Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes) Author Hou, Lingwei Author Hernandez-Restrepo, Margarita Author Groenewald, Johannes Zacharias Author Cai, Lei Author Crous, Pedro W. text MycoKeys 2020 65 49 99 http://dx.doi.org/10.3897/mycokeys.65.47704 journal article http://dx.doi.org/10.3897/mycokeys.65.47704 1314-4049-65-49 DED2BF86E6FD56179642F7119604CEB9 Paraboeremia truiniorum Hern.-Restr., L. W. Hou, L. Cai & Crous sp. nov. Figure 8 Etymology. truiniorum refers to Cuno & Tygo Truin who collected the soil sample from which the ex-type strain was isolated. Typus. The Netherlands. Gelderland province, Barneveld, Voorthuizen, isolated from garden soil, Mar. 2017, C. Truin & T. Truin ( holotype designated here CBS H-24108, living ex-type culture CBS 144952 = JW 47002). Conidiomata pycnidial, superficial, scattered or aggregated, most solitary, globose or subglobose, confluent and irregularly-shaped with age, pale brown, thick-walled, covered with abundant mycelial outgrowths, 160-420 x 135-430 μm ; 1-papillate or non-papillate ostioles, sometimes elongated to a short neck; pycnidial wall pseudoparenchymatous, 7-11 layers, 40-70 μm thick, outer layers composed of brown, flattened polygonal cells of 22-45.5 μm diam. Conidiogenous cells phialidic, hyaline, smooth, globose, subglobose, ampulliform or doliiform, 4.5-8.5 x 4-7 μm . Conidia ellipsoidal to oblong, thin- and smooth-walled, hyaline, aseptate, 3.5-5 x 2-3 μm , with (1-)2 large guttules. Conidial matrix whitish. Culture characteristics. Colonies after 7 d at 25 °C, on OA reaching 70-75 mm diam, aerial mycelium floccose, vinaceous buff to hazel, margin regular; reverse buff to olivaceous. On MEA reaching 65-70 mm diam, aerial mycelium felty, whitish, pale mouse grey toward periphery, margin regular; reverse dark brick to dark brown, with pale brown edge. On PDA reaching 75-80 mm diam, aerial mycelium felty, olivaceous buff to pale mouse grey, olivaceous toward periphery, margin irregular; reverse mouse grey, olivaceous toward periphery. NaOH spot test negative on OA. Additional specimens examined. The Netherlands, Gelderland province, Culemborg, isolated from garden soil, Mar. 2017, R. Fuld, JW 182014; The Netherlands. South Holland province, Alphen aan den Rijn, isolated from garden soil, Mar. 2017, K. Boutwell, CBS 144961 = JW 203021; The Netherlands. South Holland province, Gorinchem, isolated from garden soil, Mar. 2017, L. van Rosmalen, JW 270002; The Netherlands. Utrecht province, Utrecht, isolated from garden soil, Mar. 2017, L. van Rijnberk, JW 147025; The Netherlands. Utrecht province, Woerden, isolated from garden soil, Mar. 2017, L. Borsboom, JW 192003. Notes. Based on the phylogenetic analyses, P. truiniorum is represented by six isolates, forming a distinct lineage (Figure 1 ). Paraboeremia truiniorum is characterised by the dense mycelial outgrowths on its pycnidia. Both P. truiniorum and P. rekkeri are phylogenetically close to the well-known soil-borne species, P. putaminum . However, P. putaminum is distinguished from these two new species by producing smaller conidia (3.2-4.2 x 2-2.6 μm ) with greenish guttules ( Boerema et al 2004 ). Figure 8. Paraboeremia truiniorum (CBS 144952). A, B Colony on OA (front and reverse) C, D colony on MEA (front and reverse) E, F colony on PDA (front and reverse) G pycnidia forming on OA H pycnidium I section of pycnidium J section of pycnidial wall K-N conidiogenous cells O conidia. Scale bars: 20 μm ( H ); 50 μm ( I ); 5 μm ( J-O ).