Redescription of Nogueirapis Moure, 1953 with two new species from the Amazon forest (Hymenoptera: Apidae: Meliponini) Author Nogueira, David Silva 0000-0003-2045-0191 Laboratório de Hymenoptera, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia-INPA, Av. André Araújo, 2936 - Petrópolis, CEP 69067 - 375, Manaus, Amazonas, Brazil & dsnogueira @ ifam. edu. br, davidsn @ zootecnista. com. br; https: // orcid. org / 0000 - 0003 - 2045 - 0191 davidsn@zootecnista.com.br Author Ribeiro, Cristiano Feitosa 0000-0002-8831-8800 Laboratório de Hymenoptera, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia-INPA, Av. André Araújo, 2936 - Petrópolis, CEP 69067 - 375, Manaus, Amazonas, Brazil & cristiano. emarc @ gmail. com; https: // orcid. org / 0000 - 0002 - 8831 - 8800 cristiano.emarc@gmail.com Author Oliveira, Marcio Luiz De 0000-0002-3950-1086 Laboratório de Hymenoptera, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia-INPA, Av. André Araújo, 2936 - Petrópolis, CEP 69067 - 375, Manaus, Amazonas, Brazil & mlolivei @ inpa. gov. br; https: // orcid. org / 0000 - 0002 - 3950 - 1086 mlolivei@inpa.gov.br text Zootaxa 2020 2020-10-05 4859 1 138 150 journal article 8239 10.11646/zootaxa.4859.1.6 a57d7bd0-ee60-4db8-bda6-7db21e3e46fb 1175-5326 4412862 73669ABE-C1AA-44EF-8B70-EB7C6622C764 Nogueirapis rosariae sp. nov. Nogueira Figures 05, 14, 15, 20, 25 and 26E Diagnosis (worker): Mesoscutum pale (brown to black in N. costaricana , N. minor , N. mirandula and N. batistai sp. nov. ) coloration predominant yellow to yellowish-brown with yellow spots on frons and mesonotum, poorly contrasted. Anterior third of mesoscutum yellowish-brown. Apical third of metatibia with posterior half yellowishbrown. Paraocular spot not completely surrounding the antennal socket. N. rosariae sp. nov. is apparently very close to N. butteli , due the smooth and shiny integument of head, but the new species differs in the shorter body length, narrower metatibia, paraocular spot not surrounding the antennal socket, and light brown coloration in the anterior third of the mesoscutum (totally yellowish in some specimens) in relation to the dark brown of N. butelli , besides having the apical third of the terga yellowish to reddish brown. Coloration: integument predominantly yellow to yellowish-brown. Paraocular area with the yellow spot more than 2x the diameter of the flagellum, reaching the antennal socket and the upper edge of the compound eye. Clypeus, supraclypeal area, malar area, labrum, radicle and gena yellow. Mandible yellow with apical margin reddish, condyles yellow. Scape yellow with apical half of posterior surface yellowish-brown. Pedicel dark brown. Pronotal lobe yellow. Mesoscutum yellowish-brown with yellow lateral lines. Axilla yellow. Posterior margin of scutellum yellow. Propodeum, mesepisternum and metepisternum yellow. Proleg yellow with protarsi slightly darker than the rest of the leg. Mesoleg, metacoxa, metatrochanter and metafemur yellow. Metatibia yellow with brown spot on posterior half of apical third. Metabasitarsus dark brown with yellowish-brown margins. Metatarsi yellow slightly darker than on metacoxa. Tegula yellowish-brown. Pterostigma and wing venation dark brown. T1 yellow with apical third dark brown. T2 yellowish-brown with basal third dark-brown. T3–T5 yellowish-brown. T6 and S1–S5 yellow. Punctation: predominantly smooth and shiny; propodeum with lateral side rugose. FIGURES 6–15. Body of species of Nogueirapis Moure, 1953 in lateral and dorsal view respectively for each species. 