Four new species of Saetherocladius Andersen et Mendes from Mata Atlântica, Brazil (Diptera: Chironomidae: Orthocladiinae) Author Andersen, Trond Author Mendes, Humberto F. Author Pinho, Luiz C. text Zootaxa 2010 2608 45 56 journal article 10.5281/zenodo.197747 2f6b9954-71bf-427f-9804-0c228af11d58 1175-5326 197747 Saetherocladius triangulatus sp. n. ( Figs 14–19 ) Type material. Holotype male, slide mounted in Canada balsam. Brazil : São Paulo State, Parque Estadual Intervales, Ribeirão Grande, Barra Grande, 10–13.xii.2000 , Malaise trap (Trilha 4), M.T. Tavares et al. ( MZUSP ). Paratypes 1 male , São Paulo State, Estação Biológica Boracéia, Salesópolis, Trilha dos Pilões, 23o39'04.8''S , 5o53'41.8''W , 30.iii.2001 , Malaise trap ( Mata 3), S.T.P. Amarante et al .; 3 males , as previous except 23o39'05.3''S , 5o53'51.7''W , 2–5.iv.2001 , Malaise trap (Bosque 6) ( MZUSP , ZMBN ). Diagnostic characters. The species can be separated from the other species in the genus by the following combination of characters: AR 0.45–0.50; virga 8–11 µm long; inferior and superior volsella not well separated; inferior volsella bluntly triangular, without dorsal microtrichia; gonostylus with distinct outer projection and anal point spatulate. Etymology. From Latin triangulus, having three angles, referring to the shape of the gonostylus. FIGURES 14–19. Saetherocladius triangulatus sp. n. , male. 14—tentorium, stipes and cibarial pump; 15—thorax; 16— wing; 17—anal point and tergite IX and dorsal aspect of left gonocoxite and gonostylus; 18—hypopygium with tergite IX and anal point removed, dorsal aspect to the left, ventral aspect to the right; 19—gonostylus with crista dorsalis projecting upwards. Male (n = 4–5, except when otherwise stated). Total length 1.10–1.37, 1.23 mm . Wing length 682–857, 751 µm. Total length / wing length 1.54–1.69, 1.62. Wing length / length of profemur 2.47–2.66, 2.53. Coloration. Thorax and head brown; legs, abdomen and antennae light brown. Head. AR 0.45–0.50, 0.48. Ultimate flagellomere 122–176, 139 µm long. Temporal setae 6–8, 7 including 2–4, 3 inner verticals; 3–4, 3 outer verticals and 0–1, 1 postorbital. Clypeus with 5–6, 6 setae. Tentorium, stipes and cibarial pump as in Figure 14 . Tentorium 77–93, 88 µm long; 12–16, 14 µm wide at sieve pore. Stipes 61–75, 68 µm long; 20–25 (2) µm wide. Palp segment lengths / widths (in µm): 11–16, 14 / 10–12, 11; 23–27, 25 / 11–14, 13; 50–59, 55 / 17–20, 19; 41–54, 47 / 11–14, 12; 81–84 (3) / 9–11 (3). Third palpomere with 3–6, 5 sensilla clavata subapically; longest 8–11, 9 µm long. Thorax ( Fig. 15 ). Antepronotum with 2–4, 3 setae. Dorsocentrals 11–14, 12; acrostichals 9–13, 12 starting close to antepronotum; prealars divided into 3–4, 3 posterior and 2–4, 3 anterior setae; supraalar 1. Scutellum with 4 setae. Wing ( Fig. 16 ). VR 1.37–1.45, 1.40. Costal extension 0–14, 7 µm long. Brachiolum with 1 seta; R with 1– 3, 2 setae; other veins bare. Squama with 0–1, 0 seta. Legs. Spur of fore tibia 31–36, 34 µm long; spurs of mid tibia 12–15, 13 µm and 9–11, 10 µm long; spurs of hind tibia 26–34, 29 µm and 10–14, 12 µm long. Width at apex of fore tibia 20–23, 22 µm; of mid tibia 18– 20, 19 µm; of hind tibia 26–30, 28 µm. Comb with 14–16, 15 setae; longest 23–27, 25 µm long; shortest 13– 16, 15 µm long. Lengths and proportions of legs as in Table 4.

TABLE 4. Lengths (in otherwise stated).	µm) and proportions	of legs of Saetherocladius triangulatus sp. n. , male (n	=	4–5, except when
fe	ti	ta1 ta2 ta3	ta4
p1 263–342, 300	313–400, 348	140–180, 149 72–101, 85 43–68, 52	25–36, 31
p2 270–306, 286	266–310, 282	108–130, 114 43–67 (3) 36–43 (3)	22–27 (3)
p3 292–334, 306	317–367, 332	187–223, 202 85–97, 89 78–94, 85	29–36, 32
continued.
ta5	LR	BV SV BR
p1 22–34, 26	0.39–0.45, 0.43	3.86–4.35; 4.13 4.10–4.72, 4.35 2.0–2.7, 2.3
p2 18–23 (3)	0.40–0.42, 0.41	4.65–4.90 (3) 4.75–5.16, 4.98 2.0–3.1, 2.4
p3 25–34, 29	0.59–0.63, 0.61	3.54–3.65, 3.61 3.07–3.25, 3.15 2.6–3.4, 2.9

Hypopygium ( Figs 17–19 ). Anal point 16–36, 25 µm long; 7–10, 8 µm wide at base; 2–3, 2 µm wide at apex; with 4–6, 5 setae basally. Laterosternite IX with 2–5, 4 setae. Phallapodeme 42–59, 50 µm long; transverse sternapodeme 41–52, 48 µm long. Virga 8–11, 10 µm long. Gonocoxite 91–105, 95 µm long. Gonostylus 50–52, 51 µm long, with large, subrectangular crista dorsalis and distinct outer projection; megaseta 4–8, 6 µm long. HR 1.76–2.00, 1.88. HV 2.19–2.63, 2.43. Female and immatures. Unknown. Distribution and ecology. The species was collected in Malaise traps in Parque Estadual Intervales and in the Reserva Biológica Boracéia, indicating a distribution in the coastal areas of São Paulo State. Both nature reserves have mainly primary Atlantic forest.