Seven new species of the segmented spider genus Liphistius (Mesothelae, Liphistiidae) in Thailand and Myanmar Author Sivayyapram, Varat https://orcid.org/0000-0002-8466-0270 Center of Excellence in Entomology and Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand Author Kunsete, Chawakorn Center of Excellence in Entomology and Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand Author Xu, Xin https://orcid.org/0000-0001-5632-6622 College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China Author Smith, Deborah R. https://orcid.org/0000-0002-2581-5009 Department of Ecology & Evolutionary Biology, Haworth Hall, 1200 Sunnyside Avenue, University of Kansas, Lawrence, KS 66045, USA Author Traiyasut, Prapun Program in Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani, Thailand Author Deowanish, Sureerat Center of Excellence in Entomology and Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand Author Aung, Mu Mu Forest Research Institute, Forest Department, Ministry of Natural Resources and Environmental Conservation, Yezin, Nay Pyi Taw, Myanmar Author Ono, Hirotsugu https://orcid.org/0000-0002-8568-4549 Department of Zoology, National Museum of Nature and Science, Tsukuba, Ibaraki 305 - 0005, Japan Author Li, Daiqin https://orcid.org/0000-0001-8269-7734 School of Life Sciences, Hubei University, Wuhan, Hubei 430062, China Author Warrit, Natapot https://orcid.org/0000-0002-6338-1782 Center of Excellence in Entomology and Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand & Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan ich108@hotmail.com text ZooKeys 2024 2024-01-16 1189 201 229 http://dx.doi.org/10.3897/zookeys.1189.115850 journal article http://dx.doi.org/10.3897/zookeys.1189.115850 1313-2970-1189-201 7C2F288F95EB410ABF6E0C13990B472F 474DBFBFABDA59DD83E861DE6C4CDD94 Liphistius kaengkhoi Sivayyapram & Warrit sp. nov. Figs 1 , 6 , 7 , 14 Type material. Holotype : Thailand • 1♂; Saraburi, Kaeng Khoi District, Cha Om; alt. 127 m; 14 October 2018; N. Warrit, V. Sivayyapram, C. Kunsete, N. Chatthanabun, P. Traiyasut leg.; ARA-2018-284. Allotype : Thailand • 1♀, same data as for the holotype; ARA-2018-286. Paratypes : Thailand • 2♂ 4♀; same data as for the holotype; ARA-2018-281, ARA-2018-282, ARA-2018-283, ARA-2018-285, ARA-2018-289, ARA-2018-291. Diagnosis. Liphistius kaengkhoi sp. nov. is similar to L. buyphradi sp. nov., L. champakpheaw sp. nov., L. hintung sp. nov., and L. suwat Schwendinger, 1996 in general appearance. The male of L. kaengkhoi sp. nov. can be distinguished from that of L. suwat by the paracymbium not bent outward; and the contrategulum without short blunt cone (Fig. 7 ; Schwendinger 1996 : figs 43, 43A); from those of L. buyphradi sp. nov. and L. champakpheaw sp. nov. by the male palp with swollen paracymbium (flat in L. buyphradi sp. nov., L. champakpheaw sp. nov.; Figs 11 , 13 ); L. kaengkhoi sp. nov. is very similar to L. hintung sp. nov. but can be distinguished by the shorter and finer dentate edge on the proximal margin of the tegulum and shorter paraembolic plate (Figs 7A-D , 9A-D ). The female L. kaengkhoi sp. nov. is difficult to distinguish from those of the L. buyphradi sp. nov., L. champakpheaw sp. nov., L. hintung sp. nov., and L. suwat Schwendinger, 1996. Molecular phylogeny and species delimitation using COI and multi-locus data support monophyly and species status of all new species described here ( Sivayyapram et al. 2023 ). Description. Male (Holotype: ARA-2018-284; Fig. 6A, B ). Coloration (in alcohol) : carapace brown with indistinct black mottling on cephalic region and coxal elevations; abdominal tergites black; membranous part of the opisthosoma cream colored with black mottling on antero-dorsal portion; chelicerae brown, paler on proximal portion; palp and legs brown without distinct