Newly recorded chlorophytes, Monoraphidium subclavatum, Deuterostichococcus epilithicus, and Pseudostichococcus monallantoides in Korea Author Yoon, Sung Ro Author Lee, Do Hyun Kim and Ok-Min omlee@kgu.ac.kr text Journal of Species Research 2020 9 3 181 190 journal article 10.12651/JSR.2020.9.3.181 2713-8615 13140693 Monoraphidium subclavatum Nygaard 1977 ( Fig. 1 ) Cells are solitary, spindle-shaped, slightly or moderate crescent-shaped, rarely sigmoid, with both ends gradually tapering to a point, but not in long tips. Chloroplast covers almost the entire cell wall, and is not clearly confined to the center, without a pyrenoid. Propagation is mostly achieved by four autospores, which are slightly crescent-shaped in young cells, but increasingly crescent eventually. The cell length is 13.7 - 22.2 μm and width is 2.4 - 5.3 μm. Note. Spindle-shaped and slightly crescent-shaped cells were mainly observed. The ends of the cells are pointed, but the ends are shorter and the blunt ends differ from those of M. pusuillum (Printz) Komárková-Legnorová. Individuals measuring 14 - 16 μm in length appeared frequently. Phylogenetic analyses using 18S rDNA sequences showed that the genus Monoraphidium is polyphyletic, and Monoraphidium subclavatum formed the same clade with Messastrum gracile . However, the two species are distinguished by cell curvature and colony formation. Fig. 2. Microscopic photographs of Deuterostichococcus epilithicus Pröschold and Darienko. NIBRCL 0000114567. (A - F) slightly curved cylindrical-shapes, (G, J) colony forming circle, (H, I) weakly connected filaments. Scale bar represents 10 μm. Ecology. This is a freshwater species. They inhabit small water biotopes ( Komárek and Fott, 1983 ). This species was collected from planktonic samples of a eutrophic river. Site of collection. Joochun river , Hoengseong-gun , Gangwon-do ( 37°24′44.4″N 128°09′20.0″E ) . Date of collection. May 28, 2019 . Specimen Locality. FBCC-A 409 in the Nakdonggang National Institute of Biological Resources.