Molecular characterization of Helicotylenchus multicinctus and H. dihystera (Tylenchida: Hoplolaimidae) from Theobroma cacao in Nigeria Author Daramola, Fisayo Y. Author Orisajo, Samuel B. Cocoa Research Institute of Nigeria, PMB 5244, Ibadan, Nigeria Author Malan, Antoinette P. apm @ sun. ac. za; https: // orcid. org / 0000 - 0002 - 9257 - 0312 Author Marais, Mariette Nematology Unit, Biosystematics, ARC, Plant Health and Protection, Private Bag X 134, Queenswood 0121, South Africa MaraisM @ arc. agric. za; https: // orcid. org / 0000 - 0002 - 6681 - 9903 text Zootaxa 2020 2020-05-14 4778 2 343 356 journal article 22154 10.11646/zootaxa.4778.2.6 c06d4540-af0b-46a1-9a1a-05176fae3ab4 1175-5326 3826378 084DEF45-393D-4C81-A0C7-CD83A9529F8C Helicotylenchus multicinctus ( Cobb, 1893 ) Golden, 1956 = Tylenchus multicinctus Cobb, 1893 = Tylenchorhynchus multicinctus ( Cobb, 1893 ) Micoletzky, 1922 = Anguillulina multicincta ( Cobb, 1893 ) Goodey, 1932 = Rotylenchus multicinctus ( Cobb, 1893 ) Filipjev, 1936 = Rotylenchus iperoiguensis Carvalho, 1956 = Helicotylenchus iperoiguensis ( Carvalho, 1956 ) Andrássy, 1958 Description. Habitus spiral. Lip region hemispherical, slightly offset, with three to five annuli. Labial framework heavily sclerotized. Outer margins conspicuous and extending posteriorly for 3 to 4 body annules. Cephalids not seen. Stylet well developed (21–24) µm. Stylets knobs prominent ( 5–6 µm ), wide, appearing anteriorly flattened or concave. Position of DGO about 1/4 the total stylet length and posteriorly located behind the stylet knobs. Median bulb round to oval, with a small, centrally placed valve. Glandular part of pharynx overlapping the intestine ventrally. Excretory pore close to pharyngo-intestinal junction. Epiptygma folded into vagina. Hemizonid two to three annules long, located 0–3 annules anterior to excretory pore. Ovaries paired and symmetrical, posterior branch slightly reduced than the anterior. Spermathecae slightly offset, rounded and filled with sperms. Vulva a depressed transverse slit at 67% of the body length. Phasmids pore-like and located from one to six annules anterior to anus level. Tail slightly tapering and curved more dorsally than ventrally, with six to fifteen annules. Ventral caudal projection and mucro absent. Remarks. The morphological and morphometric characters of this species are consistent with those previously reported in the original description of H . multicinctus from Nigeria ( Sher 1966 , Siddiqi 1972 , Ali et al. 1973 ).