A redescription of the chigger Hannemania achalai Alzuet and Mauri, 1987 (Acariformes: Prostigmata: Leeuwenhoekiidae) in frogs from Sierra Grande, Cordoba, Argentina Author Paredes-León, Ricardo Colección Nacional de Ácaros, Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México. Tercer circuito exterior s / n, 04510, Ciudad de México, México. Author Biolé, Fernanda G. Instituto de Investigaciones en Producción Animal (INPA-CONICET-UBA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín, 280, C 1427 CWO, Buenos Aires, Argentina. Author Valetti, Julián A. Ecología, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional N ° 36 - km. 601, X 5804 BYA Río Cuarto, Argentina. Author Martino, Adolfo L. Ecología, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional N ° 36 - km. 601, X 5804 BYA Río Cuarto, Argentina. text Acarologia 2018 2018-01-31 1987 1 159 164 journal article 8470 10.24349/acarologia/20184234 3925bd74-90ec-4d2a-92b5-ec3dc6234947 2107-7207 4487485 2B6FE32E-0057-41C1-A53B-232357AA41F9 Hannemania achalai Alzuet and Mauri, 1987 ( Figures 1–3 ) H. achalai Alzuet and Mauri, 1987: 114 Diagnosis — SIF = 5B–B–3–2111.0000; fPp = B/B/BBB. Pc = 3; Gn = 2; fSc = PL> AL ≥ AM; PL/SB; fCx = 2.1.1; fSt = 0.1; DS = 53 – 75; VS = 49 – 69; NDV = 132; Ip = 765 – 865; AW = 50 – 60; PW = 65 – 80; SB = 20 – 30; ASB = 45 – 55; PSB = 20 – 25; SD = 75 – 80; AP = 15 – 20; AM = 30 – 40; AL = 30 – 40; PL = 60 – 70; S = 75 – 115; H = 40 – 45; D min = 30 – 45; D max = 50 – 65; Vmin = 30 – 35; V max = 35 – 45; pa = 280 – 320; pm = 240 – 275; pp = 250 – 290. Emended description (larva, n = 63) Idiosoma — 950 – 1215 long and 450 – 600 wide ( Figures 1 A–B). Eyes 2 + 2, total number of idiosomal setae range from 102 to 144. Scutum with naso, 70 – 80 long and 70 – 85 wide ( Figure 1E ); diameter of sensillary bases 7 – 9, AMW = 9 – 11. Ventrally with only one pair of posterior sternal setae ( Figure 1B ). Gnathosoma — 95 – 110 long and 60 – 70 wide ( Figures 1 C–D, 3A). Palp, five-segmented with following setal distribution: trochanter without setae, femur and genua with one branched 2009, coll. A. L. Martino, P. R. Grenat and M. Otero. 5 larvae (CNAC007188–007191) ex Pleurodema cordobae [884], ARGENTINA , Córdoba , Mal Paso, Pampa de Achala, - 31°49’52”, -64°51’40”, 2308 m a.s.l., 16 Feb. 2010 , coll. A. L. Martino, J. A. Valetti, P. R. Grenat, M. Otero and F. G. Biolé. 11 larvae (CNAC007192–007197) same data except host [885]. 5 larvae (CNAC007198–007201) ex Pleurodema cordobae [898], ARGENTINA , Córdoba , Cerro Negro, -31°57’26.2”, -64°54’59.7”, 2323 m a.s.l., 5 Nov. 2011 , coll. M. Otero. 12 larvae (CNAC007202–007206) ex Pleurodema cordobae [905], ARGENTINA , Córdoba , Los Tabaquillos, -32°23’58.4”, -64°55’35.1”, 2113 m a.s.l., 22 Nov. 2011 , coll. J. A. Valetti, P. R. Grenat, M. Otero and A. L. Martino. 6 larvae (CNAC007207–007210) ex Pleurodema kriegi [759] ARGENTINA , Córdoba , La Posta, -31°36’43.7”, -64°52’26.7”, 2159 m a.s.l., 4 Dec. 2008, coll. J. A. Valetti, J. Marquez and M. Ammann. 6 larvae (CNAC007211–007213) ex Pleurodema kriegi [862], ARGENTINA , Córdoba , Pampa de Achala, La Trinidad, -31°44’13”, -64°50’58”, 2318 m a.s.l., 5 Jan. 2010 , coll. J. A. Valetti, A. L. Martino and C. Casale. 12 larvae (CNAC007214–007217) ex Pleurodema kriegi [1633], ARGENTINA , Córdoba , Pampa de Achala, El Cóndor, -31°36’23”, -64°52’06”, 2178 m a.s.l., 16 Dec. 1981 , coll. R. Martori. Figure 1 Hannemania achalai Alzuet and Mauri, 1987 (larva): A – dorsal aspect of idiosoma, B – ventral aspect of idiosoma, C – ventral aspect of gnathosoma, D – dorsal aspect of gnathosoma, E – prodorsum showing scutum and eyes, F – palpal tarsus, G – palpal claw, H – lateral aspect of tarsus I.