6, 7: Nogueirapis minor ( Moure & Camargo, 1982 ) . 8, 9: Nogueirapis mirandula ( Cockerell, 1917 ) . 10, 11: Nogueirapis butteli ( Friese, 1900 ) . 12, 13: Nogueirapis batistai sp. nov. 14, 15: Nogueirapis rosariae sp. nov. Scale bar: 1 mm. Pubescence: frons only with simple hairs ( 0.04 mm ); some hairs behind genal area with curved apex ( 0.16 mm ); lateral area of antennal socket with some hairs with two branches; simple hairs on labrum ( 0.15 mm ) and simple setae on vertex ( 0.13 mm ); simple hairs on coxae ( 0.16 mm : 0.2 mm : 0.21 mm = procoxa, mesocoxa and metacoxa, respectively); some hairs with curved apex on metacoxa; some hairs on trochanters with curved apex ( 0.13 mm : 0.19 mm : 0.14 mm = protrochanter, mesotrochanter and metatrochanter, respectively); femora with yellow and simple hairs on basal ventral face and with yellowish-brown short setae on apical dorsal surface (0.13/ 0.03 mm : 0.16/ 0.06 mm : 0.11/ 0.1 mm = profemur, mesofemur and metafemur, respectively); metatibia with corbicular hair ( 0.58 mm ), almost as long as the length of scape (there are two other long hairs on corbicula: 0.53 mm and 0.32 mm , the last hair is closest to the posterior margin); posterior margin of metatibia with yellowish setae ( 0.22 mm ); hairs of ventral surface of metatibia uniformly distributed posteriorly, leaving just a very narrow margin glabrous; basitarsi with yellowish and simple setae ( 0.23 mm : 0.1 mm : 0.19 mm = probasitarsus, mesobasitarsus and metabasitarsus, respectively); mesoscutum with erect setae ( 0.07 mm ) 2.3x length of decumbent setae ( 0.03 mm ); propodeal triangle glabrous; lateral sides of propodeum with long and simple hairs ( 0.13 mm , some with curved apex), short simple ( 0.05 mm ) and branched hairs ( 0.05 mm ); hair with more branches around the propodeal spiracle ( 0.09 mm ); lateral area of mesepisternum with erect hairs ( 0.09 mm ) and decumbent hairs ( 0.04 mm ); inferior half of mesepisternum with long simple hairs ( 0.25 mm ); metepisternum with decumbent and simple hairs or with one branch ( 0.04 mm ); scutellum with simple hairs ( 0.21 mm ) on dorsal surface and simple and branched setae ( 0.12 mm ) on posterior surface; pronotal lobes with long and simple hairs ( 0.12 mm ) and short branched hairs ( 0.06 mm ); T1 glabrous; T2 almost completely glabrous, with more visible setae on lateral sides ( 0.04 mm ); simple setae on T3 ( 0.06 mm ), T4 ( 0.09 mm ), T5 ( 0.08 mm ) and T6 ( 0.09 mm : 0.15 mm = disc and lateral area, respectively); Sterna with simple hairs ( 0.23 mm : 0.21 mm : 0.16 mm : 0.14 mm : 0.09 mm = length of S1, S2, S3, S4 and S5, respectively). FIGURES 16–20. External surface of metatibia and metabasitarsus of species of Nogueirapis Moure, 1953 . 16: Nogueirapis minor ( Moure & Camargo, 1982 ) . 17: Nogueirapis mirandula ( Cockerell, 1917 ) . 18: Nogueirapis butteli ( Friese, 1900 ) . 19: Nogueirapis batistai sp. nov. 20: Nogueirapis rosariae sp. nov. Scale bar: 0.5 mm. Structures: length of compound eye 3.67x its width; inner orbits of compound eyes concave, slightly converging downward; interorbital distance shorter than length of compound eye ( 1.14 mm : 0.95 mm : 1.13 mm : 0.89 mm = length of compound eye and upper, median and lower interorbital distances respectively); malar area linear ( 0.05 mm ), its length less than half width of the flagellum; clypeus length slightly less than half its width ( 0.38 mm : 