The ichthyofauna of a poorly known area in the middle-southern Espinhaco mountain range, state of Minas Gerais, Brazil: diagnostics and identification keys Author dos Santos, Sergio Alexandre https://orcid.org/0000-0003-4340-4139 Museu Nacional, Universidade Federal do Rio de Janeiro, Departamento de Vertebrados, Quinta da Boa Vista s / n. CEP 20.940 - 040, Rio de Janeiro, RJ, Brazil sergio.pisces@gmail.com Author de Britto, Marcelo Ribeiro Museu Nacional, Universidade Federal do Rio de Janeiro, Departamento de Vertebrados, Quinta da Boa Vista s / n. CEP 20.940 - 040, Rio de Janeiro, RJ, Brazil text ZooKeys 2021 2021-08-03 1054 25 66 http://dx.doi.org/10.3897/zookeys.1054.67554 journal article http://dx.doi.org/10.3897/zookeys.1054.67554 1313-2970-1054-25 BAEC60898F874A56BAD08E2922E22F60 C0E2746E8DE65F77826FCC7FF2B54A4C Characidium sp. B Fig. 3D Distribution. Upper rio Santo Antonio , rio Doce basin. Diagnosis. Characidium sp. B differs from its congeners from the study area by predorsal length less than 45% of total length; lateral vertical bars absent or without defined shape; dark maculae on caudal fin not forming defined bands. Remarks. In this study, we refer Characidium sp. B such as a second putative new species from the rio Doce basin, due to differences in morphology and color pattern between this species and another from Southeastern Brazil river basins such as C. alipioi , C. grajahuense , C. lagosantense , and C. lauroi .