Description of New Species of Coronostrongylus and Dorcopsistrongylus (Strongylida: Chabertiidae) from Dorcopsis muelleri (Macropodidae) from Kumawa Mountains, West Papua, Indonesia Author Purwaningsih, Endang Author Dewi, Kartika Author Smales, Lesley R. text Zootaxa 2019 2019-03-11 4565 3 433 441 journal article 28389 10.11646/zootaxa.4565.3.10 2656041e-a0d6-498b-b1b7-718810fe89b4 1175-5326 2590246 3B766431-5C26-40DD-91D2-931912CFF463 Dorcopsistrongylus supriyatnai n. sp. ( Figs. 16–28 ) Description. General: Body small, attenuated posteriorly, cuticle with fine transverse striations. Anterior end with cephalic collar, without fine longitudinal striae, bearing 4 submedian papillae, each with single seta, between elements of the labial crown ( Fig. 27 ). Mouth opening circular, labial crown divided into 4 lip-like elements, anterior end of each ridged, amphids, 1 each on base of lateral lip ( Fig. 26 ). Lateral labial crown elements larger than dorsal and ventral elements. Buccal capsule cylindrical, thick walled, lining with prominent transverse striations ( Fig. 17 ), longer than deep. Anterior end of buccal capsule with labial collar, lacking fine longitudinal striae. Oesophagus about 15.6% body length, lining strongly sclerotized, cylindrical with elongated terminal bulb following slight constriction ( Fig. 16 ). Nerve ring at anterior extremity, deirids setate ( Figs. 16 , 28 ), posterior to nerve ring, excretory pore posterior to deirids. Anterior end of intestine without diverticula. Male (n=12): Body length 9.50 (8.70–12.30) mm; width 473 (365–530). Buccal capsule 58 (50–70) long, 19 (15–23) wide. Oesophagus 1,485 (1,370–1,710) long, bulb 217(180–240) long. Nerve ring 338 (230–400), deirids 396 (320–670), excretory pore 487 (410–580) from anterior end, respectively. Bursal lobes not separate, dorsal lobe longest, without median cleft, ventral lobes shortest. Ventro-ventral and ventro-lateral rays apposed, reaching bursal margin, externo-lateral ray divergent, shorter than medio-lateral and postero-lateral, not reaching bursal margin, medio-lateral and postero-lateral apposed, reaching bursal margin, externo-dorsal ray arising from the lateral trunk, not reaching margin of bursa; dorsal trunk stout, bifurcating at about 1/4 its length, each branch giving off pair of short lateral branches at 2/3 its length ( Fig. 22 ). Spicule slender, wider at proximal end ( Fig.18 ), pointed at distal end ( Fig.19 ), 1,942 (1,860–2,090) long, about 20.3% body length. Gubernaculum bean shaped, 17 long, 34 wide ( Fig. 21 ). Genital cone prominent, dorsal lip conical, ventral lip with 1 pair of bifid appendages and 2 pair simple l appendages ( Fig. 20 ). Female (n=18): Body length 13.60 (8.25–19.00) mm; width 576 (400–680); buccal capsule 71 (60–78) long 38 (25–40) wide. Oesophagus 1,711 (1,180–1,950) long. Nerve ring 409 (330–580), deirids 496 (370–570), excretory pore 577 (410–630) from anterior end, respectively. Vulva 727 (440–749) from tip of tail. Ovejector with long and sinuous shaped, vagina vera 973 (850–1190) long, vestibule large, 283(260–305), sphincter short, 76 (50–98) and infundibulum not seen ( Fig. 23 ). Tail tapering, with pointed tip, 349 (320–420) long ( Fig. 24 ). Eggs thin-shelled, oval, 71(60–78) long, 38 (35–40) wide ( Fig. 25 ). Type host: Dorcopsis muelleri (Lesson) (Mammalia: Macropodidae ). Type locality: Kumawa Mountains , West Papua , Indonesia (1°6 S, 130°51 E). Site of infection: Stomach. FIGURES 16–25. Dorcopsistrongylus supriyatnai n. sp. 16. Anterior end, ventral view. 17. Buccal capsule, lateral view. 18. Spicule, proximal end, ventral view. 19. Spicule, distal end, lateral view. 20. Genital cone, dorsal view. 21. Gubernaculum, ventral view. 22. Copulatory bursa, dorsal view. 23. Ovejector. 24. Posterior end, lateral view. 25. Egg. Scale bar: 16, 24, 10 0 µm; 17, 18, 19, 20, 21, 22, 23, 25 µm Type specimens: Holotype male, allotype female (MZB Na764); paratypes , 11 males and 17 females (MZBNa 765). Date of collection: November 2014 . Etymology. The name of the species is given in honour of the collector of the host specimens. Remarks. These specimens belong in the genus Dorcopsistrongylus because they have a suite of characters including an external labial crown of four lip-like elements, a long cylindrical buccal capsule lined with prominent transverse striations and a long narrow oesophageal corpus with a clavate terminal bulb ( Smales, 1982 ). Dorcopsistrongylus supriyatnai n. sp. differs from all congeners in lacking large anteriorly directed intestinal diverticulae. The species is most similar to D. labiacarinatus in morphometrics ( Table 1 ), but further differs in having larger lateral than ventral and dorsal labial crown elements, not having fine longitudinal striae on the anterior end of the buccal capsule, a narrower buccal capsule, a longer vagina vera, the proportions of the ovejector, a shorter vestibule and longer sphincter, and a larger, bean shaped compared with a smaller rectangular gubernaculum. TABLE 1. Comparative measurements (µm) of Dorcopsistrongylus spp. (Data from Smales, 1982, Purwaningsih & Smales, 2010 and this study)

MALE	D. labiacarinatus	D. ewini	D. salawatiensis	D. supriyatnai n. sp.
