Two new freshwater hyphomycetous species of Sporoschisma Berk. & Broome (Chaetosphaeriales) from Tibetan Plateau, China Author Xu, Rong-Ju Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201 (China) and Center of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100 (Thailand) Author Zhou, De-Qun Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201 (China) and Academy of Fanjing Mountain National Park, Tongren University, Tongren 554300 (China) Author Yang, Zhu-Liang Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201 (China) Author Li, Ying Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201 (China) and Center of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100 (Thailand) Author Zhao, Qi Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201 (China) zhaoqi @ mail. kib. ac. cn (corresponding author) zhaoqi@mail.kib.ac.cn text Cryptogamie, Mycologie 2024 2024-02-28 20 3 29 37 https://sciencepress.mnhn.fr/sites/default/files/articles/pdf/mycologie2024v45a3.pdf journal article 291183 10.5252/cryptogamie-mycologie2024v45a3 b5b217f6-fadb-42e4-9717-fd5750b6a238 1776-100X 10835006 Sporoschisma verruculosa R.J.Xu & Q.Zhao , sp. nov. ( Fig. 3 ) TYPE MATERIAL . — China • Tibet , Zayu County , Xiachayu Town ; 28°29’39.2”N , 96°59’35.25”E ; alt. 1537 m ; saprobic on submerged decaying wood in a freshwater stream; 14.VII.2022 ; R . J. Xu ; MD-634 ; holotype : HKAS [ HKAS 129210 ]; ex-type living culture: KUNCC 10461 . ADDITIONAL MATERIAL . — China • Zayu County , Guyu Town ; 28°53’19.64”N , 97°27’49.23”E ; alt. 2805 m ; saprobic on submerged decaying wood in a freshwater stream; 14.VII.2022 ; R . J. Xu ; MD-634-2 ; HKAS [ HKAS 129211 ]; living culture: KUNCC 10462 . ETYMOLOGY . — “verruculosa ” referring the verruculose conidia. INDEX FUNGORUM . — IF900720. FACESOFFUNGI . — FoF 14352. DESCRIPTION Saprobic on submerged decaying wood in a freshwater stream. Sexual morph: Undetermined. Asexual morph: Colonies superficial, effuse, dark brown, with long chains of conidia. Mycelium immersed, composed of pale to dark brown hyphae. Setae 116-204 ×5-9 µm (x̄̄ =161× 6 µm, n= 15), arising from the bulbous base, often with 1-2 at the side of conidiophores, capitate, 2-4-septate, brown, slightly constricted at some septa. Conidiophores 130-320 µm long, stipes 10-18 µm wide below venter and 16-22 µm wide above, 17-33 µm wide at the venter, macronematous, mononematous, erect, straight or slightly flexuous, solitary or 2-3 group, smooth-walled, dark brown to black, cylindrical, a cylindrical stipe and a swollen venter with a long cylindrical neck. Conidiogenous cells monophialidic, integrated, terminal, determinate, dark brown, lageniform, with a tubular collarette and swollen venter, flared margin at free end. Conidia 36-52 × 10-16 µm (x̄̄ =42 ×14 µm, n =25), catenate, emerging in a chain inside the tubular collarette, develop basipetally, guttulate, verruculose, cylindrical, slightly rounded at both ends, with conspicuously darkened septa, hyaline, 0-septate when young, brown to dark brown, 3-septate, when mature, all the cells are the same length. CULTURE CHARACTERISTICS Conidia germinating on PDA within 48 hours and germ tubes produced from both ends. Colonies on PDA reaching 7-10 mm diam at 30 days, with dense, grey, sparse mycelium on surface initially, white grey at the entire; in reverse with a light brown middle and white grey margin. NOTES Morphologically, Sporoschisma verruculosa R.J.Xu & Q.Zhao , sp.nov. shares common characteristics with S. aquaticum in having capitate setae scattered or in groups among conidiophores, cylindrical conidiophores and catenate, cylindrical, brown to dark brown septa, conidia ( Goh et al. 1997 ; Luo et al. 2016 ). However, S. verruculosa R.J.Xu & Q.Zhao , sp. nov. differs from S. aquaticum by having larger sized conidia (36-52 vs 26-32 µm) and verruculose conidia ( Luo et al. 2016 ). Phylogenetically, S. verruculosa R.J.Xu & Q.Zhao , sp. nov. forms a sister lineage with S. chiangraiense with 100% ML/0.99 PP support ( Fig. 1 ). However, S. verruculosa R.J.Xu & Q.Zhao , sp. nov. differs from S. chiangraiense by having 3-septate and verruculose conidia ( Hyde et al. 2019 ).