Two new free-living nematode species of Setosabatieria (Comesomatidea) from the East China Sea and the Chukchi Sea Author Guo, Y. Q. Key Laboratory of Marine fishery resources and Ecological Environment of Fujian Province, Fisheries College, Jimei University, Xiamen, PR China; Author Huang, D. Y. Third Institute of Oceanography, State Oceanic Administration, Xiamen, PR China Author Chen, Y. Z. Key Laboratory of Marine fishery resources and Ecological Environment of Fujian Province, Fisheries College, Jimei University, Xiamen, PR China; Author Wang, J. J. Third Institute of Oceanography, State Oceanic Administration, Xiamen, PR China Author Lin, R. C. Third Institute of Oceanography, State Oceanic Administration, Xiamen, PR China text Journal of Natural History 2015 2015-02-28 49 33 2021 2033 journal article 21130 10.1080/00222933.2015.1006286 7f509167-6b0d-4a3f-a0ba-f9c69808d0fd 1464-5262 3999810 Setosabatieria major sp. nov. ( Figures 3 , 4 and Table 2 ) Type material Five males, one female and one juvenile from station CC4 . Holotype : ♂ 1 on slide number Chukchi20100720 CC42401 . Paratypes : ♂ 2 on slide number Chukchi20100720 CC42401 , ♂ 3 on Chukchi20100720 CC42403 , ♂ 4 on Chukchi20100720 CC42404 , ♂ 5 on Chukchi20100720 CC42405 , ♀ 1 on Chukchi20100720 CC42406 , juvenile on Chukchi20100720 CC42407 . Type locality and habitat Sublittoral zone in the Chukchi Sea (the Arctic Ocean). Latitude: 68.1336°N , longitude: 167.8633°W . Environmental parameters: water depth 52 m , surface water temperature 2.2°C, salinity at 52 m depth 32.13 ‰ . Etymology This species is named for the large body. Figure 3. Setosabatieria major sp. nov. (A) lateral view of male head end; (B) lateral view of female anterior region; (C) lateral view of male tail and copulatory apparatus; (D) spicule and gumbernaculum; (E) lateral view of female tail region. Scale bars: A, D = 20 µm; B, C, E = 50 µm. Figure 4. Setosabatieria major sp. nov. (A) lateral view of male head end, showing cervical setae; (B) lateral view of female head end, showing female amphidial fovea; (C) lateral view of female vulva region, showing vulva and eggs; (D) lateral view of male tail region. Scale bars: A = 25 µm; B = 10 µm; C, D = 50 µm. Table 2. Individual measurements of Setosabatieria major sp. nov. (in µm).

Characters	Holotype	Paratypes
♂1	♂2	♂3	♂4	♂ 5	♀1	Juvenile
Total body length	3130	2720	3075	2930	2700	3145	1730
Head diameter	21	20	22	24	19	23	17
Amphids diameter	12	10	13	14	12	12	8.5
Body width level	22	20	24	26	21	26	9
amphid
Length of cephalic	9	11	11	10	9	11	13
setae (R3)
Nerve ring from the	206	172	199	200	154	188	115
anterior end
Body width level nerve	96	79	76	62	61	75	55
ring
Pharynx length	366	292	327	327	282	332	231
Body width level	117	97	103	93	74	104	70
pharynx end
Maximum body	168	122	136	122	106	176	78
diameter
Spicule length as chord	69	—	69	78	70	—	—
(Spic)
Spicule length as arc	74	—	73	87	73	—	—
Length of gubernaculum	17	—	23	18	23	—	—
apophysis
Anal diameter	82	76	72	68	64	80	46
Tail length	323	290	325	296	241	316	206
Tail length/a.b.d.	4.0	3.8	4.5	4.4	3.7	4.0	4.5
Vulva from anterior	—	—	—	—	—	1390	—
end
Body width level vulva	—	—	—	—	—	164	—
V%	—	—	—	—	—	44	—
a	18.6	22.3	22.7	24.0	25.4	17.9	22.2
b	8.6	9.3	9.4	8.9	9.6	9.5	7.5
c	9.7	9.4	9.5	9.9	11.2	10.0	8.4

Measurements Measurements are given in Table 2 . – 366 W 2806 Holotype ♂ 1: 3130 µm ; a = 18.6, b = 8.6, c = 9.7, Spic = 69 µm 21 117 168 82 – 332 V 2828 Paratype ♀ 1: 3145 µm ; a = 17.9, b = 9.5, c = 8.0, V % = 44.1% 23 104 176 80 Description Body rather large cylindrical, tapering towards both extremities. Cuticle not punctated but with transverse striation most obvious at anterior and posterior extremities. Buccal cavity small cup-shaped, surrounded by six short outer labial, 1.5 – 2 μm long and four cephalic setae, 9 – 13 μm long. Amphidial fovea spiral, with 3.25 – 3.75 turns and occupying 47 – 57% of corresponding body diameter. Four sublateral rows of 10 – 11 cervical setae 8 – 10 μm long. Pharynx gradually swollen posteriorly, not forming a true bulb. Cardia small, muscular, surrounded by intestinal tissue. Ventral gland situated posterior to pharynx and excretory pore about level nerve ring. Nerve ring at 55 – 61% of pharynx length from anterior end. Tail slender, with rather broad, conical anterior portion and rather narrow cylindrical posterior portion. Tail 3.7 – 4.5 times of the anal body diameter with numerous short setae. Tail tip not enlarged, with 15 µm long three terminal setae. Caudal glands not always visible and spinneret well developed. Males. Reproductive system with two opposite outstretched testes. Spicules paired, equal, slight arcuate, 0.9 – 1.3 times as long as cloacal body diameter, with central septum appearing in both proximal and distal portions. Gubernaculum with a pair of short dorsocaudal apophyses. There are 26 – 28 small pre-cloacal papillae supplements, although they are very small and difficult to observe. Females. Similar to male in general characteristics, but tail with fewer short setae and narrow cylindrical posterior portion longer than anterior conical portion. Reproductive system with two outstretched equally developed ovaries. Vulva at 44% of total body length. Differential diagnosis Setosabatieria major sp. nov. is characterized by its rather large body (length 2700 – 3145 µm ; maximum body diameter 106 – 176 µm ), the number (10 – 11) of cervical setae per file, 26 – 28 small papillate precloacal supplements and central septum appearing in both proximal and distal portions of spicules. These characters are obviously different from those of other species in this genus.