Body length (BL) mm	9.4 (6.0–10.0)	5.57 (4.39–6.47)	7.19 (6.57–8.67)	9.53 (8.70–12.3)
Width	400 (340–450)	402 (330–435)	535 (510–560)	473 (365–530)
Buccal. Capsule: Length width	57 (47–65) 27 (26–29)	83 (65–90) 33 (30–40)	74 (74–90) 23 (23–30)	58 (50–70) 19 (15–23)
Anterior end to Nerve. ring Deirid Excretory pore	390 (340–430) 520 (430–590) 634 (510–690)	358 (320–400) 455 (430–460) 525 (480–650)	359 (320–390) 498 (490–510) 588 (540–630)	338 (230–400) 396 (320–670) 487 (410–580)
Oesophagus length = %BL	1,430 (1,290– 1,600)=15.2%	1,231 (1,250– 1,480)=22,1%	1,336 (1,130– 1,412)=18,6%	1,485 (1,370– 1,710)=15.6%
Spicule length = % BL	1,860 (1,720–2,000) =20,2%	1,709 (850–1,412) =30,7%	1,878 (1,730–1,970) = 26,1%	1,942 (1,860–2,090) =20,3%
Gubernaculum	22.5 long 30 wide	25 long 40 wide	70 long 62 wide	17 long, 34 wide
FEMALE Body length mm Width	11,000 500	7.61 (6.35–9.07) 472 (300–565)	9.20 (8.02–10.55) 639 (570–720)	13.60 (8.30–19.00) 576 (400–680)
Buccal Capsule: Length Width	68 36	103 (100–110) 40 (35–50)	84 (62–96) 29 (26-31)	71 (60–78) 38 (25–40)
Anterior end to Nerve ring Deirid Excretory pore	410 490 630	374 (350–420) 555 (520–590) 653 (575–700)	409 (360–440) 542 (510–580) 682 (600–770)	409 (330–580) 496 (370–570) 577 (410–630)
Oesophagus length Tail Vulva–post end Egg	1530 350 640 76 (68–80) x 40 (34.5–44)	1,550 (1,420–1,620) 404 (330–480) 640 (520–710) 71 (63–79) x 38 (32–43)	1,688 (1,580–1,800) 393 (360–400) 592 (436–700) 62 (58–69) x 32 (28–38)	1,711 (1,180–1,950) 349 (320–420) 727 (440–749) 71 (60–78) x 38 (35–40)
Vagina vera Vestibule Sphincter Infundibulum	630 - 200 200	446 (442–449) 323 (317–328) 169 (156–182) 64 (60–68)	715 (699–736) 367 (338–395) 209 (200–218) 93 (86–99)	973 (850–1190) 283 (260–305) 76 (50–98) -

FIGURES 26–28. Scanning electron micrographs of Dorcopsistrongylus supriyatnai n.sp. 26. Cephalic end, apical view. 27. Setate cephalic papillae. 28. Deirid with seta, lateral view. Scale bar: 26, 10 µm; 27, 28, 2 µm Dorcopsistrongylus supriyatnai further differs from D. ewini in being a larger worm with a shorter buccal capsule, proportionally shorter oesophagus, shorter spicules without the proximal twist, larger gubernaculum, longer vagina vera and an ovejector with a shorter sphincter ( Table 1 ). Dorcopsistrongylus supriyatnai further differs from D. salawatiensis in being a larger worm with a narrower buccal capsule, proportionally shorter oesophagus and spicules, larger gubernaculum, longer vagina vera, and an ovejector with a smaller vestibule and sphincters ( Table 1 ). Dorcopsistrongylus supriyatnai , D. ewini and D. salawatiensis are all found in Do. muelleri from different localities in Papua, while D. labiacarinatus is found in Do. luctuosa , from Papua New Guinea .