 ) MycoBank: MB 842469 Index Fungorum: IF 559462. Etymology . indocalami , refers to the host plant ( Indocalamus tessellatus ) from which the fungus was isolated. Type . China , Hainan Province , Wanning City , from leaves of Indocalamus tessellatus , 14 November 2020 , YK He, HGUP 1072 , holotype , ex-type living culture GUCC 21600 . Disease symptom : Associated with leaf spots of Indocalamus tessellatus . Leaf spots 12–68 mm diam., irregular to subcircular, brown, slightly sunken, scattered. Small auburn spots appeared initially and then gradually enlarge, changing to reddish-brown circular ring spots with a dark mahogany border and jagged edge. Description : Asexual morph: Colonies on PDA reaching 6–7 cm in diam. after 7 d at room temperature (28 ˚C), under 12 hours of light-dark alternation. Mycelium light pink to light yellow, colonies filamentous to circular, slightly undulate at edge, whitish, with clustered black fruiting bodies, obviously filiform and fluffy margin, light pink from above and light yellow from reverse. Conidiomata pycnidial, 200–400 μm diam., globose, solitary, black, semiimmersed on PDA, exuding brown to dark brown mass of conidia. Conidiophores often reduced to conidiogenous cell, regularly septate and branched at the base. Conidiogenous cells mostly integrated, ampulliform, cylindrical, or clavate, hyaline, smooth-walled. Conidia fusiform to clavate, straight to slightly curved, 4-septate, 24–31 × 4.5–6.5 ( x = 27.5 × 5.4 µm), basal cell cylindrical to obconic, hyaline, thin-walled, smooth, 2.5–5.5 µm ( x = 3.9 µm) long, three median cells 14–18.5 µm ( x = 16.2 µm) long, concolourous, dark brown with darker septa, second cell from base 4–7 µm ( x = 5.6 µm) long, third cell 3–6 µm ( x = 4.4 µm) long, fourth cell 4–6.5 µm ( x = 5.3 µm) long, apical cell 3.5–6.5 µm ( x = 4.8 µm) long, cylindrical to sub-cylindrical, hyaline, with 2–4 (mostly 3) tubular apical appendages, arising from the apex of the apical cell each at different points, 14–28 µm ( x = 19.7 µm) long, basal appendage usually present, single, tubular, unbranched, 4–6.5 µm ( x = 5.1 µm) long. Sexual morph: undetermined. Notes : Pseudopestalotiopsis indocalami (GUCC 21600) formed an independent branch in the phylogeny ( Fig. 1 ) and was related to P . curvatispora (MFLUCC 17-1722 T , MFLUCC 17-1723, MFLUCC 17-1747). Comparing the three gene regions of GUCC 21600 and P . curvatispora there was only one basepair difference in the ITS region, but five in tub2 and 15 in the tef1 region ( TABLE 2 ). Pseudopestalotiopsis curvatispora has smaller conidia than P . indocalami ((18.5–)22–25(–26.5) × (6–)6.5–7 µm), only 1–2 apical appendages and a longer basal appendage ((5.5–)9–12(–13.5) µm) ( Norphanphoun et al . 2019 ). Thus, P . indocalami is considered to be a novel taxon.