Figure 2 Hannemania achalai Alzuet and Mauri, 1987 (larva): A – leg I, B – leg II, C – leg III. All the specialized setae and some representative non-specialized branched setae are illustrated. Scale bar 50 μm.
seta, tibia with dorsal, lateral and ventral setae branched, and tarsus with five branched setae and basal tarsala (Figures 1F, 3B); palpal claw trifurcate (Figures 1G, 3B); galeala branched; cheliceral blade expanded distally with a series of teeth (Figure 3C). Gnathobasal setae branched. Legs — All six-segmented, with pair of claws and claw-like empodium, and with short thin onychotriches. Leg I: coxa with 2 non-specialized branched setae (2B); trochanter 1B; femur 6B; genu 4B, 2 genualae, microgenuala; tibia 8B, 2 tibialae, microtibiala; tarsus 24B, tarsala (21 – 24 long), microtarsala distal of tarsala, subterminala, parasubterminala, pretarsala (Figures 1H, 2A). Leg II: coxa 1B; trochanter 1B; femur 5B; genu 4B, 1 genuala, microgenuala; tibia 6B, 2 tibialae; tarsus 18B, tarsala (20 – 22 long), microtarsala slightly distal of tarsala, pretarsala (Figure 2B). Leg III: coxa 1B; trochanter 1B; femur 4B; genu 4B, 1 genuala; tibia 6B, 1 tibiala; tarsus 16B (Figure 2C).
Hosts — Pleurodema sp., Odontophrynus sp. and O. occidentalis ( Alzuet and Mauri 1987 ) , P. kriegi ( Alzuet and Mauri 1987 ; Biolé et al . 2015 ), and P. cordobae ( Biolé et al . 2015 ) .
Material examined — 6 larvae (CNAC007184–007187) ex Pleurodema cordobae [847], ARGENTINA, Córdoba, Los Linderos, -32°01’04.9”, -64°56’22.9”, 2621 m a.s.l., 15 Dec.

Figure 3 Hannemania achalai Alzuet and Mauri, 1987 (larva): A – gnathosoma, B – palpal tibia and tarsus, C – cheliceral blade. Scale bar 50 μm (A), and 25 μm (B and C). Remarks — All the specimens with 2 genualae I, except one specimen (CNAC007207) with 2 genualae on right leg and 1 genuala on left leg, and one specimen (CNAC007186) with 2 genualae on right leg and 3 genualae on left leg. All the specimens with 1 genuala III, except one specimen (CNAC007184) with 1 genuala on left leg and 2 genualae on right leg. We find that our specimens are conspecific with H. achalai based on the palpal setal formula, the palpal claw, and the number of specialized nude setae on legs: genualae I–III, tibialae I–III, tarsalae and pretarsalae I–II, and subterminala I. However, the specimens analysed in this study differ from the original description by Alzuet and Mauri (1987) in the following characters: 1) the shape of galeala which is sparsely branched instead nude as mentioned by Alzuet and Mauri (1987) , and 2) the presence of 2 genualae I constantly, whereas in the type specimens the number of genualae I range 2–4 ( Alzuet and Mauri 1987 ). These differences are considered here as intraspecific variation until type material can be examined. According to Alzuet and Mauri (1987) and Salazar Martínez et al . (2004) the type material is deposited at Museo de La Plata (MLP), Argentina , however, types were not seen for this study.