0.83 mm = clypeal length and width, respectively), clypeus gently convex in lateral view; interalveolar distance slightly larger than diameter of antennal sockets, and much shorter than alveolorbital distance ( 0.19 mm : 0.24 mm : 0.15 mm = interalveolar and alveolorbital distance, and diameter of antennal socket respectively); frons slightly depressed along median line; vertex slightly convex, without postocellar carina; interocellar distance equals to the ocellorbital distance ( 0.19 mm ); posterior margin of head slightly concave, without preoccipital carina; scape length 5,4x its width ( 0.54 mm : 0.1 mm ); pedicel slightly larger than the ninth flagellomer ( 0.12 mm : 0.11 mm ); anterior margin of pronotum slightly concave; scutellum paraboloid slightly projected backward; length of propodeal spiracle 4.6x its width (measured from the inner margin); metatibia subtriangular with posterior distal margin slightly angulated; length of hind tibia 2.5x its width ( 1.3 mm : 0.52 mm ); metabasitarsus 2x longer than broad ( 0.67 mm : 0.33 mm ) with posterior angle projected downward, overcoming the encounter line with the following tarsus; vein separating first and second submarginal cells (Rs) and separating second and third (2 nd rs-m) virtually absent. Other measures are available in table 1. FIGURES 21–25. Integumental surface of the head of species of Nogueirapis Moure, 1953 . 21: Nogueirapis minor ( Moure & Camargo, 1982 ) . 22: Nogueirapis mirandula ( Cockerell, 1917 ) . 23: Nogueirapis butteli ( Friese, 1900 ) . 24: Nogueirapis batistai sp. nov. 25: Nogueirapis rosariae sp. nov. Scale bar: 0.05 mm. Queen: Unknown. Male: Unknown. Etymology: The epithet is homage to Maria do Rosario Silva, mother of the first author. Examined material: Holotype : Worker ( INPA ): BRASIL . Amazonas , ENSENA Juami-Japurá , Médio Rio Juami , 01°57’20.4′′S , 67°55’47.8′′W / 23–29 xix 2004, M.L. Oliveira & F.F. Xavier Filho leg., Em roupa suada. Paratypes ( 37 workers ): BRAZIL , Amazonas : 2 ( INPA ): Brasil—AM, C. Castanho , BR 319 , Km 181, Sítio São Paulo / 04°12’57′′S– 60°49’13′′W , 14.vii.2016 , M. L. Oliveira leg. 2 ( INPA ): BRASIL, Amazonas , ′′ Campo do Tupana′ ′, ca. 120 km S. Manaus / Mata— 04.15679 S , 60.13203 W , 03/VII/2007 , geral, Expedição GEOMA , H. F. Guariento leg. 3 ( INPA ): BRASIL. Amazonas , ENSENA Juami-Japurá , Médio Rio Juami , 01°57’20.4′′S , 67°55’47.8′′W / 23–29 xix 2004, M.L. Oliveira & F.F. Xavier Filho leg., Em roupa suada. 1 ( RPSP ): idem. 1 ( MPEG ): idem. 1 ( MZSP ): idem. 1 ( INPA ): Brasil : Amazonas , Estirão del Ecuador , 28-X-1976 , Bob Hill. 26 ( INPA ): Brasil-Amazonas , Rio Purus. Beruri , 09/X/2002 / Xavier Filho, F.F & Barbosa, U.C. , Em Malaise . FIGURE 26. Dorsal view of mesonotum of species of Nogueirapis Moure, 1953 , with detail to escutum-escutelar suture between axillae. A: Nogueirapis minor ( Moure & Camargo, 1982 ) . B: Nogueirapis mirandula ( Cockerell, 1917 ) . C: Nogueirapis butteli ( Friese, 1900 ) . D: Nogueirapis batistai sp. nov. E: Nogueirapis rosariae sp. nov. . TABLE 1. Measurements (mm) of workers of Nogueirapis Moure, 1953 species: N. butteli ( Friese, 1900 ) , N. minor Moure & Camargo, 1982 , N. mirandula ( Cockerell, 1917 ) , N. batistai sp. nov. (holotype from, São Gabriel da Cachoeira, Amazonas state, Brazil), N. rosariae sp. nov. (holotype from middle Juami River, Amazonas state, Brazil). One worker of each species was measured.*unreliable because the compound eye is withered.