Siphonophoridae from Brazilian Amazonia Part 1 - The genus Columbianum Verhoeff, 1941 (Diplopoda, Siphonophorida) Author Read, Helen J. Author Enghoff, Henrik text European Journal of Taxonomy 2018 2018-11-20 477 1 23 journal article 22181 10.5852/ejt.2018.477 c447a6b6-36b5-40de-ab76-80a9c7d96d3a 3825218 935D0B09-0508-4771-9575-28C1FE761383 Columbianum adisi sp. nov. urn:lsid:zoobank.org:act: F5FF2BE1-3EDB-4288-BF21-4CB25E8D9EF5 Figs 1 , 6 Diagnosis Small thin species with a clear demarcation between head and rostrum both in lateral and dorsal views. Very rugose with more or less regular and sub-circular tubercles all over the head, metazonites and the channel between the pro- and metazonite, some of these being ‘anchor’-shaped from the side. Limbus with crenulations. Posterior edge to pleurites with saw-shaped teeth, only visible from certain orientations and perhaps due to the tubercles. Accessory claw robust and visible under light microscope. Anterior gonopods with ribbon-like setae on distal segment, posterior gonopods with a simple hyaline tip. Etymology Named in honour of the late Joachim Adis ( 1950–2007 ) who carried out extensive work in Brazilian Amazonia and collected many of the specimens studied here Material studied (11 ♂♂, 18 ♀♀ ) Holotype BRAZIL : ♂, Amazonas Province , Lago Janmauri , 03°20´S , 60°17´W , 14 Sep. 1987 , agua mista, Kempson extractor K10-19, Adis et al . leg. ( INPA ). Paratype BRAZIL : 1 ♀ , same data as for the holotype (INPA). Other specimens (10 ♂♂, 17 ♀♀ ) Locality as above, BE↓50A: 1 Jan. 1987 , 1 Jul. 1987 , 14 Jul. 1987 , 31 Jul. 1987 ; BE↓50B: 15 Jun. 1988 , 1 Jul. 1987 ; BE↓50C: 17 Apr. 1987 , 1 Jul. 1987 , 31 Jul. 1987 ; BE↓50D: 1 Jul. 1987 , 14 Jul. 1987 , 31 Jul. 1987 ; BE↑49: 16 May 1988 ; Kempson extractors (K23-11: 14 Mar. 1988 ; K19-11: 14 Feb. 1988 ; K20- 11: 14 Feb. 1988 ; K29-11: 13 Oct. 1987 ; K20-11: 12 May 1988 ; K26-11: 13 Oct. 1987 ; K11-29: 14 Nov. 1987 (split between INPA, ZMUM, NHMD and NHMUK). Description MEASUREMENTS. Body length ♂: 10–22.5 mm , ♀ : up to 15.5 mm . Body width ♂: 0.59–0.98 mm , ♀ : 0.41– 1.4 mm . Number of podous tergites ♂: 53–78, ♀ : 19–102. Number of apodous tergites: 0–1. COLOUR. An even pale yellow all over, some individuals brownish. Fig. 6. Columbianum adisi sp. nov. A . Head and first few rings of male in dorsal view. B . Dorsal view of midbody ring from male showing detail of tubercles. C . Dorsal view of midbody ring from male showing detail of limbus. D. Midbody ring from male showing hind edge of pleurite from the inside. E . Midbody claw and accessory claw from male in posterior ventral view. F . Male anterior gonopods in situ . G . Male gonopods in lateral view, posterior (left) and anterior (right). H . Lateral view of pleurites from front end of female paratype. Anterior to left. Scale bars = A, D, F = 0.1 mm; B–C, E = 0.01 mm; G–H = 0.2 mm. BODY SHAPE. More or less parallel-sided, perhaps slightly narrower anteriorly. HEAD ( Fig. 6A ). Covered in regular, sub-cylindrical setae, width (between antennal sockets) 0.2– 0.34 mm . With abrupt anterior margin between head and rostrum in dorsal view, head domed in lateral view making clear division between head and rostrum. Length of rostrum from tip to antennal base 0.26–0.5 mm . ANTENNAE. Longer than the body width (1.12:1), appear long in comparison to rostrum, surpassing it by at least one antennal segment and usually substantially more, and over 120% of rostrum length in analysed specimens. Length:width ratio of antennomeres: 0.8, 0.97, 1, 1.03, 0.95, 1.87, 0.5, sensory cones not measured. Sensory pits on antennomeres 5 and 6 clearly present. COLLUM. Anterior margin appearing strongly V-shaped in light microscope but much more gradually curved under SEM, the difference may be due to the relative invisibility of an area without tubercles under light microscope. Tergite 2 length much shorter than collum. MIDBODY SEGMENTS. When plotted this species forms a group with a small body size and a low number of tergites ( Fig. 1 ). Paranota absent. A clear and definite ‘dip’ between pro- and metazonite present. Relative length of pro- and metazonite 0.87–0.92. Surface structure of pro- and metazonites generally very tuberculate. Tubercles appearing more or less regular on the metazonites and in at least one specimen some of the tubercles are like collapsed blisters as seen in C. nahvalr sp. nov. On the anterior margin are tubercles appearing ‘anchor-like’ which may be partly related to the lateral view ( Fig. 6B ). Metazonites with lots of setae. Prozonites with rugose appearance due to cytoscutes but with a smoother region mid-dorsally. ‘Channel’ region between pro- and metazonite with subcircular, clearly pronounced tubercles. Setae short, 0.03–0.07 × body width. Limbus with clear and regular row of semi-circular bumps (under SEM, Fig. 6C ). Pleurites ventrally with notch at least in anterior part of body ( Fig. 6H ); posterior margin