Nesterenkonia salmonea sp. nov. and Nesterenkonia sphaerica sp. nov., isolated from the Southern Atlantic Ocean Author Zhang, Gaiyun Author Wang, Lina Author Xie, Fuquan Author Pei, Shengxiang Author Jiang, Li text International Journal of Systematic and Evolutionary Microbiology 2020 2019-11-12 70 2 923 928 journal article 10.1099/ijsem.0.003847 8378fda1-7808-476f-9c03-b551204b03e6 1466-5034 3745040 DESCRIPTION OF NESTERENKONIA SALMONEA SP. NOV. Nesterenkonia salmonea (sal.mo'ne.a. N.L. fem. adj. salmonea dark-salmon-coloured, referring to the colour of the colonies on solid medium). Cells are Gram-stain-positive, aerobic, non-motile and rod-shaped, approximately 0.4–0.5 µm in diameter and 0.7–1.0 µm long. Colonies on MA are approximately 1 mm in diameter, opaque, smooth, circular with regular margins, convex and dark salmon in colour after 6 days at 30 °C. Growth occurs in 0–10% (w/v) NaCl (optimum, 1–4%), at pH 7–12 (pH 9–10) and at 4–40 °C (30–35 °C). Catalase-positive and oxidase-negative. Negative for nitrate reduction, methyl red test, production of H 2 S and melanin, Voges–Proskauer reaction, and hydrolysis of starch, cellulose and casein. Assays using the API ZYM system are positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, α -chymotrypsin and naphthol-AS-BIphosphohydrolase; weakly positive for valine arylamidase, cystine arylamidase and acid phosphatase; negative for N -acetyl- β -glucosaminidase, α -glucosidase, lipase (C14), trypsin, α -galactosidase, β -glucuronidase, β -galactosidase, β -glucosidase, α -mannosidase and β -fucosidase. Analysis using the API 20NE system reveals that cells are positive for hydrolysis of aesculin and gelatin; negative for the remaining 18 substrates. The major menaquinones are MK-7, MK-8 and MK-9. The peptidoglycan type is A4 α , L-Lys-Gly–L-Glu. The polar lipids detected are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid, one unidentified lipid and one unidentified phospholipid. The major fatty acids are anteiso-C 17:0 , anteiso-C 15:0 and anteiso-C 17:1 ; in addition, significant amounts of iso-C 15:0 , iso- C 16:0 and iso-C 17:0 are also present. The genomic DNA G+C content of the type strain is 61.1 mol%. The type strain, GY074 T = (KCTC 39639 T =MCCC 1A11256 T ), was isolated from marine sediment collected from the Southern Atlantic Ocean .