annulation. Figure 6. Liphistius kaengkhoi sp. nov. A, B male ARA-2018-284 (holotype) C, D female, ARA-2018-286 (allotype) A, C dorsal view B, D lateral view. Scale bar: 10 mm. Palp (Fig. 7A-D ): tibial apophysis large, carrying one long slender and three tapering megaspines; paracymbium short, almost round, dark patch with spicules isolated by a pale band; cumulus plain, bearing thin black bristles; subtegulum without apophysis; contrategulum without apophysis, distal edge of contrategulum long, slightly invaginate leading to the conical apex; tegulum large, axe-blade shaped, indistinctly separated from the contrategulum by corrugated surface, proximal margin with a short moderate dentate edge, distal margin almost straight, with round apophysis; pigmental bridge between tegulum and contrategulum sigmoid in shape; paraembolic plate projecting to a scale-liked plate, basally wide, leading into short triangular distal margin; embolus proper: sclerotized part with two longitudinal ridges reaching to the truncated apex. Figure 7. Liphistius kaengkhoi sp. nov. male palp and vulva plate A-D ARA-2018-284 (holotype) palp A prolateral view B ventral view C retrolateral view D distal view E, F ARA-2018-286 (allotype) vulva plate E ventral view F dorsal view. Abbreviations: CDO = central dorsal opening; CT = contrategulum; Cu = cumulus; de = distal edge of the contrategulum; Em = embolus; GA = genital atrium; mm = millimeter; PC = paracymbium; PeP = paraembolic plate; PP = poreplate; PS = posterior stalk; RC = receptacular cluster; ST = subtegulum; T = tegulum; TiA = tibial apophysis. Scale bar: 1 mm. Measurements : Total length 11.44; carapace 5.85 long, 5.72 wide; opisthosoma 5.82 long, 5.07 wide; ocular tubercle 0.97 long, 1.17 wide; palpal coxa 2.21 long, 1.30 wide; labium 0.65 long, 1.3 wide; sternum 3.99 long, 1.95 wide (0.91 on ventral surface); palp 12.09 long (3.90 + 2.21 + 3.90 + - + 2.08); leg I 18.72 long (5.72 + 2.73 + 3.77 + 4.42 + 2.08); leg II 19.50 long (5.72 + 2.60 + 3.90 + 4.81 + 2.47); leg III 20.80 long (5.46 + 2.60 + 4.29 + 5.85 + 2.60); leg IV 26.25 long (6.89 + 2.99 + 5.20 + 7.67 + 3.51). Female (Allotype: ARA-2018-286; Fig. 6C, D ). Coloration (in alcohol) : carapace orange with thick black band on the anterior margin and black mottled marking in the posterior portion of the cephalic region and the thoracic region; abdominal tergites cream colored with large mottled black markings; membranous part of the opisthosoma cream with black mottled marking; chelicerae orange; palp and legs: femur to tibia orange, tarsi and metatarsi of legs I-III black, metatarsi of leg IV orange with black annulation on the proximal and distal area. Vulva (Fig. 7E, F ): vulva plate almost round, genital atrium without lateral hair; posterior stalk short, V-shaped; pore plate rectangular, lateral margins thickened and project into lips, anterior margin convex, less thicken, not project into a lip; receptacular clusters racemose, grape-like in shape; central dorsal opening wide. Measurements : Total length 16.2; carapace 6.89 long, 6.50 wide; opisthosoma 9.49 long, 9.67 wide; ocular tubercle 1.04 long, 1.04 wide; palpal coxa 2.60 long, 1.56 wide; labium 0.78 long, 1.95 wide; sternum 3.64 long, 2.21 wide (1.30 on ventral surface); palp 12.87 long (4.68 + 2.47 + 2.86 + - + 2.86); leg I 15.47 long (5.20+ 2.86 + 2.99 + 2.86 + 1.56); leg II 15.99 long (5.20 + 2.73 + 2.99 + 3.25 + 1.82); leg III 16.64 long (5.07 + 2.60 + 3.12 + 3.77 + 2.08); leg IV 23.81 long (7.02 + 3.38 + 4.18 + 6.24 + 2.99). Etymology. The specific epithet kaengkhoi refers to Kaeng Khoi District, the type locality of the new species in Saraburi, Thailand. Distribution. Known only from the type locality. Comment. This new species name was mentioned as Liphistius sp. CK in Sivayyapram et al. (2023) .