saw-like under SEM, only seen from certain angles, maybe due to the tubercles in cross section ( Fig. 6D ). Ozopores present from tergite 5 to the last tergite, on slight protuberance as seen under SEM, this perhaps seems more obvious due to the ‘palisade’ of setae round the rim. LEGS. Length 0.62 ×height. Length:width ratio of podomeres (prefemur to claw): 1.37, 1.5, 0.87, 1.21, 1.21, 3. Tarsus slightly longer than wide. Claw normal. Accessory claw on anterio-ventral side of claw, short and reaching to less than half the length of the claw (22% of claw length on slides) but very robust and visible under light microscope ( Fig. 6E ). Coxal pores present. TELSON. Rounded and of normal shape, length width ratio: 0.33. MALE FIRST PAIRS OF LEGS. First pair thickened, with claw and accessory claw like normal walking legs. ANTERIOR GONOPODS. With five segments (including coxa). Distally simple, the most distal ‘segment’ with flattened ribbon-like setae as seen in SEM and without any apparent groove or other structure medially in ventral side to hold in the posterior gonopods although they are pressed very tightly together. Accessory lobe on ‘segment’ below the knee. Setae abundant on whole structure. POSTERIOR GONOPODS. With an indeterminate number of segments. Distally a simple hyaline tip, with one or more small nicks close to the tip. Remarks Of the three species described here C. adisi sp. nov. and C. nahvalr sp. nov. are the most similar, but C. adisi sp. nov. differs from C. nahvalr sp. nov. by: Table 2. Dates and collections of Columbianum adisi sp. nov. Key: arrow down = caught going down tree trunks; arrow up = caught going up tree trunks; K = collected from Kempson extractors from soil.
Month April 1987 July June May Aug Sept Oct April Mar Feb Jan 1988 Dec Nov May June
Trap type ↓ ↓ K K K K K ↑ ↓
No. specimens 2 16 3 3 2 1 1, 2 3
Condition Inundated Terrestrial Inundated
• smaller body size (overall length, head width and rostrum to antennal length) • thinner body for the number of tergites • more regular tubercles on the pro- and metazonites, also including tubercles that resemble anchors in lateral view. Note however that this could be due to the state of preservation of the specimens looked at or perhaps a function of age/damage during life? • posterior margin to pleurites with very strong saw-like edge (the angle in which this is viewed is critical) • tergites shorter in adisi , and with prozonite shorter relative to metazonite • collum anterior margin less V shaped in adisi In addition, C. adisi sp. nov. lives in a different location – mixed water inundation forest rather than secondary upland forest. Comments on ecology Specimens of C. adisi sp. nov. were only found at Lago Janmauri which is a mixed water site described by Amaral et al . (1997) that is seasonally flooded by river water. They were collected using both Kempson extractors, which sample the soil invertebrates, and arboreal trunk traps that sample the fauna walking up or down trees (for more details of traps see Adis 2002 ). Specimens were caught on trunk traps placed on Hevea spruceana (Spruce ex Benth.) Müll.Arg. or rubber tree ( Euphorbiaceae ), and Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) , with the majority caught on the Hevea . The experiment was designed to follow the movements of various invertebrates as the forest was flooded, see for example Morais et al . (1987) for more details. The distribution of C. adisi sp. nov. in different samples is given in Table 2 . From this it can be seen that the species appears to spend that part of the year when the forest is not inundated in the soil. Once flooding starts it moves onto the trees where it seems to live for the remaining part of the year. The largest sample was in July 1987 when 16 individuals were caught moving down the tree trunks, presumably heading to the ground and the soil at the end of the inundation period. Most of the individuals from the Kempson extractors were very small, suggesting that the eggs are laid in the forest litter or soil and the youngest stages pass their life here. J. Adis (pers. com., 1994) noted that the mixed water forest is regarded as an ecotone between the black and white water inundation forests and that ‘the species should represent in part those occurring in the black water forest at Taruma Mirim’. Columbianum nahvalr sp. nov. was found in the secondary, noninundated forest at Tauma Mirim. Concluding remarks The siphonophorids have a reputation for being difficult to study, and that has been borne out by the current study. While the genus Columbianum seems to be well defined by the shape of the head and rostrum, it has been difficult to find other, reliable characters to distinguish clearly between species, and there is considerable variation within species and even individuals. More information about the postembryonic development might help evaluate the reliability of some of those discussed here.